|Efficacy of Gene Therapy in Restoring Vision in Leber's Congenital Amaurosis|
The research reviews current knowledge of the genetic causes of Leber's congenital amaurosis (LCA) and the development of a gene therapy intervention for LCA. The efficacy as well as the limitations of this form of treatment are evaluated in detail and recommendations are made for the future directions of gene therapy for Leber's congenital amaurosis.
|Recent Technical Developments in the Standardized Separation and Measurement of Extracellular Vesicles|
Amy Phillips (1), Robert Vogel(2), and Murray F. Broom(1)
We present robust and practical solutions for the standardization of EV isolation and concentration measurement. EV concentration also needs to have a defined size range. With these solutions, significant improvement in the reproducibility and comparability of results between different researchers is possible.
|High Titre BacMam viruses improve transduction efficiency of mammalian cells|
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King
We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.
|Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering|
Ilka Lewrenz, Stephanie Wälter, Jacqueline Franke
Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.
|Automated Fluorescence Detection and Imaging of RNA Species in Live Cells|
Paul Held, Victor Koong, Don Weldon, Peter Banks
This poster describes the detection and quantification of RNA species in Live cells through automated imaging.
|Identification and Characterisation of Novel Positive Allosteric Modulators of the Galanin 2 Receptor|
An HTS against GalR2 was carried out to find novel PAMs to treat neuropathic pain.
|The Longest Way Round is the Shortest Way Home: HTS Assay Development for Complex Multi-domain Protein Kinases|
Doris Hafenbrandl, Vanessa Nardese, Maria Cristina Sidoli, Valeria Wanke, Mariantonietta Rubino and Daniele Carettoni
This study strongly supports the possibility to overcome the major bottlenecks in the production of long kinase recombinant forms, in order to perform drug discovery programmes with proteins more closely preserving the structural and functional properties of the native enzymes.
|Enabling Epigenetics Studies from HTS to SAR : A Novel HTRF® Platform to Identify and Characterize Reader Domain Inhibitors|
T. Roux1, M. Badol1, N. Douayry1, L. Sergeant1, E.Trinquet1, F. Degorce1, S. Milhas2, S. Betzi2, C. Derviaux2, C. Eydoux3, J. Letienne2, A. Lugari2, Y. Collette2, J-C. Guillemot3 et X. Morelli2
Discover a novel HTRF platform to identify and characterize the vast variety of epigenetic binding domain.
|Artificial Multi-Gene Expression Systems Design Service for Natural Compound Formation and Hetero Protein Complexes|
Bernauer, Hubert, Gregor Zipf and Josef Maier
Drug discovery of natural compounds drug development and drug target analyses as well as bioproduction can benefit from artificial genetic systems and constructions. The direction in which genes are to be developed is written in the genomes. Synthesis oriented genomic analyses of codon bias and tRNA adaption analyses are prerequisites for generating adaptive, highly functional genes.