Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>Videos>This Video
  Videos

Return

Challenges of Analyzing Single Cell Gene Expression Data
Fluidigm Corporation

Kenneth Livak, Senior Scientific Fellow, Fluidigm

Abstract
Single cell researchers are currently using the Fluidigm BioMark System to measure gene expression levels for 10s to 100s of genes in 100s to 1000s of samples.  Data from single eukaryotic cells show cell-to-cell variation in mRNA amounts that ranges from 10- to 1000-fold depending on the gene and type of cell.  These results fit the stochastic model that eukaryotic transcripts are produced in short but intense bursts interspersed with intervals of inactivity.  This noise inherent in single cell gene expression challenges conventional methods for obtaining and analyzing qPCR data.  Factors such as replicates, limit of detection, normalization, data display, and univariate versus multivariate analysis need to be re-evaluated.  Another important consideration is that data needs to be collected from a statistically significant number of single cells and for at least tens to hundreds of genes.  By acknowledging and addressing the intrinsic noise, single-cell gene expression profiling can be used to gain biological insights that are just not possible when measuring average expression levels from hundreds or thousands of cells.

Request more information
Company product page

Scientific News
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Smart Material Hunts Cancers
Team has created smart material that locates and images cancer or tumour sites in tissue.
Peer Reviewed Study Demonstrates Mass Spec Technique
The peer reviewed study demonstrates MS workflow, TMTCalibrator workflow, which dramatically enhances detection of key early stage Alzheimer’s biomarkers.
Stem Cells Growing 3D Lung-in-a-Dish
Researchers have created 3D lung-like tissue from lung-derived stem cells. The tissue can be used to study lung diseases.
The Genetics of Blood Pressure
Researchers have identifed areas of the genome associated with blood-pressure including 17 previously unknown loci.
Mystery of Early Embryonic Development Solved
Breakthrough genomics technique can be used to map epigenetic marks across the genome using fewer cells and will benefit cancer research
Mouse Genes Guiding Precision Medicine
Research of the mouse genome has identified hundreds of essential-to-life genes from the 1751 genes studied.
Eisai Establishes AiM Institute
The Andover innovative Medicines (AiM) Institute will develop innovative precision medicines for hard-to-treat conditions.
Schizophrenia Drug Response Dictated by Genes
Study suggests schizophrenia drug may decrease negative symptoms for people with certain gene variant.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!