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Fluorescence-Based Measurement of Endothelial Cell Migration Through Collagen Using the BD Falcon FluoroBlok Insert System
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Molecular Devices

Cell motility data from advanced time-lapse videomicroscopy and dynamic confocal reconstruction techniques of cell migration through 3-D extracellular matrices suggest that the inhibition of matrix-degrading enzymes by pharmacological inhibitors may induce a conversion in tumor cell invasion strategy from proteolytic invasion to non-proteolytic ameboid crawling.1 This observation raises the question whether proteolysis of the extracellular matrix is a critical parameter for invasion and metastasis. The ability to measure the rate of proteolytic versus non-proteolytic mediated invasion of the extracellular matrix potentially reveals the relative contribution of matrix remodeling to invasion.

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