Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Leica Microsystems Releases Application Suite Advanced Fluorescence 4.0

Published: Friday, June 13, 2014
Last Updated: Thursday, June 12, 2014
Bookmark and Share
Image analysis, environmental control and experiment handling via mobile devices for life science research.

Leica Microsystems has launched the Leica Application Suite Advanced Fluorescence (LAS AF), the software platform for advanced life science research in widefield and confocal microscopy, in its 4.0 version.

With LAS AF researchers can now perform 2D image analysis on multi-channel experiments and fully control the environmental conditions of an experiment. In addition, users of widefield systems can now connect to the acquisition station remotely anywhere at any time.

Wizard for 2D Image Analysis
A workflow guides users step by step through the 2D image analysis from applying filters, thresholding, and binary image processing, to measurements and classification. Results are obtained easily, quickly, and reproducibly even with multi-dimensional data sets. Each analysis step offers a set of tools that researchers can choose according to the analysis requirements. The image viewer provides immediate feedback on the applied settings. Analysis workflows can be saved as user-defined protocols and applied to further data sets at a later time. The analysis results can be saved with the experiment or exported as Excel reports together with histograms and images of intermediate steps for documentation or further analysis. For statistical purposes users can analyze several data sets of experiments in one run by simply adding the data to a batch list.

Reducing complexity in multi-channel analysis
Samples with several fluorescent markers can be completely analyzed in one run by using separate analysis channels for different markers. Optionally, objects identified in different acquisition channels can be correlated, for example to count the number of DNA strand breaks per nucleus. 2D analysis can also be applied to color images - for example to determine the number of healthy cells in one analysis channel versus the number of abnormal cells in another analysis channel.

Control temperature, CO2 and O2, conditions during the experiment
Knowledge of all environmental parameters is crucial for researchers. The correct environmental conditions help minimize experiment failures and enable researchers to fully assess their results. With the Environmental Control module in LAS AF 4.0 users can monitor temperature, CO2 and O2 conditions at all times. They can also pre-define changes of these environmental parameters during an experiment and even let the system wait until a specified temperature is reached before starting. The range control either displays a warning if user-defined limits are exceeded or, alternatively, it stops the whole experiment. All environmental parameter controls are combined in one user-interface, making it easy to keep track. In combination with the LAS AF Live Data Mode module, acquisition macros can be defined to run temperature profiles - for example to perform heat shock experiments.

Monitoring experiments 24 / 7 - together with colleagues
On widefield systems, the LAS AF Mobile Connection module enables researchers to stay connected to and interact with their experiments 24 hours a day, seven days a week - without the need to stay close to the imaging system. Users can remotely start, stop or pause their experiments, capture images to identify the best time to start an experiment or display an image at a conference that has been taken seconds ago during a running experiment.

Mobile Connection works via web client or mobile device. As several users can be connected with the microscope system at the same time, they can also interact with each other, exchanging messages to discuss the most recent developments of a live experiment.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,400+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Yale University and Leica Microsystems Partner
Yale welcomes scientists to participate in core facility opening and super-resolution workshops October 20 through 31, 2014.
Tuesday, September 30, 2014
Scientific News
Potential “Good Fat” Biomarker
New method to measure the activity of energy consuming brown fat cells could ease the testing weight loss drugs.
MicroRNA Pathway Could Lead to New Avenues for Leukemia Treatment
Cancer researchers at the University of Cincinnati have found a particular signaling route in microRNA (miR-22) that could lead to targets for acute myeloid leukemia, the most common type of fast-growing cancer of the blood and bone marrow.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Doubling Down on Dengue
HMS researchers have discovered two ways a compound blocks dengue virus.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
AstraZeneca to Sequence 2 Million Genomes in Search for New Drugs
Company launches integrated genomics approach which aims to transform drug discovery and development.
Unique Model for Studying ALS
Unique mouse model will allow researchers to better study the genetic origins and potential treatments of ALS.
Targeting an ‘Undruggable’ Cancer Gene
RAS genes are mutated in more than 30 percent of human cancers and represent one of the most sought-after cancer targets for drug developers.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.
Improving Engineered T-Cell Cancer Treatment
Purdue University researchers may have figured out a way to call off a cancer cell assassin that sometimes goes rogue and assign it a larger tumor-specific "hit list."
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!