Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Illumina Introduces TruSeq® Stranded mRNA and Total RNA Sample Preparation Kits

Published: Wednesday, September 19, 2012
Last Updated: Wednesday, September 19, 2012
Bookmark and Share
Delivers superior data quality for challenging samples, unprecedented view of the transcriptome.

Illumina has introduced TruSeq Stranded mRNA and Total RNA Sample Preparation Kits for RNA sequencing.

The new reagent kits enable researchers to quickly and easily conduct gene expression studies that provide a complete view of the transcriptome, even from low-quality RNA samples, such as formalin-fixed, paraffin-embedded (FFPE) samples.

The Total RNA Sample Preparation Kit efficiently removes ribosomal RNA and other high abundance transcripts using Epicentre's proven Ribo-Zero™ ribosomal RNA reduction chemistry, with an improved workflow optimized for high-throughput studies.

The resulting combination of high-quality ribosomal removal and sample preparation chemistries into a single, streamlined solution provides an exciting new opportunity for researchers to conduct highly-accurate gene expression studies.

"TruSeq Stranded mRNA and Total RNA Sample Preparation Kits offer powerful, high-throughput, and easy-to-use reagent solutions suited to a broad range of study designs, including those that use challenging but important FFPE samples," said Christian Henry, Senior Vice President and General Manager of Illumina's Genomic Solutions Business.

Henry continued, "These types of samples are a rich source of information on changes in gene expression and regulation associated with cancer and a wide range of other diseases."

The TruSeq Stranded RNA Sample Preparation Kits are available in multiple configurations to suit a wide range of study design needs, with offerings for both mRNA-focused and whole-transcriptome analysis, as well as for both small and large sample sizes.

Configurations compatible with human, mouse and rat samples are currently available, with additional offerings planned later this year for plant samples and for the effective removal of globin transcripts from blood samples.

"Illumina's new RNA sequencing kits allow researchers to quickly and robustly analyze nearly every aspect of the transcriptome and will certainly be a powerful new tool in unraveling the complexities of gene regulation," said Brenton Graveley, professor at University of Connecticut Health Centre and researcher in the modENCODE Project.

Graveley continued, "We were able to reliably detect expression changes in coding as well as non-coding RNA species, for which important regulatory roles impacting a rapidly broadening array of phenotypes continue to be discovered."

The TruSeq Stranded mRNA and Total RNA Sample Preparation Kits are the newest additions to the TruSeq line of sample prep solutions, which are designed to further advance data quality, ease-of-use, and the cost effectiveness of sequencing. Additional benefits of TruSeq Stranded mRNA and Total RNA Sample Preparation Kits include:

•An improved workflow, requiring less hands-on time while delivering the same high standard of performance and affordability as Illumina's current TruSeq products.
•High strand specificity and uniform coverage in a comprehensive end-to-end solution.
•Availability in multiple configurations, including 48-sample low-throughput kits containing 12 of 24 total available indexes, and 96-sample high-throughput kits. Additionally, the company recently introduced TruSeq DNA HT Sample Preparation Kits, which contain 96 unique index pairs pre-loaded into 96-well plates.

TruSeq Stranded mRNA and Total RNA Sample Preparation Kits are now shipping.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,400+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Potential “Good Fat” Biomarker
New method to measure the activity of energy consuming brown fat cells could ease the testing weight loss drugs.
MicroRNA Pathway Could Lead to New Avenues for Leukemia Treatment
Cancer researchers at the University of Cincinnati have found a particular signaling route in microRNA (miR-22) that could lead to targets for acute myeloid leukemia, the most common type of fast-growing cancer of the blood and bone marrow.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Doubling Down on Dengue
HMS researchers have discovered two ways a compound blocks dengue virus.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
AstraZeneca to Sequence 2 Million Genomes in Search for New Drugs
Company launches integrated genomics approach which aims to transform drug discovery and development.
Unique Model for Studying ALS
Unique mouse model will allow researchers to better study the genetic origins and potential treatments of ALS.
Targeting an ‘Undruggable’ Cancer Gene
RAS genes are mutated in more than 30 percent of human cancers and represent one of the most sought-after cancer targets for drug developers.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.
Improving Engineered T-Cell Cancer Treatment
Purdue University researchers may have figured out a way to call off a cancer cell assassin that sometimes goes rogue and assign it a larger tumor-specific "hit list."
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!