Corporate Banner
Satellite Banner
Technology Networks Header
Wednesday, September 17, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Challenges of Analyzing Single Cell Gene Expression Data
  Videos

Return

Challenges of Analyzing Single Cell Gene Expression Data
Fluidigm Corporation

Kenneth Livak, Senior Scientific Fellow, Fluidigm

Abstract
Single cell researchers are currently using the Fluidigm BioMark System to measure gene expression levels for 10s to 100s of genes in 100s to 1000s of samples.  Data from single eukaryotic cells show cell-to-cell variation in mRNA amounts that ranges from 10- to 1000-fold depending on the gene and type of cell.  These results fit the stochastic model that eukaryotic transcripts are produced in short but intense bursts interspersed with intervals of inactivity.  This noise inherent in single cell gene expression challenges conventional methods for obtaining and analyzing qPCR data.  Factors such as replicates, limit of detection, normalization, data display, and univariate versus multivariate analysis need to be re-evaluated.  Another important consideration is that data needs to be collected from a statistically significant number of single cells and for at least tens to hundreds of genes.  By acknowledging and addressing the intrinsic noise, single-cell gene expression profiling can be used to gain biological insights that are just not possible when measuring average expression levels from hundreds or thousands of cells.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Genetic Testing for Prostate Cancer
Genetic testing can identify men at six-fold increased risk of prostate cancer.
Zebrafish Genes Linked To Human Respiratory Diseases
Genes have been discovered which may be synonymous with the genes for developing hair-like structures in the human airway.
Ancient Vertebrate Uses Familiar Tools
Sea lamprey studies show remarkably conserved gene expression patterns in jawless versus jawed vertebrates.
Study Aims to Develop Breath Test for COPD
A new clinical iniative, lead by NYU Langone Medical Center, aims to determine a breath test’s effectiveness to identify volatile organic compounds (VOCs) in human breath that are biomarkers of chronic obstructive pulmonary disease (COPD).
Clinical Utility of Myriad myPath(TM) Melanoma Test Demonstrated
Test reduced indeterminate cases by 76 percent and changed treatment in 35 percent of cases.
In Directing Stem Cells, Context Matters
The surface cells are grown on has a profound effect on differentiation.
New Gene Mutations for Wilms Tumor
UT Southwestern researchers identified the mutated genes by sequencing the DNA of 44 tumors.
‘Switching off’ Autoimmune Diseases
Scientists have made an important breakthrough in the fight against debilitating autoimmune diseases by revealing how to stop cells attacking healthy body tissue.
Repressing the Repressors May Drive Tissue-Specific Cancers
Stowers scientists establish Drosophila and mammalian models to study mutations found in pediatric brain tumors.
Scientists Looking Across Human, Fly and Worm Genomes Find Shared Biology
Studies reveal powerful commonalities in biological activity and regulation among species.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv