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SUMO FRET-based assays performed on BMG LABTECH’s NOVOstar
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BMG LABTECH

The small ubiquitin-like modifiers SUMO1 and SUMO2 and their processing by the protease SenP1 was investigated by using an in vitro FRET assay performed on BMG LABTECH´s NOVOstar. The described assay has applications in SUMO protease characterization, enzyme kinetic analysis and high-throughput inhibitor screening.

The NOVOstar is the ideal platform to perform this FRET-based SUMO assay, because it has a unique pipetting system that can add very small amounts of substance (<1 µl) followed by instantaneous reading.

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