Based on HTRF technology, the HTRF Transcreener ADP assay is a universal method for identifying and characterizing the phosphotransferase activity induced by any ATP/ADP dependent target, such as serine/threonine and tyrosine kinases, as well as lipid kinases and chaperonins.
The formation of ADP is detected by a specific monoclonal antibody labeled with Eu3+ cryptate, and directly correlates with the amount of phosphorylated substrate in kinase assays.
Features • Monoclonal antibody specific to ADP • Homogeneous non-radioactive screening alternative • Enzyme-free detection technology • Compatible with any substrate
Applications • Kinases and ATPases • High Throughput Screening • Profiling • Mechanistic studies
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