Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


SoPRano™: High Throughput and Sensitive Label-free Screening on a Plate Reader

Product Description

SoPRano™ is unique in enabling label-free SPR screening in solution using a standard absorbance plate reader, delivering rapid, cost effective, label-free assays for a broad range of applications:

•    Small molecule screening (including fragment screening);
•    Antibody screening and characterisation;
•    ADME and physico-chemical property determination.

particle+image.jpgSoPRano works by using specifically functionalized gold nanoparticles in solution (e.g. coated with a target receptor or antibody) which exhibit Localized Surface Plasmon Resonance (LSPR), a variant of the proven SPR phenomenon. With LSPR, plasmons remain localized on individual nanoparticles and induce an absorbance band in the visible spectrum that shifts with the refractive index of the medium.  This shift is readily quantifiable, such that when ligands interact with the functionalized nanoparticles they cause a reproducible absorbance change in the visible spectrum. As a result, SoPRano technology enables label-free screening on a standard absorbance plate reader.

SoPRano has many advantages that make it ideal for compound, fragment and antibody screening, as well as for high throughput ADME applications:

•    Solution-based ‘mix-and-read’ assays
•    96- and 384-well compatible
•    High sensitivity
•    Reproducible
•    Potential for high throughput
•    Requires only standard lab equipment – no expensive SPR instrument required!
•    Cost effective

In summary, SoPRano™ is a label-free, high throughput and highly flexible early drug discovery technology, that is broadly enabling, cost effective and requires no capital investment.

Build-your-own assay kits
SoPRano ‘build-your-own’ assay kits for small molecule and antibody applications are available, based upon a versatile and easy to use charge adsorption conjugation process:

•    CA-1 and CA-2 charge adsorption kits are available in 20, 100 and 500 ml sizes.

Additional coupling technologies are currently being developed.

The kits include colloidal gold nanoparticles and detailed protocols for conjugation and assay development, enabling you to couple your protein target or antibody and perform screens in your own labs. Kits are available in various sizes, from Starter Kits for preliminary assay development, through to large volume kits for higher throughput applications.

ADME and physico-chemical property kits
ADME and physic-chemical property kits are available for:
•    Human Serum Albumin (HSA)
•    Alpha-1-acid Glycoprotein (AGP)
•    HSA and AGP combination kit, with calculation of Fraction Unbound (Fu)
•    pKa
•    Redox Potential
•    Bioavailablity
•    Blood-Brain-Barrier

The ready to use ADME kits include pre-conjugated gold nanoparticles and detailed protocols.

Contract research services
Our highly experienced team also provides bespoke services for:
•    Assay development;
•    Screening;
•    ADME/physico-chemical assays

Product SoPRano™: High Throughput and Sensitive Label-free Screening on a Plate Reader
Company Pharma Diagnostics
Price Request a quote
More Information View company product page
Catalog Number Unspecified
Quantity Unspecified
Company Logo

Pharma Diagnostics
I&I Center Brussels Z.1 Researchpark 310 1731 Zellik Belgium



Email: enquiries@pharmadiagnostics.com



Scientific News
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
New Cancer Drug Target in Dual-Function Protein
Scientists at The Scripps Research Institute (TSRI) have identified a protein that launches cancer growth and appears to contribute to higher mortality in breast cancer patients.
Penn State, TB Alliance, and GSK Partner To Discover New Treatments For TB
A new collaboration between TB Alliance, GSK, and scientists in the Eberly College of Science seeks to find new small molecules that can be used to create antibiotics in the fight against tuberculosis (TB).
Molecular Map Provides Clues To Zinc-Related Diseases
Mapping the molecular structure where medicine goes to work is a crucial step toward drug discovery against deadly diseases.
Platelets are the Pathfinders for Leukocyte Extravasation During Inflammation
Findings from the study could help in the prevention and treatment of inflammatory pathologies.
Genetic Research Can Significantly Improve Drug Development
With drug development costs topping $1.2bn (£850 million) to get a single treatment to the point it can be sold and used in the clinic, could genetic analysis save hundreds of millions of dollars?
New Method Opens Door to Development of Many New Medicines
Findings from TSRI reveal human proteins are better drug targets than previously thought.
Diagnosing Systemic Infections Quickly, Reliably
Team develop rapid and specific diagnostic assay that could help physicians decide within an hour whether a patient has a systemic infection and should be hospitalized for aggressive intervention therapy.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Blood Test That Detects Early Alzheimer’s Disease
A research team, led by Dr. Robert Nagele from Rowan University School of Osteopathic Medicine and Durin Technologies, Inc., has announced the development of a blood test that leverages the body’s immune response system to detect an early stage of Alzheimer’s disease – referred to as the mild cognitive impairment (MCI) stage – with unparalleled accuracy.

SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!