|A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins|
Antti Vasala, BioSilta Oy, Oulu, Finland
The ability to express and extract fully functional proteins in sufficient quantities from bacterial cultures is a prerequisite for many projects in which recombinant proteins are required for structural studies, functional characterization, as assay components or for other applications. However, low yield, poor solubility and lack of functionality are often associated with, what should be, a routine procedure.
|Marking of Weighing Equipment for Hazardous Areas|
This poster outlines typical markings for hazardous areas.
|Over-representation of Proteins Identified as Disease Biomarkers and their Relation to Post-Mortem Events|
B. Orback, K. Kultima, M. Borén, M. Söderquist and K. Sköld
Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level resulting in loss of sample quality due to post sampling changes. It has recently been reported that the same proteins, regardless of
tissue origin or species, are often found expressed differentially in various disease states, bringing into question the significance of these proteins as biomarkers.
|Cloning And Purification of a Putative Repressor Protein from Arthrobacter nicotinovorans Pao1|
Marius Mihasan, Marius Stefan, Hritcu Lucian, Vlad Artenie
The soil bacterium Arthrobacter nicotinovorans carries the pAO1 catabolic megaplasmid which enables it to grow on nicotine (Igloi and Brandsch, 2003).
|Integrating compound storage into automated laboratory workflows |
James Craven, Simon Tullett
This poster discusses the benefits of comPOUND for automated sample storage and delivery. TTP Labtech’s pneumatic transport technology, lab2lab, integrates these stores with other instrumentation into managed, fully automated workflows, allowing the scientist to focus on research and data analysis.
|A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor |
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope
Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.
|Protein Microarrays for Characterisation of the Bacillus anthracis ‘infectome’|
Stephen Kidd, Karen Kempsell, Rebecca Ingram, Pierre Watteau, Daniel Altmann, Michael Elmore, Sue Charlton, Bassam Hallis and Richard Vipond
These studies provide an interesting insight into the transcriptome of virulent B. anthracis in a host infection environment.
|Low cost, low footprint, expandable automated biobanking solutions |
James Craven, Chris Morris, Maud Godfrey, Danielle Miller
This case study describes how TTP Labtech’s comPOUND storage modules have been employed by Abcam, a worldwide supplier of antibodies. Here, the turnaround time of sample placement and retrieval is an essential component for high quality service to its customers.
|Kidney Genes Expression in Hypertensive ISIAH Rats|
The present study was performed to detect the key genes involved in the hypertensive phenotype manifestation in the ISIAH rats with inherited stress-induced arterial hypertension.