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Antibody Array-Based Analysis of Expression Levels in Protein Mixtures Extracted from Formalin-fFxed Paraffin-Embedded (FFPE) Material using ULS Labeling

Protein was extracted from 7-year old FFPE samples of B-cell lymphoma and mamma carcinoma, followed by ULS labeling. Samples were used for single or two color assays using a custom-made antibody array. We observed differential expression levels for several captured analytes. In B-cell lymphoma, expected high levels of IgM were detected, while high levels of CA19-9, a putative marker, were found for the breast cancer tissue.

Date Posted: 12/09/2007

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