Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
Become a Member | Sign in
Home>Products>This Product

MaxDiscovery™ GAPDH ELISA Kit

Product Description
The MaxDiscovery™ Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) ELISA Kit is an enzyme immunoassay that analyzes the quantity of GAPDH in cells, tissues, serum or urine. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH initiates the second stage of glycolysis, catalyzing the reaction that converts glyceraldehyde 3-phosphate (GAP) into 1,3 bisphosphoglycerate (1,3 BPG). GAPDH oxidizes and phosphorylates GAP to produce 1,3 BPG, which is then used as an intermediate in the synthesis of ATP. While the glycolytic function of GAPDH is widely known, recent evidence suggests that GAPDH is a highly versatile molecule that plays several diverse roles in living systems. Mammalian GAPDH is involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. There have also been many findings that GAPDH plays a role in different pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases, i.e. Alzheimer’s and Huntington’s disease. The GAPDH gene is constitutively expressed at high levels in almost all tissues. However, the molecular mechanism that sustains high-level expression of this housekeeping enzyme is still unclear. GAPDH is almost always a tetramer and is localized to the cytoplasm in healthy cells. Translocation of GAPDH into the nucleus is seen during its role in the early stages of apoptosis and oxidative stress. Because of its high-level and constitutive expression, GAPDH is widely used as a loading control for Northern/Western blots and for protein normalization. Like most ELISA assays, the MaxDiscovery™ GAPDH ELISA Test relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of GAPDH protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm. Selected Citations: Jones, J. A. et al. (July, 2010) Alterations in membrane type-1 matrix metalloproteinase abundance after the induction of thoracic aortic aneurysm in a murine model. Am J Physiol Heart Circ Physiol, 299: H114 - H124. doi: 10.1152/ajpheart.00028.2010
Product MaxDiscovery™ GAPDH ELISA Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1 x 96 wells
Quantity 510
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122

Scientific News
Potential Target for Treatment of Autism
Grant of $2.4 million will support further research.
Sniffing Out Cancer
Scientists have been exploring new ways to “smell” signs of cancer by analyzing what’s in patients’ breath.
Inroads Against Leukemia
Potential for halting disease in molecule isolated from sea sponges.
Molecular ‘Kiss Of Death’ Flags Pathogens For Destruction
Researchers have discovered that our bodies mark pathogen-containing vacuoles for destruction by using a molecule called ubiquitin, commonly known as the "kiss of death."
A New Single-Molecule Tool to Observe Enzymes at Work
A team of scientists at the University of Washington and the biotechnology company Illumina have created an innovative tool to directly detect the delicate, single-molecule interactions between DNA and enzymatic proteins.
Milestone Single-Biomolecule Imaging Technique May Advance Drug Design
The first nanometer resolved image of individual tobacco mosaic virions shows the potential of low-energy electron holography for imaging biomolecules at a single particle level; a milestone in structural biology and a potential new tool for drug design.
Multi-Gene Test Enables Some Breast Cancer Patients to Safely Avoid Chemotherapy
A major study is providing the best evidence to date that a 21-gene test done on the tumor can identify breast cancer patients who can safely avoid chemotherapy.
Antidepressants Plus Blood-Thinners Slow Down Brain Cancer
EPFL scientists have found that combining antidepressants with anticoagulants slows down brain tumors (gliomas) in mice.
Diagnostics Breakthrough Brings Viral Sequencing to Doctors’ Toolkit
New screening tool produces up to 10,000-fold improvement in viral matches compared with traditional high-throughput methods.
New Virus Identified In Blood Supply
Scientists have discovered a new virus that can be transmitted through the blood supply.


Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos