|Treatment of Highland Frogs from the Two Legged Stage with Homeopathically Prepared Thyroxin (10-11 – 10-21)|
Gerhard Lingg1, P. Christian Endler1,*, Michael Frass1, and Harald Lothaller2
For the experiment presented here, we used the same setup as for the initial multilaboratory study[1,2,3,4], but a special preparation process of the homeopathic drug involving dilution steps of 1: 50,000 and leading to thyroxin 10–11 – 10–21 was used.
|A high-throughput protein array-based approach |
Rachel Van Dyk, Claudia Kirisits, Paul Potter, Fook Tim Chew, Reinhard Hiller
Here we present a novel discovery-oriented high-throughput approach to the screening of allergen reactivates in crude biological extracts is presented using seafood allergens as an example. The CPGR workflow permits the effective screening of hundreds of putative allergens in parallel using minute amounts of patient serum and constitutes a cost-efficient allergen-specific antibody screening method for a routine diagnostic setting.
|3D-QSAR Common Feature Pharmacophore Model for Polyphenols as Potential Anti-Malarial Agents|
Shruti Saxena, Amit K. Gupta & Mridula Saxena
3D QSAR studies have been carried out on a series of polyphenols for their antimalarial activity using CATALYST program. Hypothesis with three features namely hydrophobic (1), hydrogen bond donor (1) and hydrogen bond acceptor (1) was found to the best, which mapped well with the most active and least active compound of the test set. This model can be used to develop drugs for malarial chemotherapy.
|A Novel Fluorimetric Assay for the Detection of TACE (a-Secretase)|
Vera Rakhmanova, Xudong Zhu, Fengying Li, Xiaofen Zhong, Anita Hong and Xiaohe Tong
To facilitate high throughput screening of TACE inhibitors, AnaSpec synthesized a novel peptide substrate for TACE using QXL™ 520/5-FAM FRET pair. Using this FRET substrate, AnaSpec developed a new kit – the SensoLyte™ 520 TACE Activity Assay. This kit can be used to detect the activity of the enzyme and for screening of TACE inhibitors. It is highly sensitive and can detect subnanogram amounts of enzyme.
|Purpose-Built LIMS for Life Sciences and High Throughput Screening Laboratories|
Thermo Fisher Scientific Inc. have announced the availability of a new technical poster demonstrating the importance of configurability and flexibility in laboratory information management systems (LIMS) design for life sciences and high throughput screening applications.
|High Content Image Analysis using a Laser Scanning Microplate Cytometer|
Paul Wylie, Ben Schenker, Sarah Payne and Andrew Goulter
Microscope-based high-content instruments offer high optical resolution, however, the limited field of view afforded by their objective lenses can mean lengthy read times for some assays, especially where multiple image capture per well is required. Wherever possible, only a small percentage of the total number of cells present in a test well are analysed to keep plate read times at a minimum, which may not always be ideal. Microplate laser- scanning cytometers, such as TTP LabTech’s Acumen® eX3
|A Modular Solution for Storage of Biological Samples|
Joby Jenkins, Wayne Bowen, Ben Schenker, Chloe Milburn
The physical and chemical composition of biological samples is more diverse than the libraries of medicinal compounds used for drug discovery research. Types of samples include proteins, nucleic acids, blood and serum samples, cell suspensions, tissue biopsies, and antibodies. Thus any storage system must be flexible to accommodate the differing storage requirements of each sample type base upon its biochemical composition, stability and where appropriate, solvent.
|Low Volume Liquid Handling of Organic Solvents for Compound Storage and Dissolution using mosquito|
Gillian Lewis, Tristan Cope, Joby Jenkins, David Gledhill & Rob Lewis
mosquito® is a positive displacement liquid handling system capable of pipetting solutions with a high degree of accuracy (to within 5%) and precision (CVs of <10%) in the nanolitre range. Here, we demonstrate mosquito’s ability to dispense accurately low volumes of organic solvents of low surface tension and to re-dissolve compounds which have been previously dried in storage wells.
|Improving the efficiency of 384 "mini-tube" technology using mosquito nanlolitre pipetting|
Joby Jenkins, Wayne Bowen, Rob Lewis & Chloe Milburn
384-well plate “mini-tube” consumables have been developed by automation companies such as REMP and The Automation Partnership (TAP) to hold compound samples divided into single-use storage tubes. Each tube holds 40 -75 µL, and is filled, sealed, stored and then thawed just once before use. However, when accessed by conventional pipetting technology, the tube’s practical working volume is as little as 20 µL, which means high compound wastage. The seal must also be pierced prior to pipetting to p