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Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Anne F Hammerstein, Diana Caracino, and Paul Wylie

The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.

Combining low and high volume liquid handling capabilities for ADME screening
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk

In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.

ADP-Glo™ Kinase Profiling Systems for Targeted and Flexible Kinase Inhibitor Profiling
Jacquelyn Hennek, Juliano Alves, Said Goueli, and Hicham ZegzoutiPromega Corporation, 2800 Woods Hollow Road, Madison, WI 53711

Drug safety is of paramount importance in the pharmaceutical industry indicating that minimal side effects constitute a major requirement in drug development.

Does the increase of exosomal microRNAs reflect an activated immune system in melanoma?
Nina Koliha, Florian S. Dreyer , Jochen Dindorf , Andreas Bosio, Andreas S. Baur , and Stefan Wild

Cutaneous malignant melanoma is a form of skin cancer that accounts for 65% of skin cancer-related deaths. The incidence increases continuously, and while early detection leads to nearly 100% survival rates, the mortality raises to greater than 80% for patients with advanced disease.

A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins
Antti Vasala, BioSilta Oy, Oulu, Finland

The ability to express and extract fully functional proteins in sufficient quantities from bacterial cultures is a prerequisite for many projects in which recombinant proteins are required for structural studies, functional characterization, as assay components or for other applications. However, low yield, poor solubility and lack of functionality are often associated with, what should be, a routine procedure.

Over-representation of Proteins Identified as Disease Biomarkers and their Relation to Post-Mortem Events
B. Orback, K. Kultima, M. Borén, M. Söderquist and K. Sköld

Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level resulting in loss of sample quality due to post sampling changes. It has recently been reported that the same proteins, regardless of tissue origin or species, are often found expressed differentially in various disease states, bringing into question the significance of these proteins as biomarkers.

Function of Protein Kinase C-1 in memory in Caenorhabditis elegans
Neil Patel, Shivon Manchanda, Aarohi Shah, Melissa McSweeney, and Aryeh Routtenberg

To study long lasting olfactory memory in C. elegans, we conditioned the nematodes to form an association between the odorant benzaldehyde and starvation.

Integrating compound storage into automated laboratory workflows
James Craven, Simon Tullett

This poster discusses the benefits of comPOUND for automated sample storage and delivery. TTP Labtech’s pneumatic transport technology, lab2lab, integrates these stores with other instrumentation into managed, fully automated workflows, allowing the scientist to focus on research and data analysis.

A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope

Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.

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Showing Results 111 - 120 of 355
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Soy Shows Promise as Natural Anti-Microbial Agent
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Doubling Down on Dengue
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Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
AstraZeneca to Sequence 2 Million Genomes in Search for New Drugs
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