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 | Regulation of antibody immunotherapy by fine-tuning FcR interactions Mark Cragg, Southampton University School of Medicine, speaking at Stem Cells & Bioprocessing 2012. |
 | Immunogenicity Assessment for the Successful Development of Biosimilars Dr Michael Tovey, Institut Andre Lwoff/INSERM, speaking at Stem Cells & Bioprocessing 2012. |
Benchtop Automation Trends 2013
HTStec
This market report summarizes the results of HTStec's industry-wide global web-based benchmarking survey on small-scale or benchtop automation carried out in January 2013.
ELISA Assay Trends
HTStec
This market report summarizes the results of HTStec’s 2nd industry-wide global web-based benchmarking survey on enzyme-linked immunosorbent assays (ELISA) carried out in June 2012.
Live Cell Kinetic Imaging Trends
HTStec
This market report summarizes the results of HTStec’s 2nd global web-based benchmarking survey on live cell kinetic imaging assays and instruments carried out in May 2012.
Life Science and Chemical Instrumentation Market (2011 - 2016)
MarketsandMarkets
Spectroscopy, Chromatography, DNA Sequencer & Amplifier, Lab Automation, Array, Flow Cytometer, Electrophoresis, Immuno Assay & Others
Single Cell Omics 2011
Select Biosciences Ltd
The single cell Omics revolution is the potential transformation of cellular heterogeneity from a source of noise to a source of new, as yet hidden discoveries.
Cancer Targeted Therapies and Immunotherapies Market Forecast
Datamonitor plc
The oncology market has witnessed a sizeable shift of focus away from cytotoxic therapies and antihormonal therapies towards the more lucrative targeted therapies and immunotherapies class in the last decade. This class boasts several of the top-selling anticancer brands overall, and has fueled most of the recent rapid growth of the oncology market as a whole.
In Vitro Diagnostics (Immunochemistry and Infectious Immunology) Global Market Briefing to 2016
GlobalData
This report provides key data, information and analysis on the global immuno chemistry and infectious immunology markets. The report provides market landscape, competitive landscape and market trends information the immuno chemistry and infectious immunology markets. The report provides comprehensive information on the key trends affecting the market, and key analytical content on the market dynamics. The report also reviews the competitive landscape and technology offerings.
Monoclonal Antibodies: 2010
Datamonitor plc
Across the branded prescription pharmaceutical market monoclonal antibodies (MAbs) are forecast to be the strongest performing molecule type, delivering a forecast six year compound annual growth rate of 9.5% over 2009-15, outpacing the growth rates of small molecules, therapeutic proteins and vaccines.
Manufacturing of Biologics - Increasing Demand for Monoclonal Antibodies and Recombinant Proteins Drives Increases in Capacities and Innovative Production Technologies
GBI Research
GBI Research's report, “Manufacturing of Biologics - Increasing Demand for Monoclonal Antibodies and Recombinant Proteins Drives Increases in Capacities and Innovative Production Technologies”, provides the key data and analyzes the major challenges and drivers for the biomanufacturing industry. The report also analyzes the strengths, weaknesses of and opportunities for the stakeholders in the manufacturing of biologics.
Competitor Analysis Biosimilar and Biobetter/Biosuperior Antibodies
La Merie
The present Competitive Intelligence Report about Biosimilar and Biobetter/Biosuperior Antibodies provides a competitor evaluation in the field of recombinant monoclonal and polyclonal antibodies directed against CD20, Her2, VEGF, TNF, EGF-R, RSV and GPIIb/IIIa as of October 2010.
HTStec
This market report summarizes the results of HTStec's industry-wide global web-based benchmarking survey on small-scale or benchtop automation carried out in January 2013.
ELISA Assay Trends
HTStec
This market report summarizes the results of HTStec’s 2nd industry-wide global web-based benchmarking survey on enzyme-linked immunosorbent assays (ELISA) carried out in June 2012.
Live Cell Kinetic Imaging Trends
HTStec
This market report summarizes the results of HTStec’s 2nd global web-based benchmarking survey on live cell kinetic imaging assays and instruments carried out in May 2012.
Life Science and Chemical Instrumentation Market (2011 - 2016)
MarketsandMarkets
Spectroscopy, Chromatography, DNA Sequencer & Amplifier, Lab Automation, Array, Flow Cytometer, Electrophoresis, Immuno Assay & Others
Single Cell Omics 2011
Select Biosciences Ltd
The single cell Omics revolution is the potential transformation of cellular heterogeneity from a source of noise to a source of new, as yet hidden discoveries.
