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Monday, July 28, 2014
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Single Cell Analysis of Voltage-gated Potassium Channels for Electrophysiological Properties of Rat Hypothalamic Paraventricular Nucleus Neurons
Seul Ki Lee, Sora Lee, Seung Yub Shin, Pan Dong Ryu, So Yeong Lee

The hypothalamic paraventricular nucleus (PVN), an integrating site in the regulation of neuroendocrine and autonomic nervous systems, is composed of heterogeneous neurons, type I and type II, based on their electrophysiological properties. Single cell real-time RT-PCR analysis was made to investigate the molecular basis underlying the properties. The results suggest that Kv1.3, Kv4.2 and Kv4.3 potassium channels are the potential candidates in determining the electrophysiological properties in

Fragment Screening Using Capillary Electrophoresis
Simon Pettit, Carol Austin, Dallas Hughes, Sharon Magnolo, Leonid Raskovetsky

Capillary electrophoresis, which was pioneered for HTS in the 1990s, has recently been adapted to enable the screening of fragments. The technique is explained and the screening of fragments against a chaperone protein Hsp90 is presented as an example.

High-Throughput Multiplexed Inflammation Assay Using Primary Cells
Oksana Sirenko, Yen-Wen Chen, Jayne Hesley, Cathy Olsen, H. Roger Tang, Evan F. Cromwell*

An important event in the inflammation response is the expression of cell-surface antigens that facilitate binding of immune cells to blood vessels. The ability to monitor up-regulation of these molecules, such as VCAM, E-selectin, and HLADR on endothelial cells provides an important physiological read-out for cell-based models of inflammation.

Maximizing cell wash performance with the AquaMax® 2000/4000 microplate washers
Cathy Olsen, Iris Yang, and James Wasson

Many cell-based applications, including calcium flux assays, cell-based ELISAs, ion channel assays, and others require gentle but thorough washing of cells in order to ensure cell retention, cell integrity and optimal assay performance. The 96 cell wash head for AquaMax® 2000/4000 microplate washers from Molecular Devices uses angled pins to dispense fluid without disturbing cells. Adjustable dispense rate and aspiration height allow users to minimize disruption of even weakly adherent cells. We

Two secreted orthogonal bioluminescent reporters for non-destructive ultrasensitive assays
George Tzertzinis1, Salvatore Russello1, Cathy Olsen2

Here we present a powerful combination of two secreted bioluminescent reporters, the luciferases from Gaussia princeps and Cypridina noctiluca. We demonstrate that the properties of these two reporters in terms of sensitivity, compatibility and use in non- destructive assays conducted on live cells or supernatants from different well formats makes them suitable for novel high throughput format applications.

Label free technology: the weird and wonderful world of cell signaling
Ryall A†, Shearer J†, Brodbeck D*, Nufer O*, Cronk D† and Fasler S*

Whilst conducting an evaluation of label free technology platforms we encountered a number of surprising, but reproducible, observations. For example, receptor over-expression impacts endogenous signaling responses; surface coatings affecting cell adherence can change the response profile; and it is important to choose the appropriate analysis metric for complex profiles. Awareness of the subtleties of cell signaling within model systems such as CHO and HEK recombinant over-expression sys

Label free technology: value added information in the drug discovery process
Brodbeck D*, Nufer O*, Ryall A†, Shearer J†, Fasler S* and Cronk D†

We have evaluated Molecular Devices CellKey™ and SRU BIND® label free detection technologies and their application to hit finding and hit validation using cell based assays. The presented data highlights:

Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the POLARstar Omega.

The Oris™ Cell Migration Assays from Platypus offer certain advantages over traditional migration assays. Especially the TriCoated kits enable the researcher to test the effect of different extracellular matrix components like Collagen I or Fibronectin on migration. In this application note we show the real-time monitoring of cell migration using a POLARstar Omega from BMG LABTECH equipped with a CO2 gas vented system.

The Transcreener® ADP2 Universal Kinase Assay from BellBrook Labs is readily performed on BMG LABTECH microplate readers using different assay formats

The Transcreener® ADP2 FI assay kit from BellBrook Labs is a simple one-step competitive red fluorescence immunoassay based on the detection of ADP. In this application note we show that this assay is compatible with four different microplate readers from BMG LABTECH. With the PHERAstar FS and Plus, as well as the POLARstar and FLUOstar Omegas comparable standard curves and EC50 values were obtained.

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Showing Results 71 - 80 of 254
Scientific News
UK Government Must Do More to Reduce Risk of vCJD Infection
Report recommends that UK Government commission a full assessment of the risks currently faced by the UK blood supply.
NIST Instrument Enables High-speed Chemical Imaging of Tissues
Researchers have demonstrated a dramatically improved technique for analyzing biological cells and tissues based on characteristic molecular vibration "signatures."
Innovative Technology Enables First Crystal Structure of mGlu5 Transmembrane Domain to be Determined
Several novel drug candidates have been identified to help treat specific neuropsychiatric disorders.
Capturing Cancer: A Powerful New Technique for Early Diagnosis
Researchers describe an innovative technique for early disease detection, which they call immunosignaturing.
Significant Step Towards Blood Test for Alzheimer's
Scientists have identified a set of 10 proteins in the blood which can predict the onset of Alzheimer’s, marking a significant step towards developing a blood test for the disease.
Potential Alzheimer’s Drug Prevents Abnormal Blood Clots in the Brain
Researchers have identified a compound that might halt the progression of Alzheimer’s by interfering with the role of plaque forming amyloid-ß.
CRI Finds Key to Identifying, Enriching Mesenchymal Stem Cells
Biomarker enables researchers to accurately characterize the properties and function of MSCs in the body.
Finding Could Revolutionize Drug Discovery
A study by researchers at IRB Barcelona reveals the existence of information highways that connect and correlate distant sites within a single protein.
Advanced Cell Culture Techniques for Cancer Drug Discovery
This review focuses on the culturing of cell lines representative of solid cancers in advanced cell culture conditions.
Detailed Brain Imaging Study to Help Unlock Root Causes of Dementia
Large population scanning heralds a new era in research to tackle dementia and a wide range of intractable diseases.

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