|MS-Xelerator™: Advanced Algorithms for LC/MS Data Processing Applied to Biomarker Discovery, Differential Analysis and Quantitative Proteomics|
LC-MS based proteomic experiments are used to compare complex biological samples across multiple conditions. Fast, powerful computational tools are needed to explore and detect differences in the areas of Expression Proteomics and Biomarker Discovery. In general, specialized steps are necessary to solve these difficult problems (binning, alignment & normalization, peak picking, relative quantitation, etc.). MS-Xelerator is a collection of software tools dedicated to all of the above tasks.
|Caveolin-1 Expression as a Possible Biomarker in Pancreatic Cancer Diagnosis|
C. Tanase, E. Raducan, L. Albulescu, E. Codorean, M.I. Nicolescu, D.I. Popescu, M.L. Cruceru and A.C. Popa
Caveolin1 (Cav-1) function either as a tumor supressor or as a promoter of metastasis. Overexpresion of cav-1 was correlated with: tumoral grading, proliferration markers (Ki67, p53), serum tumor markers (CEA, CA19.9) and angiogenic markers (VEGF, bFGF).
|A Novel Miniaturized Cell Lysis Device Using Spherically Focused Ultrasound|
Gang Li, Hong Xiao, Min Guo and Jing Cheng
A prototype of miniaturized cell lysis device has been developed using a spherically concave transducer, which is capable of lysing bacteria in absence of added chemical denaturants or enzymes and lysing yeast efficiently without any mechanical or enzymatic pretreatment.
|EuroGentest: Reference Materials for Genetic Testing|
David E Barton, David Gancberg, Philippe Corbisier, Sarah Berwouts, Elisabeth Dequeker and Christine Brady
The lack of reference materials (RMs) for most genetic tests causes difficulties in validating and developing new assays, and results in tests being run without proper quality controls. EuroGentest and the EU-funded network for Medical Genetics, is addressing this issue by defining the present and future needs for RMs, setting priorities for and supporting the development of new RMs and building an enduring network involving all stakeholders in RM development.
|Large-scale Microarray Analysis of Protein and mRNA Level Changes in HL-60 Cells|
Pavel Simara, Irena Koutna, Stanislav Stejskal, Martina Peterkova, Petr Krontorad, Zdenek Rucka
In this study we compare methods for large-scale microarray analysis of protein and RNA level changes in HL-60 cells, responding to differentiation stimuli. Using microarrays we have found, that level of several proteins was either up- or down-regulated after cell differentiation. In some cases there was significant correlation with appropriate genes.
|Preliminary Report: The Geriatric Propamed Study: Prospective pharmacopgenetics in geriatrics|
Dr LS Griffith, Dr L Chialda and Dr A Pahl
In a worldwide first proscpective phamacogenetic study preliminary results show reduction of adverse drug reactions and hospitilisation stays for geriatric patients after pharmacogenetic testing and medication interaction analysis to fit the medicine to the patient.
|Development of a Lab-on-a-Chip for the Characterization of Human Cells |
Richter, L., Stepper, C., Mak, A., Brückl, H. and Ertl, P.
Cell chips are developed to continuously monitor mammalian cell population dynamics in a non-invasive manner. In the presented work we describe the design, fabrication and characterization of a lab-on-a-chip for quantitative cell analysis.
|Label-free Identification of Microorganisms using a Contact-less Dielectric Microsensor|
Ertl, P., Richter, L., Reinthaler, A., Stepper, C., Mak, A., Kast, M., Heer, R. and Brückl, H.
Microfabricated biochips are developed to continuously monitor cell population dynamics in a non-invasive manner. In the presented work we describe the novel combination of contact-less dielectric microsensors and microfluidics to promote biofilm formation for quantitative cell analysis.
|Design, Manufacturing and Test of Disposable Microfluidic System for Blood-Plasma Separation|
M. Kersaudy-Kerhoas, F. Amalou, D. Kavanagh, S. Marson, U. M. Attia, P. Summersgill, T. Ryan and M.P.Y. Desmulliez
Prenatal diagnosis to determine the outcome of pregnancies and detect conditions that may affect future pregnancies has risen as a big issue in the broad public. Analysis of fetal genetic material extracted from maternal blood is a smart alternative to invasive prenatal testing.