Cancer Targeted Therapies and Immunotherapies Market Forecast
Datamonitor plc
The oncology market has witnessed a sizeable shift of focus away from cytotoxic therapies and antihormonal therapies towards the more lucrative targeted therapies and immunotherapies class in the last decade. This class boasts several of the top-selling anticancer brands overall, and has fueled most of the recent rapid growth of the oncology market as a whole.
In Vitro Diagnostics (Immunochemistry and Infectious Immunology) Global Market Briefing to 2016
GlobalData
This report provides key data, information and analysis on the global immuno chemistry and infectious immunology markets. The report provides market landscape, competitive landscape and market trends information the immuno chemistry and infectious immunology markets. The report provides comprehensive information on the key trends affecting the market, and key analytical content on the market dynamics. The report also reviews the competitive landscape and technology offerings.
Monoclonal Antibodies: 2010
Datamonitor plc
Across the branded prescription pharmaceutical market monoclonal antibodies (MAbs) are forecast to be the strongest performing molecule type, delivering a forecast six year compound annual growth rate of 9.5% over 2009-15, outpacing the growth rates of small molecules, therapeutic proteins and vaccines.
Manufacturing of Biologics - Increasing Demand for Monoclonal Antibodies and Recombinant Proteins Drives Increases in Capacities and Innovative Production Technologies
GBI Research
GBI Research's report, “Manufacturing of Biologics - Increasing Demand for Monoclonal Antibodies and Recombinant Proteins Drives Increases in Capacities and Innovative Production Technologies”, provides the key data and analyzes the major challenges and drivers for the biomanufacturing industry. The report also analyzes the strengths, weaknesses of and opportunities for the stakeholders in the manufacturing of biologics.
Competitor Analysis Biosimilar and Biobetter/Biosuperior Antibodies
La Merie
The present Competitive Intelligence Report about Biosimilar and Biobetter/Biosuperior Antibodies provides a competitor evaluation in the field of recombinant monoclonal and polyclonal antibodies directed against CD20, Her2, VEGF, TNF, EGF-R, RSV and GPIIb/IIIa as of October 2010.
Nuvo Research Announces Scientific Presentation at the Annual Meeting of AAI
Data evaluating WF10's therapeutic impact on signs and symptoms of experimentally induced rheumatoid arthritis in mice.
Yale Cancer Center Carves New Path in Immunotherapy
Cancer immunotherapy is showing promise in treating patients with a variety of advanced, metastatic tumors.
UCSF Scientists Use Human Stem Cells to Generate Immune System in Mice
Raising hopes for cell-based therapies, UC San Francisco researchers have created the first functioning human thymus tissue from embryonic stem cells in the laboratory.
Hormone May Help Treat Diabetes
Betatrophin prompts cells in the pancreas to multiply and produce more insulin.
Newly Described Type of Immune Cell and T Cells Share Similar Path to Maturity
Better understanding of cells' development has implications in study of inflammatory diseases.
Women’s Immune Systems Remain Younger for Longer
The slower decline in a woman’s immune system may contribute to women living longer than men.
Tumor-Activated Protein Promotes Cancer Spread
Researchers report that cancers physically alter cells in the lymphatic system to promote the spread of disease.
Cobra Biologics and Vaccibody Sign Agreement to Manufacture DNA Vaccine
Companies announce agreement to manufacture Vaccibody’s lead DNA vaccine candidate against HPV-induced malignancies.
Your Immune System: On Surveillance in the War Against Cancer
Wake Forest Baptist Research looks at gene expression profiling in breast cancer.
NIH Scientists Create New Tool for Identifying Powerful HIV Antibodies
It is believed that this latest advance could speed HIV vaccine research.
Data evaluating WF10's therapeutic impact on signs and symptoms of experimentally induced rheumatoid arthritis in mice.
Yale Cancer Center Carves New Path in Immunotherapy
Cancer immunotherapy is showing promise in treating patients with a variety of advanced, metastatic tumors.
UCSF Scientists Use Human Stem Cells to Generate Immune System in Mice
Raising hopes for cell-based therapies, UC San Francisco researchers have created the first functioning human thymus tissue from embryonic stem cells in the laboratory.
Hormone May Help Treat Diabetes
Betatrophin prompts cells in the pancreas to multiply and produce more insulin.
Newly Described Type of Immune Cell and T Cells Share Similar Path to Maturity
Better understanding of cells' development has implications in study of inflammatory diseases.
Women’s Immune Systems Remain Younger for Longer
The slower decline in a woman’s immune system may contribute to women living longer than men.
Tumor-Activated Protein Promotes Cancer Spread
Researchers report that cancers physically alter cells in the lymphatic system to promote the spread of disease.
