"Experience the benefit of multiplexed, cell- and bead-based assays performed during high content screening in a high throughput screening environment, ensuring heterogeneous responses can be captured earlier in the research and discovery of new therapeutics. Now multiplexed, homogeneous, bead and cell-based assays become easy to develop and run as robust primary screens. The cytometer can scan a plate in as little as 3 minutes, regardless of cell- or well-density while resolving objects at micron resolution in four colors simultaneously. These fast scan times and whole well analysis can gather data from >150,000 wells per 8-hour day, achieving the acquisition rates expected of high-throughput screening. The cytometer's speed comes from unique design elements including a large depth of focus and large field of view which eliminates the requirements to periodically focus or acquire multiple exposures to cover an entire field of view. A light scatter (labe free) mode allows generation of data from non-fluorescent objects (e.g., stem cell colonies where addition of dyes is unacceptable).
To help simplify the workflow of moving suspension cytometry assays into the screening environment, the IsoCyte Cytometer leverages a microplate-based environment to streamline evaluation of both adherent and suspension cell types, eliminating separation steps for adherent cells or colonies while eradicating daily routine procedures to prime and clean fluid lines. This well-based environment utilizes small aliquots (384- or 1536-well plates), preserving valuable stem or primary cells. See the poster ""High-Throughput Multiplexed Assay for Analysis of Hematopoietic Stem Cells Differentiation and Hematopoietic Toxicity"". Additionally, a microplate based assay allows samples to be rescanned to confirm results or gather additional content at a higher magnification on other systems."
Charting Kidney Cancer Metabolism Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.Insights into the Function of the Main Class of Drug Targets About thirty percent of all medical drugs such as beta-blockers or antidepressants interact with certain types of cell surface proteins called G protein coupled receptors.Visualizing a Cancer Drug Target at Atomic Resolution Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.Honey’s Potential to Save Lives The healing powers of honey have been known for thousands of years. 3-D Printed Lifelike Liver Tissue for Drug Screening A team led by engineers at the University of California, San Diego has 3D-printed a tissue that closely mimics the human liver's sophisticated structure and function. The new model could be used for patient-specific drug screening and disease modeling. Cytoskeleton Crew Findings confirm sugar's role in helping cancers survive by changing cellular architecture.Biomarker for Recurring HPV-Linked Oropharyngeal Cancers A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University. Valvena, GSK Sign New R&D Collaboration Valneva to supply process development services for EB66® -based Influenza vaccines. Light Signals from Living Cells Fluorescent protein markers delivered under high pressure.Cellular 'Relief Valve' A team led by scientists at The Scripps Research Institute (TSRI) has solved a long-standing mystery in cell biology by showing essentially how a key “relief-valve” in cells does its job.