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Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs
Pieter Mestdagh, Barbara D’haene, Jan Hellemans and Jo Vandesompele

Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.

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Development of an Automated Platform for HT Cloning and Expression
Stefano Bonacci; Sara Iozzi; Scilla Buccato; Manuele Martinelli; John Telford; Domenico Maione; Roberto Petracca

Biomolecular protocols covering the whole cloning process were implemented in liquid handler robots. When compared to the manual approach, it was found that automation significantly speeds up HT cloning.

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Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces
Abernethy N, Chung E, Fontanelle BT, Gao Y, Jennins D, Koudijs MM, Lim D, Yang L, Ling T, Vukovic P, Wong A, Maeji, NJ

The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.

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Gene Expression Profiling: qPCR Toolkit for Quality Control
Švec D., Jacobsson J., Sjöback R., Kubista M.

TATAA Biocenter explain how they developed and optimized high-throughput gene expression qPCR with ValidPrime quality control, compensating for inter-run variations.

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Antigen Determination in Autoimmune Hepatitis Type1
Naveen L Gupta, S Nayak, S Shakeyavar

Objectives of this project were to exploit the database in indian setting to determine nuclear antigens as target for antinuclear antibodies (ANA) in patients of autoimmune hapatitis (AIH) type1.

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Global Gene Expression Changes Induced In Primary Human Hepatocytes By Thiazolidinediones Upon Repeat Dosing of HepatoPac™ Cultures
Michael McVay and Salman R. Khetani

An assessment of global gene expression changes in HepatoPac, a micropatterned co-culture of hepatocytes and stromal cells.

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Is it possible to quantify and rank the quality of several lists of significant genes found with gene expression profiling by different methods?
Joachim R. Grün (1), Andreas Grützkau (1), Biljana Smiljanovic (1), Thomas Häupl (2), Gerd-Rüdiger Burmester (2), Andreas Radbruch (1)

High Performance Chip Data Analysis (HPCDA) improves expression profiling of Affymetrix chips via Bioretis database: you can easily use the default parameters and are sure to get the optimum of true positive results, independant of number of significant genes in your dataset. Gene List Significance Index (GLSI) quantifies quality of gene lists. With GLSI its easily judged which normalization/analyzing method gives better results. HPCDA score ranks by relevance.

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Identification of novel autoantigensin patients with liver autoimmune diseases by Protein MicroArray
C. Zingaretti1, M. Arigò1, A. Cardaci1, A. Sinisi1, L. Muratori3, P. Colombatto4, F. Bonino2, P. Invernizzi5, , A.L. Zignego6 MC. Crosti1, M. Moro1, J. Geginat1, Pagani M.1, R. De Francesco1, S. Abrignani1. & M. Bombaci1

The characterization of autoimmune disease-specific biomarkers are of primary importance for the development of diagnostic tools and the comprehension of pathogenetic mechanisms leading to autoimmunity. To this aim a protein microarray was employed to analyze serum samples from patients with autoimmune hepatitis (e.g. AIH & PBC) and of healthy as controls. A panel of autoantigens able to discriminate among the groups of patients was identified for potential use as biomarkers.

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Intronic polymorphisms in Daucus carota AOX2b generate putative genotype specific miRNA
Hélia G. Cardoso, Maria Doroteia Campos, Birgit Arnholdt-Schmitt

A study in the carrot alternative oxidase gene DcAOX2b from several individual plant genotypes of D. carota cv. Rotin revealed the frequent occurrence of intron length polymorphisms (ILPs). Here we will present an in silico analysis performed in order to identify putative miRNA sites at three different sizes of intron 1. The overall research approach aims to develop functional marker candidates for carrot plant breeding.

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Scientific News
Twins Experiment Reveals Genetic Link with Mosquito Bites
Findings show that attractiveness to insects is based on differences in body odor.
DNA Abnormalities Found in Children with Chronic Kidney Disease
Routine genetic screening of children with CKD could lead to earlier, more precise diagnoses.
Genetic Variance Explains Poor Response to Common Asthma Medications
Study opens door to new therapies for hard-to-treat children.
Gene Variant Linked to Smoking Longer, Getting Lung Cancer Sooner
Smokers with a particular variation in a nicotine receptor gene were more likely to continue smoking for four years.
Systems-Wide Genetic Investigation Of Blood Pressure Regulation
A genetic investigation of individuals in the Framingham Heart Study may prove useful to identify novel targets for the prevention or treatment of high blood pressure.
Genetic Link For Rare Intestinal Cancer
Researchers recommend screening for people with family history.
Breath Test For Detecting Head And Neck Cancer
A portable device can detect the presence of certain types of cancer in people's breath.
Likely Genetic Source Of Muscle Weakness Found
Simple genetic test by TGen reveals likely causes of disease, after other extensive testing failed; one child’s case produces discovery.
Genetic Screening Could Improve Breast Cancer Prevention
A test for a wide range of genetic risk factors could improve doctors’ ability to work out which women are at increased risk of developing breast cancer, a major study of more than 65,000 women has shown.
Brain Tumor Weakness Identified
Discovery could offer a new target for treatment of glioblastoma.
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