Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


SoPRano™: High Throughput and Sensitive Label-free Screening on a Plate Reader

Product Description

SoPRano™ is unique in enabling label-free SPR screening in solution using a standard absorbance plate reader, delivering rapid, cost effective, label-free assays for a broad range of applications:

•    Small molecule screening (including fragment screening);
•    Antibody screening and characterisation;
•    ADME and physico-chemical property determination.

particle+image.jpgSoPRano works by using specifically functionalized gold nanoparticles in solution (e.g. coated with a target receptor or antibody) which exhibit Localized Surface Plasmon Resonance (LSPR), a variant of the proven SPR phenomenon. With LSPR, plasmons remain localized on individual nanoparticles and induce an absorbance band in the visible spectrum that shifts with the refractive index of the medium.  This shift is readily quantifiable, such that when ligands interact with the functionalized nanoparticles they cause a reproducible absorbance change in the visible spectrum. As a result, SoPRano technology enables label-free screening on a standard absorbance plate reader.

SoPRano has many advantages that make it ideal for compound, fragment and antibody screening, as well as for high throughput ADME applications:

•    Solution-based ‘mix-and-read’ assays
•    96- and 384-well compatible
•    High sensitivity
•    Reproducible
•    Potential for high throughput
•    Requires only standard lab equipment – no expensive SPR instrument required!
•    Cost effective

In summary, SoPRano™ is a label-free, high throughput and highly flexible early drug discovery technology, that is broadly enabling, cost effective and requires no capital investment.

Build-your-own assay kits
SoPRano ‘build-your-own’ assay kits for small molecule and antibody applications are available, based upon a versatile and easy to use charge adsorption conjugation process:

•    CA-1 and CA-2 charge adsorption kits are available in 20, 100 and 500 ml sizes.

Additional coupling technologies are currently being developed.

The kits include colloidal gold nanoparticles and detailed protocols for conjugation and assay development, enabling you to couple your protein target or antibody and perform screens in your own labs. Kits are available in various sizes, from Starter Kits for preliminary assay development, through to large volume kits for higher throughput applications.

ADME and physico-chemical property kits
ADME and physic-chemical property kits are available for:
•    Human Serum Albumin (HSA)
•    Alpha-1-acid Glycoprotein (AGP)
•    HSA and AGP combination kit, with calculation of Fraction Unbound (Fu)
•    pKa
•    Redox Potential
•    Bioavailablity
•    Blood-Brain-Barrier

The ready to use ADME kits include pre-conjugated gold nanoparticles and detailed protocols.

Contract research services
Our highly experienced team also provides bespoke services for:
•    Assay development;
•    Screening;
•    ADME/physico-chemical assays

Product SoPRano™: High Throughput and Sensitive Label-free Screening on a Plate Reader
Company Pharma Diagnostics
Price Request a quote
More Information View company product page
Catalog Number Unspecified
Quantity Unspecified
Company Logo

Pharma Diagnostics
I&I Center Brussels Z.1 Researchpark 310 1731 Zellik Belgium



Email: enquiries@pharmadiagnostics.com



Scientific News
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Charles River Acquires Agilux
Enhances Charles River’s early-stage capabilities in bioanalytical services.
Identified Compound Stops Cells Making Ribosomes
From study in yeast, researchers have identifed a compound that interferes with the assembly of ribosomes.
CES Score May Predict Response to Cancer Treatment
Researchers identify new type of biomarker that helps predict prognosis and response to several types of cancer treatment.
Treating Sepsis with Marine Mitochondria
Mitochondrial alternative oxidase from a marine animal combats bacterial sepsis.
New Therapeutic Target for Crohn’s Disease
A promising new target for drugs that treat IBD has been identified along with a possible biomarker for IBD severity.
Analysing 10,000 Cells Simultaneously
New techniquethat traps 10,000 cells on a single chip has potential for cancer screening for individuals.
Researchers Find Fungus-Fighting Compound
A compound has been identifed that blocks growth of a fungus responsible for lung infections and allergic reactions.
Peer Reviewed Study Demonstrates Mass Spec Technique
The peer reviewed study demonstrates MS workflow, TMTCalibrator workflow, which dramatically enhances detection of key early stage Alzheimer’s biomarkers.

SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!