Corporate Banner
Satellite Banner
Technology Networks Header
Friday, July 25, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Extreme PCR: Robust, Efficient Amplification in < 1 Min
  Videos

Return

Extreme PCR: Robust, Efficient Amplification in < 1 Min
SELECTBIO

Rapid-cycle PCR was introduced in 1990, reducing 2-4 hours of amplification to 10-15 min. Since that time, incremental progress has been made in reducing amplification times below 10 minutes, although yield and PCR efficiency often suffer at higher speeds. We now introduce, “Extreme PCR”, a method that combines <2 s thermal cycles with chemistry modifications to enhance the completion of annealing and extension segments. Specifically, primer and polymersase concentrations are increased so that primer annealing and complete extension near 100% efficiency each cycle. Cycle times of less than 1 s were obtained for PCR products less than 100 bp, resulting in specific, high-yield amplification as demonstrated by gels and melting curves. In addition, we introduce a new method for measuring polymerase activity under PCR conditions. Performed on real-time instruments, the effect of various components on extension rates reveal that monovalent cations, most Tm depressants, secondary structure, probes and dyes inhibit extension, while Mg++, temperature, GC content, and DMSO have local maxima. By studying the effect of different PCR components on extension rate, amplification speed can be optimized.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Researchers Uncover New Cancer Cell Vulnerability
The research showed that telomerase-expressing cells depend upon a gene named p21 for their survival.
Capturing Cancer: A Powerful New Technique for Early Diagnosis
Researchers describe an innovative technique for early disease detection, which they call immunosignaturing.
No Extra Mutations in Modified Stem Cells, Study Finds
New results ease previous concerns that gene-editing techniques-used to develop therapies for genetic diseases-could add unwanted mutations to stem cells.
Patient-Specific Stem Cells and Personalized Gene Therapy
Patients’ own cells transformed into model for studying disease and developing potential treatment.
Significant Step Towards Blood Test for Alzheimer's
Scientists have identified a set of 10 proteins in the blood which can predict the onset of Alzheimer’s, marking a significant step towards developing a blood test for the disease.
Novel Insights into the Control of Gene Expression
The webinar, “Developing new insights into gene expression using RNA-Seq”, sees Dr Christopher Jones, Research Fellow at the Brighton and Sussex Medical School present novel findings from a recent RNA-Seq project performed by OGT.
Aging Contributes to Rapid Rates of Genomic Change
Signaling challenges for personalized medicine.
CRI Finds Key to Identifying, Enriching Mesenchymal Stem Cells
Biomarker enables researchers to accurately characterize the properties and function of MSCs in the body.
Saturated Fat Intake May Influence Person’s Expression of Genetic Obesity Risk
Limiting saturated fat could help people whose genetic make-up increases their chance of being obese.
Genetic Material Hitchhiking in Our Cells May Shape Physical Traits
Explaining the connection between genotype and phenotype must also consider genetic material that doesn’t come from an organism’s chromosomes at all.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv