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The Nucleofector® 96-well Shuttle® System – Nucleofection® of Cell Lines and Primary Cells in an Automated Framework
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Amaxa GMBH

Screening of siRNA or cDNA libraries requires an automated high-throughput transfection process. Besides mere throughput considerations, the main drivers for automation are the standardization and robustness of the process. Stability of all cell parameters is the key to statistically relevant data and valid results. Therefore we analyzed the stability of transfection results of different cell types during a time span typically needed to screen a focused siRNA library using Nucleofection®. The transfection results generated demonstrate the robustness of the automated Nucleofection® process.

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