|Transporter-based In Vitro-In Vivo Extrapolation (IVIVE)|
Viera Lukacova, Michael B. Bolger, Walter S. Woltosz, Neil J. Parrott, Agnès Poirier and T. Lavè
Mechanistic analysis of in vitro measurements to obtain values of kinetic parameters for drug interactions with transporters, as well as for passive diffusion through cell membranes.
|ISMTR-283 Protects Human Bone Marrow Progenitors from the Lethal Effects of Ionizing Radiation|
Clarke E, Traynor-Kaplan A
Bone Marrow (BM) is extremely susceptible to radiation damage. BM from matched donors is often used to ‘rescue’ patients following medical radiation for leukemia. However, in the event of accidental/environmental radiation exposure, the ability to identify appropriate donors in a timely manner is rarely possible. Using CFC assays, we evaluated a small molecule compound (ISMTR-283) for its potential to protect the marrow progenitor cells and microenvironment from radiation damage.
|Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces|
Abernethy N, Chung E, Fontanelle BT, Gao Y, Jennins D, Koudijs MM, Lim D, Yang L, Ling T, Vukovic P, Wong A, Maeji, NJ
The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.
|UPLC-MS/MS for the Screening, Confirmation and Quantification of 32 Drugs Illegally Added to Herbal/Dietary Supplements for the Enhancement of Male Sexual Performance|
Salman Azimi, Nayan Mistry and Michelle Wood
This poster outlines development of a novel screening method that is suitable for both the detection of known and unknown ED drugs and analogues. Comprehensive spectral data is collected and automatically compared to a prepared library if known drugs.
|Effect of Liquid Handling Quality Control on Biological Assay Performance|
Nathaniel Hentz, Ph.D and Tanya Knaide
High quality assays are required in order to take forward potential drugs, post screening. Therefore the liquid handling must be accurate and precise, as demonstrated in this experiment.
|Nanoliter Volume Pin Tool Transfers as Measured by a Dual-Dye Absorbance Method|
Duong T. Chau; Patrick H. Cleveland, Ph.D.; John Thomas Bradshaw, Ph.D.
Increasing costs of chemical compounds and commonly used solvents has pushed high throughput screening labs towards lower working volumes, specifically in the nanoliter range. The ability to controllably dispense “known” nanoliter aliquots of samples is desired, which can readily be achieved using Pin Tools.
|Why Is My Assay Failing? An Approach to Assay Equipment Optimization|
Tanya R. Knaide, John Thomas Bradshaw, Kevin Khovananth, Keith Albert
Assays can produce unexpected or failing results for a multitude of reasons. Variability may be introduced at any point within the assay process.
|A Simple, Robust Automated Multiplexed Cell-Based Assay Process for the Assessment of Mitochondrial Dysfunction and Cytotoxicity|
Brad Larson, Peter Banks, Tracy Worzella, Andrew Niles and Timothy Moeller
Recent studies have shown that an increasing number of drugs no longer on the market have negative effects on mitochondrial function in key organs such as the liver and heart. Therefore it is increasingly important to monitor the effects of lead compounds on mitochondrial function in relevant cell systems. The ability to incorporate a simple, rapid, multiplexed, predictive assay can make the detection of potential toxic effects easier to perform early on in the drug discovery process.
|An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators|
Brad Larson, Bruce Sherf (INDIGO Biosciences), and Peter Banks
The Androgen Receptor (AR) is a member of the family of nuclear receptors responsive to steroid hormones. This poster aims to devise, validate and perform a preliminary automated HTS screening campaign to identify novel modulators of AR activity.