Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Automated Patch Clamping Trends
Learn more about current practices, preferences and metrics in ion channel drug screening using APC technology.
Mechanisms of Calcium Blockers
Researchers describe how the fundamental mode of action of two distinct chemical classes of calcium channel blockers differs.
Breakthrough in GPCR Understanding
Integral Molecular announces breakthrough in understanding the functionality of GPCRs, the largest class of drug targets in human disease.
Enzyme that Triggers Cell Demise in ALS Identified
Scientists from Harvard have identified a key instigator of nerve cell damage in people with amyotrophic lateral sclerosis (ALS).
Fly Study into Therapies for HVP-Induced Cancer
Fruit flies may help scientists understand the mechanism by which HPV can cause cancer as well as identify potential drug treatments.
World's Most In-Depth Study to Detect Alzheimer's Disease
A multisite team will see the most thorough and vigorous testing for Alzheimer's ever performed on volunteers.
'Missing Evolutionary Link' of Natural Drug Source Found
Scripps Florida study finds 'missing evolutionary link' of a widely used natural drug source
Grad Student's Finding Enables Rapid Compound Screening
Grad student makes technical leap that could enable rapid screening of anti-cancer compounds.
Uncovering Constructor Proteins
Scientists have discovered a new bacterial cell wall builder that could be a target for antibiotic development.
Cholesterol Linked to Aggressive Cancers
Research suggests fats, such as cholesterol, can increase aggressive cancer progression.
Scroll Up
Scroll Down

An AlphaScreen® SureFire® Phospho-ERK1/2 Assay Performed on BMG LABTECH’s POLARstar Omega
Bookmark and Share

BMG LABTECH

On activation GPCRs signal through different G-proteins (e.g. Gs, Gi or Gq) to elicit a cellular response which can be mediated through the activation of a number of different signaling cascades which include intracellular Ca2+ mobilization and the formation or inhibition of adenylate cyclase. Although these technologies have been successful in many cases they have had their limitation. Responses for many GPCRs such as Gi coupled GPCRs are not easily measured in current assay formats and other methods have been investigated as alternative readout. One such pathway is the ERK phosphorylation cascade, which results in the phosphorylation of both cytoplasmic and nuclear proteins regulating gene transcription. Hence this type of assay has the ability to measure cellular changes activated by Gs, Gi and Gq coupled receptors giving potential to use a single assay format for screening.

This application note details how the POLARstar Omega is used to detect ERK phosphorylation using a AlphaScreen® SureFire® proximity-based assay. ERK phosphorylation is demonstrated at receptors which signal via Gi and Gq G-proteins.  

Further Information

Related Content

25 Years of Revolutionary Microplate Reader Technology at the LS2
BMG LABTECH exhibits at the biggest Swiss Life Sciences Meeting for the first time.
Tuesday, December 16, 2014
BMG LABTECH Announces First FP User Group Meeting
The meeting brings together a wide range of speakers with extensive experience of Fluorescence Polarization techniques.
Monday, April 26, 2010
BMG LABTECH Announces First FP User Group Meeting
Company announces their inaugural UK Fluorescence Polarisation user group meeting on the 23rd of April 2010.
Monday, April 19, 2010
BMG LABTECH, World Leader in Microplate Reader Technology, is proud to be a Bronze Sponsor of the SBS Conference 2010.
Visit us at booth 817 and join us at our workshops to see latest innovations in microplate reading technology including the ultimate HTS Microplate Reader, PHERAstar FS
Tuesday, March 30, 2010
BMG Labtech Joins ALSSA
The ALSSA - Analytical & Life Science Systems Association - Board of Directors recently approved applications for membership submitted by BMG LABTECH, Inc. Based in Cary, North Carolina.
Thursday, March 25, 2010
DLReady Certification for the Omega Series of Microplate Readers
DLReady certification, awarded by Promega Corp., validates an instrument that has passed the strict performance levels and standards required for the Dual-Luciferase Reporter (DLR) assay system.
Monday, December 03, 2007
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!