|Microcarrier based production of cryopreserved cells|
Sharon Davies, Lynne Smith, Sian Kalinka, Philip Meyler, Chris Jones, Rahman Ismail, Elizabeth Price, Stephen Game, J M Kendall., GE Healthcare
The use of cellular assays in drug screening continues to grow with over 50% of primary screens using cell based formats in 2006. The majority of cells used in the drug discovery industry are fresh, using ‘just in time’ batch processing from in house facilities. This process could give rise to a number of issues, namely batch variation, scheduling of cell production and capacity management. We describe the use of microcarrier technology in combination with a suitably configured bioreactor to pro
|Smart Solutions in drug discovery|
Ian Kalinka, Lynne Smith, Elizabeth Price, Angela Marenghi, J.M.Kendall, Ray Ismai
Here we present data on the development of a large scale production process for the provision of two different cryopreserved transiently transfected cell lines. Included is identification of a suitable transfection reagent that is efficient, non-toxic and cost effective. This process has been validated using these two cell lines and two different cell-based assays.
|The application of microcarrier technology for the provision of receptor membranes for high-throughput screening|
Liz Price, Angela Marenghi, Gareth Ellis#, Stephen Game, Rahman Ismail, GE Healthcare
The recent growth in cell use for drug discovery, particularly for high-throughput screening (HTS), has posed a number of challenges including batch performance, cell production scheduling and capacity management in response to the increasing demands for cells and assay components derived from cells (e.g. membrane fractions).
|Quality Tools and their Application in the Production of Cellular Reagents for Screening Assays.|
Sharon Davies, Lynne Smith, Lisa Bailey, Martyn Birch, Rahman Ismail, Peter Tatnell, Michelle Doyle, Liz Price and J.M Kendall, GE Healthcare
The use of mammalian cell based assays in compound screening continues to grow. Over 50% of all screening assays (HTS and HCA) now utilise cells or cell derived reagents.
|Protein array-based screening of autoantibody signatures|
Zingaretti C., Arigò M., Colombatto P., Brunetto M., Muratori L., Bonino F., Bianchi F., Pagani M., De Francesco R., Abrignani S., Bombaci M.
The evidence for an association between autoimmune diseases and chronic HCV infection has been clearly established. To this aim, a protein array was employed to analyze serum samples of HCV patients w/wo autoimmune complications, of patients with autoimmune hepatitis but not infected with HCV and of healthy donors as controls. A panel of autoantigens able to discriminate among the three groups of patients was identified for potential use as biomarkers.
|Clinical Evaluation of human embryonic stem cells (hESCs) induced with directed differentiation to gonadotrope cells to cure vasculogenic impotency and to improve coital frequency in males. An open st|
Timothy S. Andersson, David K. Chin, Wendy Hiu Wai Wong
This work demonstrates the clinical efficacy, tolerability and safety of patient-syngenic hESC induced with directed differentiation to gonadotrope cells. Hypothalamus transmits gonadotropin releasing factor to pituitary that sets off LH and FSH to Sertoli cell and Seminiferous tubule resulting Leydig cell to produces testosterone. This potential offers a rationale to evaluate hESCs to cure patients with vasculogenic impotency and to improve coital frequency in males.
|Clinical Evaluation of induced pluripotent stem cells (iPSc) to cure patients with Psoriasis Vulgaris. An Open Study.|
Timothy S. Andersson, David K. Chin, Wendy H. Wong
To evaluate the clinical efficacy of iPSc directed to interfere T-cell activation to cure patients with psoriasis. Twenty subjects were enrolled. Each patient was administered iv with syngenic iPS. Those showing lower PASI score were considered cured. The study states that induced iPSCs are safe and impart significant clinical efficacy to cure patients with psoriasis.
|Software for Genomic/Epigenomic Research|
A.S.Tanas, V.V.Shkarupo, E.B.Kuznetsova, D.V.Zaletayev, V.V. Strelnikov
We present two computer programs: 1) AIMS in silico - a suggestion tool for Amplification of Intermethylated Sites experimental design and results analysis; and 2) PeakPick – a tool to view & analyse capillary electrophoregrams Our computer software is intended to standardize AIMS applications and to turn it into one of the principal approaches towards cancer methylomes studies as well as towards diagnostics in oncology, including early screening.
|Geneset-based cancer survival analysis: On the non-uniform distributiuon of p-values under the null hypothesis|
Esteban Czwan, Benedikt Brors, David Kipling
Although null p-values are assumed to be uniformly distributed in gene expression experiments, the actual distribution often deviates from the assumed distribution. This can incorrectly associate the biology of a geneset with cancer prognosis in geneset-based survival studies. To assess the implications of this, a geneset-based method was developed. This method empirically approximates the distribution of null p-values and tests whether predefined sets of biologically-related genes are associate