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Kidney Genes Expression in Hypertensive ISIAH Rats
Tatyana Pylnik

The present study was performed to detect the key genes involved in the hypertensive phenotype manifestation in the ISIAH rats with inherited stress-induced arterial hypertension.

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Variation in miRNA expression between TRV-infected tobacco plants is correlated with symptom severity and TRV-16K transcripts abundance
Zhimin Yin, Krystyna Michalak, Miroslawa Chrzanowska

In the present work, expression of certain miRNAs and their targets were investigated in three tobacco plants infected with Tobacco rattle virus (TRV) isolate Deb57 collected from Northern Poland in 2008.

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Application of the SoftFocus® Design Strategy to Epigenetics Targets
Sheppard D, Macritchie J, Allen V & Hughes S

Epigenetics describes the regulation of gene expression that occurs through DNA methylation and post translational modification of histones. The pharmaceutical industry has shown an explosion of interest in this field with new targets emerging.

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Improved Ligation Specificity with Chemically Modified Ligation Components
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.

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Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi

Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.

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Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation
Wu-Bo Li and Michael Goldblatt

Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.

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Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs
Pieter Mestdagh, Barbara D’haene, Jan Hellemans and Jo Vandesompele

Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.

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Development of an Automated Platform for HT Cloning and Expression
Stefano Bonacci; Sara Iozzi; Scilla Buccato; Manuele Martinelli; John Telford; Domenico Maione; Roberto Petracca

Biomolecular protocols covering the whole cloning process were implemented in liquid handler robots. When compared to the manual approach, it was found that automation significantly speeds up HT cloning.

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Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces
Abernethy N, Chung E, Fontanelle BT, Gao Y, Jennins D, Koudijs MM, Lim D, Yang L, Ling T, Vukovic P, Wong A, Maeji, NJ

The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.

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Showing Results 51 - 60 of 153
Scientific News
Fast, Low-cost DNA Sequencing Technology One Step Closer To Reality
ASU Regents' Professor Stuart Lindsay led a team of scientists from Arizona State University's Biodesign Institute and IBM's T.J. Watson Research Center in the development of a prototype DNA reader that could make whole genome profiling an everyday practice in medicine.
Developing a Noninvasive Test for Endometriosis
UCSF researchers identify patterns of genetic activity that could help in early detection of disorder.
Breakthrough in Managing Yellow Fever Disease
UC Riverside-led research could lead to antiviral therapeutics and better diagnostics to prevent spread of potentially fatal viral infection.
Almac Announces Launch of CLIA Validated Next Generation Sequencing Assay
P53 considered important biomarker for cancer drug discovery.
Blood Test Could Reduce Antibiotic Use
A new blood biomarker test that indicates whether bacteria is the cause of a patient’s lung infection is now being studied at UPMC Presbyterian.
Light Shed On Genetic Architecture Of Kidney Cancer
Research reveals link between renal cell carcinoma and exposure to aristolochic acid.
Classification of Gene Mutations in Neuroblastoma
Penn Medicine and CHOP experts define riskier mutations in neuroblastoma, setting stage for clinical trial.
Typhoid Gene Unravelled
Natural resistance against typhoid fever is linked to a particular form of the HLA-DRB1 gene.
New Genetic Cause of Epilepsy Identified
San Diego girl improves after discovery of DNA mutation tied to potassium regulation.
First Atlas of Body Clock Gene Expression in Mammals Informs Timing of Drug Delivery
Penn Medicine study has implications for 100 top-selling US drugs, half of which target daily-oscillating genes.
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