Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Join | Sign in
Home>Videos>This Video
  Videos

Return

BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Tumor Extracellular Vesicles
Massachusetts General Hospital

Development of biofluid-based molecular diagnostic tests for cancer is an important step towards tumor characterization and real-time monitoring in a minimally invasive fashion. Extracellular vesicles (EVs) are released from tumor cells into body fluids and can provide a powerful platform for tumor biomarkers since they carry tumor proteins and nucleic acids. BEAMing (beads, emulsion, amplification, magnetics) PCR is substantially more sensitive than many other assays for detecting single nucleotide mutations when there is a significant background of wild-type sequences. Picodroplet digital PCR developed by RainDance Technologies is another highly sensitive technology that can be used to quantify rare mutations in a high wild type background. Here, we describe a novel approach that uses BEAMing droplet RT-PCR, as well picodroplet digital RT-PCR, to interrogate mRNA sequences contained within EVs from serum and cerebrospinal fluid (EV-BEAMing). Using both assays, we were able to reliably detect and quantify mutant and wild-type IDH1 transcripts in cerebrospinal fluid of patients with gliomas. EV-BEAMing and picodroplet dPCR are valuable new tools for cancer diagnostics which can be applied to a variety of biofluids and neoplasms.

Request more information
Company product page


Access to this article and other content is for registered users.

Join the Technology Networks Community

  • Access to the latest scientific news, products and research through Technology Networks
  • Upload and share your posters on ePosters
  • View a library of 1,800+ scientific and medical posters
  • A library of 3,000+ scientific videos on LabTube


Sign In



Forgotten your details? Click Here
If you already have an account with Technology Networks, please use your existing login details. If you do not yet have an account please join here.

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
New Genetic Form of Obesity and Diabetes Discovered
Scientists have discovered a new inherited form of obesity and type 2 diabetes in humans.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
New Genetic Form of Obesity and Diabetes Discovered
Scientists have discovered a new inherited form of obesity and type 2 diabetes in humans.
Epigenetic Switches that turn Stem Cells into Blood Vessel Cells Uncovered
Researchers at the University of Illinois have identified a molecular mechanism that directs embryonic stem cells to mature into endothelial cells.
Study Finds Addition of Epigenetic Data Improves Predictions of Complex Traits
Researchers reported that combining genetic and epigenetic associations can bolster phenotypic predictions.
Reducing Uncertainties in Genetic Tests
Breast cancer-associated gene study shows potential to sort out harmful and harmless variants.
Is the Amount of Sleep Each of Us Needs Genetic?
Scientists have recently reported on the discovery of two genes, originally known for their roles in cell division, that are required for normal sleep patterns in Drosophila melanogaster.
New Immunoregulation and Biomarker
Clinicians at LMU have elucidated a mechanism involved in determining the lifespan of antibody-producing cells, and identified a promising new biomarker for monitoring autoimmune diseases.
Vulnerabilities in Genome's 'Dimmer Switches' Should Shed Light on Hundreds of Complex Diseases
Up to one-fifth of human DNA act as dimmer switches for nearby genes, but scientists have long been unable to identify precisely which mutations in these genetic control regions really matter in causing common diseases.
Novel Epigenetic Technique Opens Door to Combatting Virulent Strains of Bacteria
Researchers from the Icahn School of Medicine at Mount Sinai say they have developed a novel method to more precisely analyze bacterial populations and reveal epigenetic mechanisms that can drive virulence.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters