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Monday, September 15, 2014
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High-Throughput Multiplexed Inflammation Assay Using Primary Cells
Oksana Sirenko, Yen-Wen Chen, Jayne Hesley, Cathy Olsen, H. Roger Tang, Evan F. Cromwell*

An important event in the inflammation response is the expression of cell-surface antigens that facilitate binding of immune cells to blood vessels. The ability to monitor up-regulation of these molecules, such as VCAM, E-selectin, and HLADR on endothelial cells provides an important physiological read-out for cell-based models of inflammation.

Maximizing cell wash performance with the AquaMax® 2000/4000 microplate washers
Cathy Olsen, Iris Yang, and James Wasson

Many cell-based applications, including calcium flux assays, cell-based ELISAs, ion channel assays, and others require gentle but thorough washing of cells in order to ensure cell retention, cell integrity and optimal assay performance. The 96 cell wash head for AquaMax® 2000/4000 microplate washers from Molecular Devices uses angled pins to dispense fluid without disturbing cells. Adjustable dispense rate and aspiration height allow users to minimize disruption of even weakly adherent cells. We

Two secreted orthogonal bioluminescent reporters for non-destructive ultrasensitive assays
George Tzertzinis1, Salvatore Russello1, Cathy Olsen2

Here we present a powerful combination of two secreted bioluminescent reporters, the luciferases from Gaussia princeps and Cypridina noctiluca. We demonstrate that the properties of these two reporters in terms of sensitivity, compatibility and use in non- destructive assays conducted on live cells or supernatants from different well formats makes them suitable for novel high throughput format applications.

Label free technology: the weird and wonderful world of cell signaling
Ryall A†, Shearer J†, Brodbeck D*, Nufer O*, Cronk D† and Fasler S*

Whilst conducting an evaluation of label free technology platforms we encountered a number of surprising, but reproducible, observations. For example, receptor over-expression impacts endogenous signaling responses; surface coatings affecting cell adherence can change the response profile; and it is important to choose the appropriate analysis metric for complex profiles. Awareness of the subtleties of cell signaling within model systems such as CHO and HEK recombinant over-expression sys

Label free technology: value added information in the drug discovery process
Brodbeck D*, Nufer O*, Ryall A†, Shearer J†, Fasler S* and Cronk D†

We have evaluated Molecular Devices CellKey™ and SRU BIND® label free detection technologies and their application to hit finding and hit validation using cell based assays. The presented data highlights:

Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the POLARstar Omega.

The Oris™ Cell Migration Assays from Platypus offer certain advantages over traditional migration assays. Especially the TriCoated kits enable the researcher to test the effect of different extracellular matrix components like Collagen I or Fibronectin on migration. In this application note we show the real-time monitoring of cell migration using a POLARstar Omega from BMG LABTECH equipped with a CO2 gas vented system.

The Transcreener® ADP2 Universal Kinase Assay from BellBrook Labs is readily performed on BMG LABTECH microplate readers using different assay formats

The Transcreener® ADP2 FI assay kit from BellBrook Labs is a simple one-step competitive red fluorescence immunoassay based on the detection of ADP. In this application note we show that this assay is compatible with four different microplate readers from BMG LABTECH. With the PHERAstar FS and Plus, as well as the POLARstar and FLUOstar Omegas comparable standard curves and EC50 values were obtained.

A novel method for coupling capillary columns
Wil van Egmond, Daniela Peroni,

Using a proprietary, low-melting, deactivated glass we have developed a novel instant Meltfit™ connection. Reliable connectors are made in-situ in less than a minute.

A Comparison of AlphaLISA Bead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices
Anuradha Prasad, Ashleigh Price, Stephen Hurt, Rathnam Chaguturu

The University of Kansas in collaboration with PerkinElmer Inc. worked on looking at the comparison of the AlphaLISA Technology and an Electrochemiluminescence Technology to measure assay windows, lower and upper detection limits and intra - and inter-assay precision. In this study, three AlphaLISA no-wash assays, which employ a faster and less complex protocol, were found to deliver highly sensitive and accurate results, equivalent to those obtained in ECL technology.

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Showing Results 81 - 90 of 262
Scientific News
Study Aims to Develop Breath Test for COPD
A new clinical iniative, lead by NYU Langone Medical Center, aims to determine a breath test’s effectiveness to identify volatile organic compounds (VOCs) in human breath that are biomarkers of chronic obstructive pulmonary disease (COPD).
In Directing Stem Cells, Context Matters
The surface cells are grown on has a profound effect on differentiation.
New Cell Model to Speed up Development of Brain Tumour Drugs
Rresearch looks set to improve screening for new cancer drugs and drug delivery systems specifically designed for children with brain tumours.
New Advances in the Pre-clinical Evaluation of Hepatitis B Therapies
Imperial College London to present first public HBV Data from CN Bio in vitro liver model.
Cancer-Fighting Drugs Might Also Stop Malaria Early
A number of compounds have been identified which could be used to fight malaria.
Bowel Disease Gene Discovery
Genetic changes that occur in patients with the bowel condition Crohn’s disease could hold clues to fighting the illness.
Test Reliably Detects Inherited Immune Deficiency in Newborns
NIH-supported study suggests that early diagnosis of severe combined immunodeficiency leads to high survival rates.
Test Reliably Detects Inherited Immune Deficiency in Newborns
NIH-supported study suggests that early diagnosis of severe combined immunodeficiency leads to high survival rates.
Heart Molecule Discovery Could Lead to Effective Treatment for Heart Failure
Researchers have discovered a previously unknown cardiac molecule that could provide a key to treating, and preventing, heart failure.
UK Government Must Do More to Reduce Risk of vCJD Infection
Report recommends that UK Government commission a full assessment of the risks currently faced by the UK blood supply.

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