Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>Videos>This Video
  Videos

Return

Extreme PCR: Robust, Efficient Amplification in < 1 Min
SELECTBIO

Rapid-cycle PCR was introduced in 1990, reducing 2-4 hours of amplification to 10-15 min. Since that time, incremental progress has been made in reducing amplification times below 10 minutes, although yield and PCR efficiency often suffer at higher speeds. We now introduce, “Extreme PCR”, a method that combines <2 s thermal cycles with chemistry modifications to enhance the completion of annealing and extension segments. Specifically, primer and polymersase concentrations are increased so that primer annealing and complete extension near 100% efficiency each cycle. Cycle times of less than 1 s were obtained for PCR products less than 100 bp, resulting in specific, high-yield amplification as demonstrated by gels and melting curves. In addition, we introduce a new method for measuring polymerase activity under PCR conditions. Performed on real-time instruments, the effect of various components on extension rates reveal that monovalent cations, most Tm depressants, secondary structure, probes and dyes inhibit extension, while Mg++, temperature, GC content, and DMSO have local maxima. By studying the effect of different PCR components on extension rate, amplification speed can be optimized.

Request more information
Company product page

Scientific News
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
NCI Collaborates with Multiple Myeloma Research Foundation
NCI collaborates with MMRF to incorporate genomic and clinical data into NCI Genomic Data Commons database.
Probe Identifies Schizophrenia Genes That Stunt Brain Development
Scientists have isolated schizophrenia-related gene variants that change gene expression in the brain.
CES Score May Predict Response to Cancer Treatment
Researchers identify new type of biomarker that helps predict prognosis and response to several types of cancer treatment.
Heart Arrhythmia Caused by Mosaic of Mutant Cells
Researchers have solved the genetic mystery of an infant suffering from heart arrhythmia.
New Therapeutic Target for Crohn’s Disease
A promising new target for drugs that treat IBD has been identified along with a possible biomarker for IBD severity.
Uncovering Water Bear Resilience
A protein identified in water bears can protect DNA of human cells from lethal doses of radiation damage.
Peer Reviewed Study Demonstrates Mass Spec Technique
The peer reviewed study demonstrates MS workflow, TMTCalibrator workflow, which dramatically enhances detection of key early stage Alzheimer’s biomarkers.
Smart Material Hunts Cancers
Team has created smart material that locates and images cancer or tumour sites in tissue.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!