|Side by Side: An Evaluation of 2D vs. 3D Cell Culture for High Throughput Screening in Drug Discovery|
Sophie Quick 1,2, Sinead Knight 1, Jon Winter 3
•3D cell culture has the potential to provide a more physiologically relevant model compared to standard tissue culture plastic.
•From a screening perspective the technology offers the possibility of more predictive drug responses but has an increased cost.
•The question: is it possible and, more importantly, is it worthwhile moving towards screening in High Throughput using a 3D model?
|High Throughput Screening in the European Lead Factory|
S.P. van Helden, W.H. Rutjes, C.A.A. van Boeckel and J.H.M. van den Broek
This paper describes workflows that have been implemented at the screening centre of the European Lead Factory and presents screening statistics on the first 18 months of operation.
|Use of a Microlitre Digital Liquid Handler for Screening Applications|
Joby Jenkins, Gillian Lewis, Wayne Bowen
Digital dispensing offers researchers the most freedom for experimental design and sample placement with each microplate. It makes it relatively simple to plan and execute the most desirable experimental design and not one predicated by manual or automated liquid handling.
|Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method|
Sarah Kessel, Eric Sincoff, Olivier Dery, Lori Fitton
3D Tumorspheroid models for improved predictivity in cancer drug discovery.
|Design and Evaluation of High Definition Probe for HPV genotyping Microarray|
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim
To improve the sensitivity and specificity of the HPV DNA Microarray, we adopted triple oligonucleotide probes for each targets and selected these probes not to have higher similarity of 75% with each others. These triple probes have shown 10 ~ 100 times higher sensitivities with comparable specificities than the conventional HPV DNA microarray of single oligonuclotide probe.
|Gut Microbial Metabolites and Hepatic Xenobiotic Metabolism: A High Throughput Screening Approach|
Glynn Martin, James Sidaway, Jonathan Swann
This poster highlights the combination of metabonomics and high throughput screening by the identification of gut microbial metabolites and a screening assay designed to determine their cytotoxicity to liver-like cell cultures.
|Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording|
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2
Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.
|Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells: High Throughput and High Content Assessment of Cardiac Toxicity and Drug Efficacy by Monitoring Cytosolic Free Calcium Transients|
1Kettenhofen R, 1Duenbostell A, 2Niedereichholz T, 3D’Angelo JM, 4Horai H, 5Schwengberg S, 1Bohlen H, 6Licher T"
Introduction of selected, pure human cardiomyocytes derived from induced pluripotent stem cell (hiPSCM) into a calcium transient imaging high throughput screening (HTS) assay to assess cardiotoxicity and drug efficacies.
|Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In Functional Assays|
Daniel Brown (1), Niklas Larsson (2), Ola Fjellström (3), Anders Johansson (3), Sara Lundqvist (2), Johan Brengdahl (2), and Richard J. Isaacs (1)
We describe the application of back-scattering interferometry (BSI) to the characterization of small molecule ligand binding to human GPR39 (a GPCR targeted for type-2 diabetes therapy) overexpressed in crude membrane fractions in free solution, including how BSI-derived affinity and functional assay-derived potency correlate for compounds of varying scaffolds.