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First siRNA Library Screening in Hard-to-Transfect HUVEC and Jurkat Cells
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Amaxa GMBH

High-throughput transfection of siRNA libraries has become a valuable tool in target identification and validation. However, such screenings have so far been constrained to mostly easy- to-transfect adherent cell lines. amaxa’s Nucleofector® 96-well Shuttle® System extends these approaches to primary and hard-to-transfect cells. In this application note we report results of two different screening approaches in hard-to-transfect cell types using Thermo Scientific Dharmacon siARRAY® siRNA libraries.

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