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Analytical Pipetting of Serum
John Thomas Bradshaw, PhD, Leah Flumerfelt, Richard H. Curtis, PhD, Rachel Parshley

Many types of chemical and biological analyses are based upon analytical techniques involving accurate delivery of liquid components. Pipetting tools have become commonplace especially in many biological and pharmaceutical laboratories. While these tools are familiar to many, their operational differences when pipetting different types of solutions are often over-looked.

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Droplet Microfluidics for 3D Epithelial Cell Culture
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan

The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.

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Droplet Microfluidics for 3D Epithelial Cell Culture
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan

The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.

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Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Anne F Hammerstein, Diana Caracino, and Paul Wylie

The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.

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Combining low and high volume liquid handling capabilities for ADME screening
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk

In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.

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Does the increase of exosomal microRNAs reflect an activated immune system in melanoma?
Nina Koliha, Florian S. Dreyer , Jochen Dindorf , Andreas Bosio, Andreas S. Baur , and Stefan Wild

Cutaneous malignant melanoma is a form of skin cancer that accounts for 65% of skin cancer-related deaths. The incidence increases continuously, and while early detection leads to nearly 100% survival rates, the mortality raises to greater than 80% for patients with advanced disease.

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A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins
Antti Vasala, BioSilta Oy, Oulu, Finland

The ability to express and extract fully functional proteins in sufficient quantities from bacterial cultures is a prerequisite for many projects in which recombinant proteins are required for structural studies, functional characterization, as assay components or for other applications. However, low yield, poor solubility and lack of functionality are often associated with, what should be, a routine procedure.

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Marking of Weighing Equipment for Hazardous Areas

This poster outlines typical markings for hazardous areas.

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Over-representation of Proteins Identified as Disease Biomarkers and their Relation to Post-Mortem Events
B. Orback, K. Kultima, M. Borén, M. Söderquist and K. Sköld

Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level resulting in loss of sample quality due to post sampling changes. It has recently been reported that the same proteins, regardless of tissue origin or species, are often found expressed differentially in various disease states, bringing into question the significance of these proteins as biomarkers.

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Showing Results 41 - 50 of 157
Scientific News
Study Finds Genetic Clue To Menopause-Like Condition In Young Women
NIH-funded research may also contribute to understanding normal menopause.
How Breast Cancer Cells Break Free To Spread In The Body
Analysis of the downstream signaling pathways of a gene called SNAIL could be used to identify potential targets for scientists who are looking for ways to block or slow metastasis.
Proteins Drive Cancer Cells To Change States
When RNA-binding proteins are turned on, cancer cells get locked in a proliferative state.
Reasons for Malaria’s Drug Resistance
Scientists from Nanyang Technological University (NTU) have discovered exactly how the malaria parasite is developing resistance towards the most important front-line drugs used to treat the disease.
Study Sheds New Light on Relationship Between Personality and Health
Study examine the relationship between certain personality traits and the expression of genes.
Biomarker Discovery Sheds New Light on Heart Attack Risk of Arthritis Drugs
Drug may be given a new lease of life.
Brain Inflammation A Hallmark Of Autism
Johns Hopkins study is largest so far of gene expression in autism brains.
Metal Test Could Help Diagnose Breast Cancer Early
It may be possible to develop a simple blood test that, by detecting changes in the zinc in our bodies, could help to diagnose breast cancer early.
Researchers Conduct Comprehensive Genomic Study of Sub-Saharan Africans
New data resource will enhance disease research and genomic diversity studies.
New Cause of Child Brain Tumour Condition Identified
Doctors and scientists have identified changes in SUFU gene which can cause Gorlin syndrome.
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