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Intact Protein LC-MS: How to Overcome the Challenges?
Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart

Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.

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On-chip quantification of miRNA using digital droplet PCR
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

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High-throughput analysis of protein formulations by DLS
Sophia Kenrick and Daniel Some

High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1

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Digital microphotographic atlas of the adult zebrafish cerebellum
Kristen Lee, Megan Landy, Ashton Cline, Christopher Corbo, and Zoltan Fulop

Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.

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Analytical Pipetting of Serum
John Thomas Bradshaw, PhD, Leah Flumerfelt, Richard H. Curtis, PhD, Rachel Parshley

Many types of chemical and biological analyses are based upon analytical techniques involving accurate delivery of liquid components. Pipetting tools have become commonplace especially in many biological and pharmaceutical laboratories. While these tools are familiar to many, their operational differences when pipetting different types of solutions are often over-looked.

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Droplet Microfluidics for 3D Epithelial Cell Culture
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan

The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.

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Droplet Microfluidics for 3D Epithelial Cell Culture
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan

The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.

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Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Anne F Hammerstein, Diana Caracino, and Paul Wylie

The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.

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Combining low and high volume liquid handling capabilities for ADME screening
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk

In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.

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Showing Results 61 - 70 of 182
Scientific News
After a Sip of Milkshake, Genes and Brain Activity Predict Weight Gain
The new study published in The Journal Neuroscience.
Microchip Captures Clusters of Circulating Tumor Cells
Researchers have developed a microfluidic chip that can capture rare clusters of circulating tumor cells, which could yield important new insights into how cancer spreads.
Study Looks at the Diagnostic Utility of Whole-Genome Sequencing
New research explores the potential of whole genome sequencing to revolutionize the diagnosis of genetic disorders in a clinical setting.
One Molecule at a Time
The ability to study single molecules provides tangible targets for personalised medicine.
Map Diagnostics Developing Mass Spec Test for Down Syndrome
The company hopes that this new test will provide an accessible, affordable and rapid alternative to current screening methods.
Expression of Certain Genes Changes with the Seasons
As the seasons change, so do the expression levels of many human genes, including ones involved in immune function, according to new research.
Drug Perks up Old Muscles and Aging Brains
Whether you’re brainy, brawny or both, you may someday benefit from a drug found to rejuvenate aging brain and muscle tissue.
New Test Could Identify Resistant Tuberculosis Faster
The time needed to genetically sequence the bacteria causing tuberculosis from patient samples has been reduced from weeks to days using a new technique developed by a UCL-led team.
New Insight into Recombination and Sex Chromosomes
Not only does the platypus have some odd physical features, an updated version of its genome has also underscored the unusual genetic characteristics that it harbors.
Gene Variants Show Potential In Predicting Rheumatoid Arthritis Disease Outcomes
Arthritis Research UK-funded scientists at The University of Manchester have identified a new way in which genotyping can be used to predict disease outcomes among sufferers of rheumatoid arthritis.
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