|A fast and fully automated solution for Lipidic Cubic Phase (LCP) screening using mosquito LCP|
Joby Jenkins1, Patricia Edwards2, Rob Lewis1and Joanne Franklin1
Membrane proteins, such as G-protein-coupled receptors, are known to be much more difficult to purify and crystallise than soluble proteins due to their native environment within the lipid bilayer of the cell membrane. The in meso (lipidic cubic phase or LCP) crystallisation technique has revolutionised the process of crystallising membrane proteins. This method utilises highly viscous lipid mesophases to contain the membrane proteins for crystallisation.
|A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3|
Andrew Goulter and Jason Mundin
Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.
|High-throughput imaging of cellular models using an Acumen eX3|
Paul Wylie, David Onley
The Acumen eX3 is the fastest imaging system available, collecting and simultaneously analysing over 40 images/second, covering the entire well, without the trade off of having to use lower resolution. Acumen is well established for cell-based high-content screening, but researchers have recently applied its large field of view to rapidly analyse complex cellular or animal models, such as angiogenic tube formation, C. elegans or drosophila larvae.
|A Mix-and-Read Cell-Based Assay for Antibody Screening Against Epidermal Growth Factor Receptor|
Wayne Bowen, David Onley, Tristan Cope
The conventional antibody screening assay based on antibody-antigen binding has been enzyme-linked immunosorbent assay (ELISA). While tedious and consuming, ELISA has proved sufficient for the identification of antibodies directed against secreted antigens. However, cell surface antigens (e.g. GPCRs) provide challenges for ELISA due to the shortage of soluble antigens and high variability resulting from loss of cells during wash procedures.
|Neutrophil Adhesion: A HCS Compatible Assay Using the Acumen eX3|
Diana Caracino and Paul Wylie
The Acumen eX3 can be used to study the process of cellular adhesion, whereby adherent cells types specifically, endothelial cells can be grown to confluence in microtitre plate wells and other cells types e.g. neutrophils added. The neutrophils can be differentially stained with calcein AM and the adhesion profile monitored and quantified. Cell adhesion can be determined simply by correlating retained fluorescence with cell number.
|The Future of Compound Management|
Dr Richard Kim, Ben Schenker, Simon Tullett
Current compound management practices have evolved to support both primary and secondary screening projects from a centralized repository storing a combination of plates and tubes. In this poster we will describe an innovative large-scale tube-based compound management approach to maximize lab space; improve compound stability; enable rapid generation of custom screening sets; while providing background QC and real time library integration of new chemical entities.
|An Integrated Solution for Automated Nanoliter Hit-Picking at BioFocus|
Joby Jenkins1, Dr Manuel Baader2 , Chloe Carter1, Stephen Starkie1
This poster describes a section of BioFocus’s screening workfolow where mosquito X1 is integrated with a RapidStak plate stacker (Thermo Scientific) using TTP LabTech’s CherryPicker software. This allows the mosquito X1 to work unattended for extended periods. The CherryPicker software drives the system automatically by converting pick lists provided by BioFocus’ LIMS system into mosquito protocols, and feeding appropriate plates via the RapidStak.
Chris de Graaf, Gerdien de Kloe, Henry Vischer, Mark Verheij, Saskia Nijmeijer, Azra Delic, David Maussang, Ken Chow, Anitha Shanmugham, Paul England, Rogier Smits, Rob Leurs and Iwan de Esch
A proprietary and structurally diverse fragment library has been created and screened for a variety of Gprotein coupled receptors (GPCRs) and a number of other drug targets. The data allows for a fragment-based chemogenomics study to interrogate the interactions of GPCRs and their ligands.
|Analytical and Chemical Knowledge Management Software for Drug Metabolism Science|
Graham A. McGibbon, Karim Kassam, and Susan Ling
Characterizing metabolite structures and metabolic pathways is essential to understanding the potential biological implications of compounds in drug discovery.