|A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins|
Antti Vasala, BioSilta Oy, Oulu, Finland
The ability to express and extract fully functional proteins in sufficient quantities from bacterial cultures is a prerequisite for many projects in which recombinant proteins are required for structural studies, functional characterization, as assay components or for other applications. However, low yield, poor solubility and lack of functionality are often associated with, what should be, a routine procedure.
|Over-representation of Proteins Identified as Disease Biomarkers and their Relation to Post-Mortem Events|
B. Orback, K. Kultima, M. Borén, M. Söderquist and K. Sköld
Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level resulting in loss of sample quality due to post sampling changes. It has recently been reported that the same proteins, regardless of
tissue origin or species, are often found expressed differentially in various disease states, bringing into question the significance of these proteins as biomarkers.
|Function of Protein Kinase C-1 in memory in Caenorhabditis elegans|
Neil Patel, Shivon Manchanda, Aarohi Shah, Melissa McSweeney, and Aryeh Routtenberg
To study long lasting olfactory memory in C. elegans, we conditioned the nematodes to form an association between the odorant benzaldehyde and starvation.
|Integrating compound storage into automated laboratory workflows |
James Craven, Simon Tullett
This poster discusses the benefits of comPOUND for automated sample storage and delivery. TTP Labtech’s pneumatic transport technology, lab2lab, integrates these stores with other instrumentation into managed, fully automated workflows, allowing the scientist to focus on research and data analysis.
|A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor |
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope
Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.
|Fast Liquid Differential Scanning Calorimetry (FLDSC)|
R. Splinter, A.W. van Herwaarden, A. Pfreundt, W.E. Svendsen, D. Istrate, W. van Eijk
Lysozyme experiments show that protein unfolding can be recorded at scan rates of up to 1000 °C/s, and for lysozyme concentrations of 1 % and probably even down to 0.1%.
|Digital Dispensing for Direct Dilution: New Flexibility in Dose-Response Analyses|
Jeff Nielsen, Ken Ward, Christie Dudenhoefer, David Ochs, Joshua Yu, and Kevin
In addition to the economic advantages of improving speed, precision, compound usage and waste, digital dispensing offers scientific advantages. This includes making the execution of complicated experiments easy and fast.
|Identification and Characterization of Strain Specific Drug Target by Subtractive Genome Analysis of Methicillin Resistance Staphylococcus aureus|
Kiran Saeed and Reaz Uddin
This study aimed to identify and characterize strain specific essential protein targets, which will eventually help in the identification of druggable proteins in two strains of MRSA by using different Bioinformatic tools and databases. We adopting a subtractive genome approach within the domain of Computational Biology.
|Low cost, low footprint, expandable automated biobanking solutions |
James Craven, Chris Morris, Maud Godfrey, Danielle Miller
This case study describes how TTP Labtech’s comPOUND storage modules have been employed by Abcam, a worldwide supplier of antibodies. Here, the turnaround time of sample placement and retrieval is an essential component for high quality service to its customers.