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High-Throughput Nucleofection® of Primary Rat Hippocampal Neurons
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Amaxa GMBH

Postmitotic neurons are routinely difficult to transfect. This application note describes the efficient, high-throughput Transfection® of plasmid DNA into primary hippocampal neurons. Using amaxa’s Nucleofector® 96-well Shuttle® System, 30 to 50 % of neurons were successfully transfected and showed normal neuronal development. In addition, quantitative down-regulation of target proteins by RNA interference was achieved.

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amaxa to Co-ordinate Collaboration to Develop High Throughput Devices for Primary Cell Transfection
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