Corporate Banner
Satellite Banner
Technology Networks Header
Wednesday, October 01, 2014
Technology Networks
 
Register | Sign in
Home Page>Posters

  Posters

Resorufin - a lead for a new protein kinase CK2 inhibitor
Iben Skjøth Sandholt1, Birgitte Brinkmann Olsen1, Barbara Guerra1, Olaf-Georg Issinger1, Brigitte Boldyreff2

Screening of a natural compound library led to the identification of resorufin, as a highly selective and potent inhibitor for protein kinase CK2. Out of 52 kinases tested only CK2 was inhibited. The IC50 values determined for the CK2 holoenzymes were 1.5 µM and for the free catalytic subunits ca. 4 µM. In different cancer cell lines treatment with resorufin led to cell death and endogenous CK2 was inhibited.

Automation of cell proliferation assay sulforhodamine B fot the screening of stilbene derivatives
Anne Soikkeli, Cristina Sempio, Erkki Mannila, Arto Urtti, Jouni Hirvonen, Marjo Yliperttula

The SRB assay was implemented on a TECAN Genesis workstation by optimizing cell numbers and liquid handling parameters. Anticancer drugs vinblastine, doxorubicin and 5-fluorouracil were used for standardizing the assay with a DU-145 cell line. To screen the antiproliferative activities of a series of stilbene derivatives, altogether 121 different stilbene structures, a plate layout was designed on 96-well format.

Experimental Determination of ADMET Parameters in High Throughput, Using Colloidal Gold Composites and a Conductive Polymer as Reporting Reagents
Roberto Martinez-Neira, Patricia De Pril, Anne Van Hoonacker, Patrick Englebienne

We developed and validated a high throughput in vitro setting to experimentally determine hSA and Orosomucoid affinities, fraction absorbed, Log BBB and Redox potential of NCEs using gold nanoparticles functionalized with proteins, lipids or conductive polymers. Such automated ADME assays provide means for more objective decisions in early drug discovery.

High Throughput Anchorage-Independent Cell Colony Growth Assay with the IsoCyte™-HTS Platform
Steven C. Miller1, Charlyn Sarnecki2, Paul B. Comita1, and Evan F. Cromwell1

The advantages of anchorage-independent cell colony growth assays for drug development are well known. Monitoring proliferation of cells in colony form is more biologically relevant than as single cells and formation of 3-dimensional (3D) colonies in semi-solid media better represents in vivo environments.

Treatment of Plaque Infections and Periodontitis using Stem Cells- A New Prospect
Manoj Hariharan , Naveen Raj

Dental precursor cells are attractive for novel approaches to treat diseases like periodontitis, dental caries or to improve dental pulp healing and the regeneration of craniofacial bone and teeth. These cells are easily accessible and, in contrast to bone-marrow-derived mesenchymal stem cells, are more closely related to dental tissues. This paper gives a short overview of stem cells of dental origin.

A Novel Multiplexed Digital Gene Expression Technology
Gary K. Geiss1,#, Roger Bumgarner2, Brian Birditt1, Timothy Dahl1, Naeem Dowidar1, Dwayne L. Dunaway1, H. Perry Fell1, Sean Ferree1, Renee D. George1, Tammy Grogan1, Jeffrey J. James1, Malini Maysuria1, Jeffrey D. Mitton1, Paola Oliveri4, Jennifer L. Osborn3, Tao Peng2, Amber L. Ratcliffe1, Philippa J. Webster1, Eric H. Davidson4, and Leroy Hood5

We describe a novel platform, the nCounter Analysis System, for sensitive, highly multiplexed, digital gene expression analysis based on NanoString’s novel molecular barcoding technology. Detection of individual mRNA molecules using an assigned sequence of six different fluorescent spots per probe are detected, and then the number of times that code sequence appears in a sample are counted.

The Aetiological, Clinical and Metabolic Profile of Hypokalemic Periodic Paralysis in an Indian Population
Jubbin Jacob, Narsing Rao, Mathew John, Simon Rajaratnam , Nihal Thomas , M.S.Seshadri

Hypokalemic periodic paralysis (HPP) represents a heterogeneous group of disorders that presents with acute muscular weakness. Familial periodic paralysis is the commonest cause of hypokalemic periodic paralysis in Caucasians and thyrotoxic paralysis in the Orientals. In this analysis, we have complied the etiological factors that appear to be more dominant in Indian population

qPCRas a primary screen in drug discovery
GauravJaggi, Frank Boeckler, Andreas Joerger and Alan Fersht

We report the use of qPCR technique to follow the thermal unfolding of proteins by the binding of the dye SYPRO Orange, and exploit its potential as a robust and high-throughput primary screen for small molecule drug discovery.

Targeting Inflammatory Cytokines Using Adenoviruses: gene delivery of biological therapies in ovarian cancer
Michael A. Salako, Hagen Kulbe, Iain A. McNeish, Frances R. Balkwill

Constitutive TNF-alpha expression is characteristic of the malignant ovarian surface epithelium. Adenoviral mutants hold great promise as gene therapy vectors but their efficacy is hindered by an inflammatory cascade orchestrated by TNF-alpha. We found that delivering TNF-alpha shRNA to ovarian cancer cells using oncolytic adenoviruses could reduce the inflammatory cascade generated by adenoviruses and also had direct anti-tumour activity on the cancer cells.

<< 10 11 12 13 14 15 16 >>
Showing Results 121 - 130 of 267
Scientific News
UW-Madison Team Developing ‘Tissue Chip’ to Screen Neurological Toxins
A faster, more affordable way to screen for neural toxins.
Improving Korean Wheat
Dae Wook Kim learns genetic screening techniques to develop a line of Korean wheat.
Blood Test May Help Determine Psychosis Risk
A study led by University of North Carolina at Chapel Hill researchers represents an important step forward in the accurate diagnosis of people who are experiencing the earliest stages of psychosis.
Blood Test To Diagnose Depression
Test identifies nine blood markers tied to depression; predicts who will benefit from therapy.
Study Aims to Develop Breath Test for COPD
A new clinical iniative, lead by NYU Langone Medical Center, aims to determine a breath test’s effectiveness to identify volatile organic compounds (VOCs) in human breath that are biomarkers of chronic obstructive pulmonary disease (COPD).
In Directing Stem Cells, Context Matters
The surface cells are grown on has a profound effect on differentiation.
New Cell Model to Speed up Development of Brain Tumour Drugs
Rresearch looks set to improve screening for new cancer drugs and drug delivery systems specifically designed for children with brain tumours.
New Advances in the Pre-clinical Evaluation of Hepatitis B Therapies
Imperial College London to present first public HBV Data from CN Bio in vitro liver model.
Cancer-Fighting Drugs Might Also Stop Malaria Early
A number of compounds have been identified which could be used to fight malaria.
Bowel Disease Gene Discovery
Genetic changes that occur in patients with the bowel condition Crohn’s disease could hold clues to fighting the illness.
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv