Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Identifying Epigenetic Biomarkers for Prostate Cancer

Published: Tuesday, April 01, 2014
Last Updated: Tuesday, April 01, 2014
Bookmark and Share
IDT qPCR assays and probes facilitate the development of predictive epigenetic biomarkers.

In the latest issue of DECODED, Integrated DNA Technologies (IDT) talks about how the prognosis for men with aggressive forms of prostate cancer is improved if detected early.

Dr Antoinette Perry, Senior Research Fellow at the Institute of Molecular Medicine (Dublin, Ireland) leads a team whose research is directed at understanding how changes in DNA methylation of both coding and non-coding RNA genes are involved in driving prostate carcinogenesis.

Previously published work by Dr Perry revealed that SFRP2, a Wnt signalling agonist, is hypermethylated in approximately 65% of prostate tumors.

IDT PrimeTime® qPCR Assays were used to measure expression levels of SFRP2, and subsequent analysis showed an inverse correlation between expression and tumor grade.

As part of a further investigation into the involvement of hypermethylated genes in prostate cancer, prognostic markers are being developed for use in a non-invasive test that will distinguish high-risk from lower-risk tumors. Following cell recovery, IDT primers and ZEN™ Double-Quenched Probes are used for sensitive quantification of prostate cancer-specific DNA methylation.

In addition to the cellular component, the scientists monitor cell-free nucleic acids for DNA methylation and expression of small non-coding RNAs.

The IDT PrimerQuest (idtdna.com/scitools) is being used to design methylation-specific assays (primers and probes that will hybridize specifically to bisulfite modified methylation target regions, but that will not amplify unconverted genomic DNA or unmethylated DNA).


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Dr Patricia Tavormina Named Winner of First IDT ISO 14001 Sustainability Award
Biodiversity research on methane sinks and global warming earns $14,001 in oligo credit.
Thursday, September 10, 2015
Bringing NGS to the Clinic: What Can We Expect?
Dr Elaine Mardis shares her views with IDT on the future of NGS and its role in personalized medicine.
Thursday, February 07, 2013
Understanding and Using Isothermal Gene Assembly for Synthetic Biology
Latest issue of IDT’s Decoded newsletter explains the fundamentals of artificial gene construction.
Tuesday, August 28, 2012
Scientific News
Liquid Biopsies: Miracle Diagnostic or Next New Fad?
Thanks to the development of highly specific gene-amplification and sequencing technologies liquid biopsies access more biomarkers relevant to more cancers than ever before.
Drug - Gene 'One-Two' Punch Against Cancer
Researchers identify gene-drug combinations that, together, target and kill cancer cells while not targeting healthy cells.
Drug Candidates Reduce Abnormal Protein Production
New drug candidates improve cell ability to catch miss-folded proteins that could cause deadly diseases.
Diagnostic Thread - Weaving the Future?
Researchers have created diagnostic threads that could pave the way for next-gen implantable and wearable diagnostics.
Unravelling the Roots of Insect’s Waterproof Coating
Researchers have identified the genes that control cuticular lipid production in Drosophila, by performing an RNAi screen and using Direct Analysis in Real Time and GC-MS.
RNA Suppresses Inflammation
Researchers identify a long noncoding RNA that regulates innate immunity.
Competition to Decipher RNA-Cancer Link
DREAM challenge aims to find the best algorithms for detecting abnormal RNA molecules in cancer cells.
Red Hair Gene Increases Cancer Mutations
Red hair gene variant drives up skin cancer mutations equivalent to that expected from 21 years of sun exposure.
Type 2 Diabetes Genetics Revealed
The largest study of its kind into type 2 diabetes has produced the most detailed picture to date of the genetics underlying the condition.
Blood Cancer Could be Prevented Before it Develops
New research suggests myeloma could be prevented before it develops out of symptomless condition in bone marrow.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!