Cobra Biologics and Vaccibody Sign Agreement to Manufacture DNA Vaccine
Companies announce agreement to manufacture Vaccibody’s lead DNA vaccine candidate against HPV-induced malignancies.
Your Immune System: On Surveillance in the War Against Cancer
Wake Forest Baptist Research looks at gene expression profiling in breast cancer.
NIH Scientists Create New Tool for Identifying Powerful HIV Antibodies
It is believed that this latest advance could speed HIV vaccine research.
Curie-Cancer and Roche Strengthen Partnership
Curie-Cancer and Roche build on four years partnership by increasing translational research programs, speeding up development of new cancer treatments.
ImmunoCellular Therapeutics Announces Issuance of Key Patent for Cancer Vaccine Target
The US Patent and Trademark Office (USPTO) has issued a patent covering methods of use of interleukin 13-receptor alpha 2 protein (IL-13Ra2) in cancer immunotherapy.
New Roche Award for Antibody-based Cancer Therapy Research
Researchers from academia throughout Europe are invited to participate.
MorphoSys Initiates Phase 2 Study of MOR208 in NHL
Company has dosed the first patient in Phase 2 clinical trial of anti-CD19 antibody.
MorphoSys Announces First Scientific Publication on Novel Antibody Platform Ylanthia
Detailed design, generation and testing of the Ylanthia library.
Seattle Genetics Reports First Quarter 2013 Financial Results
Total revenues of $57.3 million, including $33.9 million in ADCETRIS® (Brentuximab Vedotin) net product sales.
Enzo and NIH File Patent Application for Autoimmune Diseases
Collaborators filed a patent application for the management of autoimmune diseases that may include age-related macular degeneration (AMD), which is a leading cause of blindness in the United States.
Baxter Announces Topline Results of Phase III Study of Immunoglobulin for Alzheimer's
Phase III clinical study of immunoglobulin (IG) did not meet its co-primary endpoints of reducing cognitive decline and preserving functional abilities in patients with mild to moderate Alzheimer’s disease.
Harlan Laboratories Continues Global Growth
Harlan Laboratories announced the expansion of its specialty research production facilities in Indianapolis and Livermore, CA.
GBI and Coldstream Laboratories Announce a Collaboration
Collaboration to develop and manufacture cytotoxic Antibody Drug Conjugates.
Curie-Cancer and Roche build on four years partnership by increasing translational research programs, speeding up development of new cancer treatments.
ImmunoCellular Therapeutics Announces Issuance of Key Patent for Cancer Vaccine Target
The US Patent and Trademark Office (USPTO) has issued a patent covering methods of use of interleukin 13-receptor alpha 2 protein (IL-13Ra2) in cancer immunotherapy.
New Roche Award for Antibody-based Cancer Therapy Research
Researchers from academia throughout Europe are invited to participate.
MorphoSys Initiates Phase 2 Study of MOR208 in NHL
Company has dosed the first patient in Phase 2 clinical trial of anti-CD19 antibody.
MorphoSys Announces First Scientific Publication on Novel Antibody Platform Ylanthia
Detailed design, generation and testing of the Ylanthia library.
Seattle Genetics Reports First Quarter 2013 Financial Results
Total revenues of $57.3 million, including $33.9 million in ADCETRIS® (Brentuximab Vedotin) net product sales.
Enzo and NIH File Patent Application for Autoimmune Diseases
Collaborators filed a patent application for the management of autoimmune diseases that may include age-related macular degeneration (AMD), which is a leading cause of blindness in the United States.
Baxter Announces Topline Results of Phase III Study of Immunoglobulin for Alzheimer's
Phase III clinical study of immunoglobulin (IG) did not meet its co-primary endpoints of reducing cognitive decline and preserving functional abilities in patients with mild to moderate Alzheimer’s disease.
Harlan Laboratories Continues Global Growth
Harlan Laboratories announced the expansion of its specialty research production facilities in Indianapolis and Livermore, CA.
GBI and Coldstream Laboratories Announce a Collaboration
Collaboration to develop and manufacture cytotoxic Antibody Drug Conjugates.
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Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation
Functional Genetics
Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.
Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces
Anteo Diagnostics
The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.
Automated Solutions for Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cellular Cytotoxicity Utility
BioTek Instruments
Since the end of the 1990’s, the pharmaceutical industry has seen an increased interest in biologics, especially in the therapeutic areas of oncology and inflammation. Here we present the automation of two assays for the characterization and selection of potent antibody drug candidates. Both assays rely on HTRF® detection. The first assay quantifies the binding affinity of antibodies to their target antigen, on live cells.
