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Growth of Campylobacter using BMG LABTECH’s FLUOstar Omega equipped with ACU
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Campylobacter jejuni was only properly linked with disease in the 1970’s when new culture methodologies were developed. C. jejuni has quite stringent requirements for growth, namely; a rich nutrient broth, temperatures of 37-42oC, and microaerophilic conditions, i.e. oxygen reduced to 5-8% and carbon dioxide elevated to 10%.

Historically in our laboratory Campylobacter jejuni growth studies have been performed in shaking liquid cultures in 15 mL tubes maintained at 42oC with 7% O2 and 10% CO2 in a variable atmosphere incubator (VAIN) cabinet; these are then removed temporarily from the incubator to take optical density readings at regular intervals. However this method has several limitations.

This application note describes a comparison of C. jejuni growth studies performed using the traditional method of tubes shaking in a VAIN with that of a study completed in a 96 well microplate format using a BMG LABTECH FLUOstar Omega plate reader equipped with the Atmospheric Control Unit (ACU) to independently control oxygen and carbon dioxide.  

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