Gene-specific probes and primer for PGK1 (phosphoglycerate kinase 1) for quantification of gene expression levels using dual-color real-time PCR.
Each Universal ProbeLibrary Reference Gene Assay provides a specifically designed 12-mer UPL reference gene probe and the corresponding reference gene-specific primer pair. Labeled with LightCycler® Yellow 555 at the 5' end and a dark quencher dye near the 3' end, the UPL reference gene probes can be detected using real-time PCR instruments with excitation filters of 470 nm to 530 nm and emission filters of 550 nm to 610 nm. In multiplex assays, a standard FAM or SYBR Green I filter is used as the first channel to detect FAM-labeled UPL probes for the gene of interest, and a longer-wavelength emission filter (usually 560 nm or 568 nm) is used as the second channel to detect UPL reference gene probes.
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