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A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope

Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.

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Protein Microarrays for Characterisation of the Bacillus anthracis ‘infectome’
Stephen Kidd, Karen Kempsell, Rebecca Ingram, Pierre Watteau, Daniel Altmann, Michael Elmore, Sue Charlton, Bassam Hallis and Richard Vipond

These studies provide an interesting insight into the transcriptome of virulent B. anthracis in a host infection environment.

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Low cost, low footprint, expandable automated biobanking solutions
James Craven, Chris Morris, Maud Godfrey, Danielle Miller

This case study describes how TTP Labtech’s comPOUND storage modules have been employed by Abcam, a worldwide supplier of antibodies. Here, the turnaround time of sample placement and retrieval is an essential component for high quality service to its customers.

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Kidney Genes Expression in Hypertensive ISIAH Rats
Tatyana Pylnik

The present study was performed to detect the key genes involved in the hypertensive phenotype manifestation in the ISIAH rats with inherited stress-induced arterial hypertension.

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Variation in miRNA expression between TRV-infected tobacco plants is correlated with symptom severity and TRV-16K transcripts abundance
Zhimin Yin, Krystyna Michalak, Miroslawa Chrzanowska

In the present work, expression of certain miRNAs and their targets were investigated in three tobacco plants infected with Tobacco rattle virus (TRV) isolate Deb57 collected from Northern Poland in 2008.

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Application of the SoftFocus® Design Strategy to Epigenetics Targets
Sheppard D, Macritchie J, Allen V & Hughes S

Epigenetics describes the regulation of gene expression that occurs through DNA methylation and post translational modification of histones. The pharmaceutical industry has shown an explosion of interest in this field with new targets emerging.

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Improved Ligation Specificity with Chemically Modified Ligation Components
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.

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Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi

Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.

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Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation
Wu-Bo Li and Michael Goldblatt

Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.

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Showing Results 21 - 30 of 126
Scientific News
Researchers Uncover New Cancer Cell Vulnerability
The research showed that telomerase-expressing cells depend upon a gene named p21 for their survival.
Capturing Cancer: A Powerful New Technique for Early Diagnosis
Researchers describe an innovative technique for early disease detection, which they call immunosignaturing.
No Extra Mutations in Modified Stem Cells, Study Finds
New results ease previous concerns that gene-editing techniques-used to develop therapies for genetic diseases-could add unwanted mutations to stem cells.
Patient-Specific Stem Cells and Personalized Gene Therapy
Patients’ own cells transformed into model for studying disease and developing potential treatment.
Significant Step Towards Blood Test for Alzheimer's
Scientists have identified a set of 10 proteins in the blood which can predict the onset of Alzheimer’s, marking a significant step towards developing a blood test for the disease.
Novel Insights into the Control of Gene Expression
The webinar, “Developing new insights into gene expression using RNA-Seq”, sees Dr Christopher Jones, Research Fellow at the Brighton and Sussex Medical School present novel findings from a recent RNA-Seq project performed by OGT.
Aging Contributes to Rapid Rates of Genomic Change
Signaling challenges for personalized medicine.
CRI Finds Key to Identifying, Enriching Mesenchymal Stem Cells
Biomarker enables researchers to accurately characterize the properties and function of MSCs in the body.
Saturated Fat Intake May Influence Person’s Expression of Genetic Obesity Risk
Limiting saturated fat could help people whose genetic make-up increases their chance of being obese.
Genetic Material Hitchhiking in Our Cells May Shape Physical Traits
Explaining the connection between genotype and phenotype must also consider genetic material that doesn’t come from an organism’s chromosomes at all.
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