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Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Anne F Hammerstein, Diana Caracino, and Paul Wylie

The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.

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Combining low and high volume liquid handling capabilities for ADME screening
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk

In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.

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Does the increase of exosomal microRNAs reflect an activated immune system in melanoma?
Nina Koliha, Florian S. Dreyer , Jochen Dindorf , Andreas Bosio, Andreas S. Baur , and Stefan Wild

Cutaneous malignant melanoma is a form of skin cancer that accounts for 65% of skin cancer-related deaths. The incidence increases continuously, and while early detection leads to nearly 100% survival rates, the mortality raises to greater than 80% for patients with advanced disease.

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A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins
Antti Vasala, BioSilta Oy, Oulu, Finland

The ability to express and extract fully functional proteins in sufficient quantities from bacterial cultures is a prerequisite for many projects in which recombinant proteins are required for structural studies, functional characterization, as assay components or for other applications. However, low yield, poor solubility and lack of functionality are often associated with, what should be, a routine procedure.

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Marking of Weighing Equipment for Hazardous Areas

This poster outlines typical markings for hazardous areas.

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Over-representation of Proteins Identified as Disease Biomarkers and their Relation to Post-Mortem Events
B. Orback, K. Kultima, M. Borén, M. Söderquist and K. Sköld

Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level resulting in loss of sample quality due to post sampling changes. It has recently been reported that the same proteins, regardless of tissue origin or species, are often found expressed differentially in various disease states, bringing into question the significance of these proteins as biomarkers.

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Cloning And Purification of a Putative Repressor Protein from Arthrobacter nicotinovorans Pao1
Marius Mihasan, Marius Stefan, Hritcu Lucian, Vlad Artenie

The soil bacterium Arthrobacter nicotinovorans carries the pAO1 catabolic megaplasmid which enables it to grow on nicotine (Igloi and Brandsch, 2003).

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Integrating compound storage into automated laboratory workflows
James Craven, Simon Tullett

This poster discusses the benefits of comPOUND for automated sample storage and delivery. TTP Labtech’s pneumatic transport technology, lab2lab, integrates these stores with other instrumentation into managed, fully automated workflows, allowing the scientist to focus on research and data analysis.

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A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope

Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.

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Showing Results 61 - 70 of 175
Scientific News
New Autism-Causing Genetic Variant Identified
Novel approach expected to be useful for other diseases too.
Genetics Help Predict Heart Disease Risk, Statin Benefits
Researchers found that a set of genetic variants could identify people at risk for coronary heart disease and who would benefit most from statin therapy.
Kidney Cancer Detected Early With Urine Test
Washington University School of Medicine researchers have developed a noninvasive method to screen for kidney cancer by measuring the presence of proteins in the urine.
New Gene Influences Apple or Pear Shape, Risk of Future Disease
Duke researchers have discovered that a gene called Plexin D1 controls both where fat is stored and how fat cells are shaped.
Molecule Controls Stem Cell Plasticity By Boosting Gene Expression
Sox9 appears to lead the activation of super-enhancers that boost genes associated with stem cell plasticity.
The First Fine-Scale Genetic Map Of The British Isles
Many people in the UK feel a strong sense of regional identity, and it now appears that there may be a scientific basis to this feeling, according to a landmark new study into the genetic makeup of the British Isles.
Gene Discovery Provides Clues To How TB May Evade The Immune System
The largest genetic study of TB susceptibility to date has led to a potentially important new insight into how the pathogen manages to evade the immune system.
Honey, I Shrunk The Ants: How Environment Controls Size
Ground breaking epigenetics research has implications for everything from cancer to farming.
Blood-Based Genetic Biomarkers Identify Young Boys with Autism
Proof-of-principle method suggests much-earlier diagnoses could be done with clinic test.
Genetically Speaking, Mammals Are More Like Their Fathers
A first of its kind study shows that who we inherit genetic variants from – our mother or father – is crucial for the development of diseases and for research studies aimed at finding causes and potential treatments.
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