Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Liquid Biopsies: Utilization of Circulating Biomarkers for Minimally Invasive Diagnostics Development
Market Trends in Biofluid-based Liquid Biopsies: Deploying Circulating Biomarkers in the Clinic. Enal Razvi, Ph.D., Managing Director, Select Biosciences, Inc.
Lab-on-a-Chip Offers Promise for TB and Asthma Patients
A device to mix liquids using ultrasonics is the first and most difficult component in a miniaturized system for low-cost analysis of sputum from patients with pulmonary diseases such as tuberculosis and asthma.
Protein Related to Long Term Traumatic Brain Injury Complications Discovered
NIH-study shows protein found at higher levels in military members who have suffered multiple TBIs.
Urine Proteins Point to Early-Stage Pancreatic Cancer
A combination of three proteins found at high levels in urine can accurately detect early-stage pancreatic cancer, researchers at the BCI have shown.
Researcher Discovers Trigger of Deadly Melanoma
New research sheds light on the precise trigger that causes melanoma cancer cells to transform from non-invasive cells to invasive killer agents, pinpointing the precise place in the process where "traveling" cancer turns lethal.
Crystal Clear Images Uncover Secrets of Hormone Receptors
NIH researchers gain better understanding of how neuropeptide hormones trigger chemical reactions in cells.
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Access Denied: Leukemia Thwarted by Cutting Off Link to Environmental Support
A new study reveals a protein’s critical – and previously unknown -- role in the development and progression of acute myeloid leukemia (AML), a fast-growing and extremely difficult-to-treat blood cancer.
Scroll Up
Scroll Down

Analysis of a New High Throughput Screening Detection Technology for Rapid hERG Safety Testing using a Fluorescence Polarization Assay
Bookmark and Share

Biotek Instruments

Background
Speed and sensitivity are the hallmarks of Fluorescence Polarization (FP) assay technologies. Due to this FP has seen penetration in the drug discovery and drug development markets for High Throughput Screening applications. For a good overview of applications/target audiences for FP here is an easy to read and interesting White Paper with good citations for further reading: Fluorescence Polarization in Life Sciences. The Predictor hERG biochemical FP assay has been used as a model of FP performance validation used on our Synergy lines, not only because it is an assay designed for HTS run at low volume in 384-well plates, but because quantification of hERG channel binding and inhibition are cardio toxicity tests recommended for all drug compounds before human use (e.g. in pre-clinical trials). Therefore, the assay needs to prove it can compare with GLP-compliant gold standard methods, and the detection technology used to produce that data must be rigorous enough to do the same. Although BioTek has published a number of pieces of marketing collateral using the Predictor hERG assay, until Synergy Neo, other Synergy models could only provide sensitivity acceptable for detection of this assay at the cost of speed. Publication of optimal read parameters was either largely ignored or minimized in the other BioTek collateral for this reason, as the combination of assay and detection parameters required for optimal data were largely prohibitory for HTS applications when competitors could do it at ~ 2 min plate read time within a 2 hour assay incubation window.

Further Information

Related Content

BioTek Expands in the United Kingdom
Company expands their ongoing investment with the opening of their new UK Office in Swindon.
Friday, June 05, 2015
BioTek Nominated for Department of Defense’s Top Employer Award
BioTek Instruments been nominated for the 2015 Secretary of Defense Employer Support Freedom Award by the Employer Support of the Guard and Reserve (ESGR), a Department of Defense (DoD) office.
Tuesday, March 31, 2015
BioTek Receives 2014 Supplier of the Year Award from Fisher Scientific
BioTek was presented with the award at Thermo Fisher Scientific’s annual meeting in Phoenix.
Wednesday, March 25, 2015
BioTek Receives Two Awards from ESGR
Company has received the Patriot Award and the Above and Beyond Award.
Friday, August 22, 2014
BioTek Receives Extraordinary New Product Line Award from Fisher
Company receives award for Cytation 3 Cell Imaging Multi-Mode Reader.
Tuesday, March 04, 2014
BioTek Announces Establishment of Japanese Office
BioTek Japan will be fully functional by July 1, 2013.
Monday, June 10, 2013
BioTek Responds to Growing AP Market Needs with New Taiwan Office
New office will focus on increasing BioTek's brand presence in Taiwan.
Tuesday, May 14, 2013
BioTek Wins Governor's Award for Outstanding Workplace Safety
BioTek receives the award by Governor Peter Shumlin at the 9th Annual Vermont Workplace Safety Conference.
Thursday, April 18, 2013
BioTek Germany Certified in Accordance with EN ISO 13485
Certification guarantees that all of BioTek’s products and services satisfy the requirements and comply with the currently valid legislation.
Monday, December 17, 2012
BioTek Receives European Patent for Microplate Washer Technology
Ultrasonic Advantage uses unique, integrated ultrasonic cleaning to reduce protein and salt crystal build-up.
Tuesday, June 12, 2012
BioTek Expands Asia Pacific Office
BioTek Instruments, Inc., continues their commitment to the Asia Pacific region with the expansion of their Singapore office.
Thursday, May 10, 2012
BioTek Sparkles as Diamond Sponsor at SLAS 2012
Exhibition to be held February 4-8, 2012 at the San Diego Convention Center.
Monday, January 30, 2012
BioTek Inducted into Deane C. Davis Vermont Business Hall of Fame
BioTek President/CEO Briar Alpert and Vice President Adam Alpert jointly accepted the award on behalf of BioTek.
Tuesday, June 14, 2011
BioTek Instruments Expands into Switzerland
BioTek Instruments announces the establishment of its newest European direct sales and service organization.
Wednesday, January 27, 2010
BioTek Instruments and Global Cell Solutions set to Collaborate
BioTek Instruments, Inc., and Global Cell Solutions, Inc., (GCS) announce a formal collaboration for the coming year at LabAutomation 2010. The poster Automation of a Microplate Cell-based Assay to Measure Activity of the Histamine H1 G Protein-coupled Receptor Using a Novel 3-D Cell Culture Technique is the first in a series of collaborative publications detailing the use of BioTek’s microplate instrumentation for various cell-based assays.
Wednesday, January 27, 2010
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!