|Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions|
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)
The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
|Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip|
Javier Sanchez-Martin1, Simon Krattinger1, Margarita Shatalina1,2, Thomas Wicker1 and Beat Keller1*
Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip
|Intact Protein LC-MS: How to Overcome the Challenges?|
Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart
Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.
|On-chip quantification of miRNA using digital droplet PCR|
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2
miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
|High-throughput analysis of protein formulations by DLS|
Sophia Kenrick and Daniel Some
High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1
|Digital microphotographic atlas of the adult zebrafish cerebellum|
Kristen Lee, Megan Landy, Ashton Cline, Christopher Corbo, and Zoltan Fulop
Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.
|The Effects of Dimethylphthalate on the Viability and Development of Drosophila melanogaster|
Sara Mfarrej, Dr. Heather Cook, Dr. Donald Stearns, Dr. Zolton Fulop
My research studies the effect of dimethylphthalate (DMP), a putative endocrine disruptor, on the viability and development of the model organism Drosophila melanogaster.
|Analytical Pipetting of Serum|
John Thomas Bradshaw, PhD, Leah Flumerfelt, Richard H. Curtis, PhD, Rachel Parshley
Many types of chemical and biological analyses are based upon analytical techniques involving accurate delivery of liquid components. Pipetting tools have become commonplace especially in many biological and pharmaceutical laboratories. While these tools are familiar to many, their operational differences when pipetting different types of solutions are often over-looked.
|Droplet Microfluidics for 3D Epithelial Cell Culture|
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan
The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.