Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


5’/3’ RACE Kit, 2nd Generation

Product Description
Robust and reliable amplification of long unknown sequence by RACE.
Obtaining a full-length cDNA is of critical importance for structural and expression studies. The amplification of DNA sequences from a messenger RNA template between a defined internal site and unknown sequences of either the 3' or the 5' end of the mRNA are often referred to as RACE (rapid amplification of cDNA ends), “anchored” PCR, or “one-sided” PCR. Transcriptor Reverse Transcriptase is supplied with the kit to transcribe full-length cDNA up to 14 kb in length and, due to the thermostability of Transcriptor Reverse Transcriptase (up to 65°C), to work with GC-rich templates with high secondary structure. In addition, with Transcriptor Reverse Transcriptase, high sensitivity can be achieved. This results in highly efficient cDNA synthesis and the generation of long RACE products.
Purification of the first-strand cDNA is performed with the High Pure PCR Product Purification Kit. Recombinant Terminal Transferase is used to add a homopolymeric A-tail to the 3' end of the cDNA. The use of poly(A) tail decreases the likelihood of inappropriate trunction by the oligo dT-anchor primer, and due to the weaker A/T binding compared to the G/C binding, longer stretches of A residues are required before the oligo dT-anchor primer will bind to an internal site and truncate the amplification product.
Tailed cDNA is amplified by PCR using a gene-specific primer and the oligo dT-anchor primer. The obtained cDNA is further amplified by a second PCR using a nested specific primer and the PCR anchor primer. As a result, the RACE products can be cloned into an appropriate vector for subsequent characterization studies.
Product 5’/3’ RACE Kit, 2nd Generation
Company Roche - Products Listing
Price Request a quote
More Information View company product page
Catalog Number 03353621001
Quantity 1 kit (10 reactions)
Company Logo

Roche - Products Listing
Roche Diagnostics Corporation Roche Applied Science P.O. Box 50414 9115 Hague Road Indianapolis, IN 46250-0414 US

Tel: +1-800-428-5433
Fax: +1-800-428-2883



Scientific News
Monovar Drills Down Into Cancer Genome
Rice, MD Anderson develop program to ID mutations in single cancer cells.
Autism and Cancer Share a Remarkable Number of Risk Genes
Researchers with the UC Davis Comprehensive Cancer Center, MIND Institute identify more than 40 common genes.
Number Of Known Genetic Risk Factors For Endometrial Cancer Doubled
An international collaboration of researchers has identified five new gene regions that increase a woman’s risk of developing endometrial cancer, one of the most common cancers to affect women, taking the number of known gene regions associated with the disease to nine.
Genetic Risk Factors of Disparate Diseases Share Similar Biological Underpinnings
Penn Institute for Biomedical Informatics and colleagues identify "roadmap" of disease mechanisms to identify candidate drug targets.
Childhood Asthma Research Receives $2M
Research into the impact of a child’s upbringing and social and physical environments on the development of asthma will receive $2 million to tackle the condition that affects as many as one in three Canadians.
Making Precision Medicine a Reality
Researchers are one step closer to understanding the genetic and biological basis of diseases like cancer, diabetes, Alzheimer’s and rheumatoid arthritis – and identifying new drug targets and therapies.
Genetic Markers Influence Addiction
Differences in vulnerability to cocaine addiction and relapse linked to both inherited traits and epigenetics, U-M researchers find.
Potential “Good Fat” Biomarker
New method to measure the activity of energy consuming brown fat cells could ease the testing weight loss drugs.
New Insights into Gene Regulation
Researchers have solved the three-dimensional structure of a gene repression complex that is known to play a role in cancer.
Controlling RNA in Living Cells
Modular, programmable proteins can be used to track or manipulate gene expression.

Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!