Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Identification of Plasma Lipid Biomarkers for Prostate Cancer by Lipidomics and Bioinformatics

Published: Monday, April 08, 2013
Last Updated: Monday, April 08, 2013
Bookmark and Share
This study explores a new approach to seeking biomarkers for prostate cancer and other diseases.

Background:
Lipids have critical functions in cellular energy storage, structure and signaling. Many individual lipid molecules have been associated with the evolution of prostate cancer; however, none of them has been approved to be used as a biomarker. The aim of this study is to identify lipid molecules from hundreds plasma apparent lipid species as biomarkers for diagnosis of prostate cancer.

Methodology/Principal Findings:
Using lipidomics, lipid profiling of 390 individual apparent lipid species was performed on 141 plasma samples from 105 patients with prostate cancer and 36 male controls. High throughput data generated from lipidomics were analyzed using bioinformatic and statistical methods. From 390 apparent lipid species, 35 species were demonstrated to have potential in differentiation of prostate cancer. Within the 35 species, 12 were identified as individual plasma lipid biomarkers for diagnosis of prostate cancer with a sensitivity above 80%, specificity above 50% and accuracy above 80%. Using top 15 of 35 potential biomarkers together increased predictive power dramatically in diagnosis of prostate cancer with a sensitivity of 93.6%, specificity of 90.1% and accuracy of 97.3%. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) demonstrated that patient and control populations were visually separated by identified lipid biomarkers. RandomForest and 10-fold cross validation analyses demonstrated that the identified lipid biomarkers were able to predict unknown populations accurately, and this was not influenced by patient's age and race. Three out of 13 lipid classes, phosphatidylethanolamine (PE), ether-linked phosphatidylethanolamine (ePE) and ether-linked phosphatidylcholine (ePC) could be considered as biomarkers in diagnosis of prostate cancer.

Conclusions/significance:
Using lipidomics and bioinformatic and statistical methods, we have identified a few out of hundreds plasma apparent lipid molecular species as biomarkers for diagnosis of prostate cancer with a high sensitivity, specificity and accuracy.

The article is published online in the journal PLoS ONE and is free to access.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Advanced Cell Culture Techniques for Cancer Drug Discovery
This review focuses on the culturing of cell lines representative of solid cancers in advanced cell culture conditions.
Thursday, June 12, 2014
Application of MALDI-TOF Mass Spectrometry in Clinical Virology
MS methods can be used currently for viral diagnosis but refinement of technology will be an important approach for the introduction of MALDI-TOF in routine viral diagnosis.
Monday, December 16, 2013
PCR-based Pool Screening Method for High Throughput Wuchereria Bancrofti Detection
The purpose of this study was to improve the DNA extraction protocol and qPCR assay to achieve the detection of a single mf in a pool of at least twelve 60-µl blood samples.
Wednesday, April 24, 2013
A Hybrid Structural Approach to Analyze Ligand Binding by the 5-HT4 Receptor
This study reveals the potential of hybrid structural methods, including mass spectrometry to probe physiological and functional GPCR-ligand interactions with purified native protein.
Friday, February 15, 2013
Simultaneous Saccharification and Co-Fermentation for Bioethanol Production at Lab, PDU and Demo Scales
In this study, researchers designed a SSCF process and scaled up from lab to demo scale reaching 4% (w/v) ethanol using xylose rich corncobs.
Monday, February 04, 2013
Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection
Exploiting Red-mediated recombineering to perform scanning mutagenesis using synthetic oligonucleotide libraries.
Wednesday, June 06, 2012
Binding-site Assessment by Virtual Fragment Screening
This article reports the results of virtually screening ~11,000 diverse fragment-like compounds against a total of 152 protein binding sites.
Wednesday, June 06, 2012
FTMAP: Extended Protein Mapping with User-selected Probe Molecules
A direct computational analogue that can be used to sample the surface of a target protein using small organic molecules as probes.
Wednesday, May 23, 2012
A Comparative Study of Fragment Screening Methods
New methods and insights into fragment screening on the p38a kinase.
Tuesday, May 22, 2012
Specificity Quantification of Biomolecular Recognition and its Implication for Drug Discovery
Highly efficient and specific biomolecular recognition requires both affinity and specificity. Here scientists developed a novel method SPA (SPecificity and Affinity) based on funneled energy landscape theory, with increased specificity on previous models.
Monday, March 26, 2012
Structural Studies of Specific Intermolecular Interactions and Self-Aggregation of Biomolecules and Their Application to Drug Design
The group of scientists performed structural analyses on biologically important molecules and their complexes with partners, hoping to contribute to molecular design for controlling biologically abnormal functions.
Monday, March 26, 2012
Core Site-Moiety Maps Reveal Inhibitors and Binding Mechanisms of Orthologous Proteins by Screening Compound Libraries
Study reveals the CoreSiMMap is useful to identify adaptive SK inhibitors and provide insight into the binding mechanisms of compounds.
Friday, March 09, 2012
A High-Throughput Screen Identifies a New Natural Product with Broad-Spectrum Antibacterial Activity
Researchers from Children's Hospital, Boston report the development of a sensitive and robust but low-tech and inexpensive high-throughput metabolic screen for novel antibiotics.
Wednesday, March 07, 2012
Esophageal Cancer Metabolite Biomarkers Detected by LC-MS and NMR Methods
Metabolic profiles derived from the combination of LC-MS and NMR methods were shown to readily distinguish EAC patients and potentially promise important routes to understanding the carcinogenesis and detecting the cancer.
Tuesday, March 06, 2012
The Benefits of Constructing Leads from Fragment Hits
Since it is now clear that fragments can be developed into clinical candidates, the discussion is shifting to the design of good-quality lead compounds from fragment hits.
Monday, February 27, 2012
Scientific News
Promising Class of New Cancer Drugs Cause Memory Loss in Mice
New findings from The Rockefeller University suggest that the original version of BET inhibitors causes molecular changes in mouse neurons, and can lead to memory loss in mice that receive it.
Electrical Control of Cancer Cells
Research led by scientists at The University of Texas Health Science Center at Houston (UTHealth) has revealed a new electrical mechanism that can control these switches.
Signature of Microbiomes Linked to Schizophrenia
Studying microbiomes in throat may help identify causes and treatments of brain disorder.
Inflammation Linked to Colon Cancer Metastasis
A new Arizona State University research study led by Biodesign Institute executive director Raymond DuBois has identified for the first time the details of how inflammation triggers colon cancer cells to spread to other organs, or metastasize.
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
Determining the Age of Fingerprints
Watch the imprint of a tire track in soft mud, and it will slowly blur, the ridges of the pattern gradually flowing into the valleys. Researchers have tested the theory that a similar effect could be used to give forensic scientists a way to date fingerprints.
Genetic Overlapping in Multiple Autoimmune Diseases May Suggest Common Therapies
CHOP genomics expert leads analysis of genetic architecture, with eye on repurposing existing drugs.
Surprising Mechanism Behind Antibiotic-Resistant Bacteria Uncovered
Now, scientists at TSRI have discovered that the important human pathogen Staphylococcus aureus, develops resistance to this drug by “switching on” a previously uncharacterized set of genes.
Tissue Bank Pays Dividends for Brain Cancer Research
Checking what’s in the bank – the Brisbane Breast Bank, that is – has paid dividends for UQ cancer researchers.
Researchers Publish Landmark “Basket Study”
Researchers from Memorial Sloan Kettering Cancer Center (MSK) have announced results from the first published basket study, a new form of clinical trial design that explores responses to drugs based on the specific mutations in patients’ tumors rather than where their cancer originated.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!