Improving Cell-Mediated Cytotoxicity Assessment through the Use of an Automated Luminescent ADCC Assay
BioTek Instruments
Assays that can assess the ability of a biosimilar to act in a manner similar to the original biologic have seen increased interest. This poster describes the use of a non-radioactive luminescent chemistry to simplify the assay process and provide improved data quality.
Modular Bench Top Automation
BioTek Instruments
ELISA is one the most utilized assay formats in biomedical research. Numerous clinical, veterinary, and research assays use the specificity of antibodies to identify a diverse array of analytes from any number of different matrices. This poster details a modular bench top workstation capable of automating the assay process steps of most conventional ELISA assays.
Optical Microchip Sensors for Multiplexed Detection of Biological Pathogens
Stratophase
SpectroSens, a multi-channel optical microchip sensor system suitable for rapid, label-free multiplexed detection of a wide range of bio-hazardous agents is presented. Optical chips containing multiple high-precision planar Bragg gratings are exploited as low-cost, robust refractive index sensors.
A Mix-and-Read Cell-Based Assay for Antibody Screening Against Epidermal Growth Factor Receptor
TTP Labtech Ltd
The conventional antibody screening assay based on antibody-antigen binding has been enzyme-linked immunosorbent assay (ELISA). While tedious and consuming, ELISA has proved sufficient for the identification of antibodies directed against secreted antigens. However, cell surface antigens (e.g. GPCRs) provide challenges for ELISA due to the shortage of soluble antigens and high variability resulting from loss of cells during wash procedures.
Gene expression analysis of CD14+ monocytes immunomagnetically separated directly from whole blood: adaptation of protocols towards clinical trial requirements
1Miltenyi Biotec GmbH
Peripheral blood is widely used as starting material for biomarker discovery and validation using molecular biology technologies. The vast majority of currently published transcriptome data is based on RNA derived either from stabilized whole blood or peripheral blood mononuclear cells (PBMCs). Here, gene expression profiling studies and SOPs for fast, easy and specific manual or automated isolation of monocytes directly from whole blood are being described.
In silico screening of new potential TCR/CollagenII-MHCII inhibitors against rheumatoid arthritis
National Research Council (CNR) and Catholic University of Rome
Residues 261-273 of type II collagen bound to the MHC class II allele HLA-DR4 play a crucial role in rheumatoid arthritis. The protein–protein interactions between TCR and CII-MHCII complex may therefore serve as targets for the development of new drugs against RA. The aim of this study is to develop a pharmacophore virtual screening followed by molecular docking and dynamics calculations leading to the identification of new TCR/CII-MHCII inhibitors.
New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells
Biomedical Engineering Dept. and Dept. of Medicine &Epidemiology of Vet. Med. of UC Davis
We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue
Functional Genetics
Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.
Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces
Anteo Diagnostics
The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.
Automated Solutions for Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cellular Cytotoxicity Utility
BioTek Instruments
Since the end of the 1990’s, the pharmaceutical industry has seen an increased interest in biologics, especially in the therapeutic areas of oncology and inflammation. Here we present the automation of two assays for the characterization and selection of potent antibody drug candidates. Both assays rely on HTRF® detection. The first assay quantifies the binding affinity of antibodies to their target antigen, on live cells.
Improving Cell-Mediated Cytotoxicity Assessment through the Use of an Automated Luminescent ADCC Assay
BioTek Instruments
Assays that can assess the ability of a biosimilar to act in a manner similar to the original biologic have seen increased interest. This poster describes the use of a non-radioactive luminescent chemistry to simplify the assay process and provide improved data quality.
Modular Bench Top Automation
BioTek Instruments
ELISA is one the most utilized assay formats in biomedical research. Numerous clinical, veterinary, and research assays use the specificity of antibodies to identify a diverse array of analytes from any number of different matrices. This poster details a modular bench top workstation capable of automating the assay process steps of most conventional ELISA assays.
Optical Microchip Sensors for Multiplexed Detection of Biological Pathogens
Stratophase
SpectroSens, a multi-channel optical microchip sensor system suitable for rapid, label-free multiplexed detection of a wide range of bio-hazardous agents is presented. Optical chips containing multiple high-precision planar Bragg gratings are exploited as low-cost, robust refractive index sensors.
A Mix-and-Read Cell-Based Assay for Antibody Screening Against Epidermal Growth Factor Receptor
TTP Labtech Ltd
The conventional antibody screening assay based on antibody-antigen binding has been enzyme-linked immunosorbent assay (ELISA). While tedious and consuming, ELISA has proved sufficient for the identification of antibodies directed against secreted antigens. However, cell surface antigens (e.g. GPCRs) provide challenges for ELISA due to the shortage of soluble antigens and high variability resulting from loss of cells during wash procedures.
Gene expression analysis of CD14+ monocytes immunomagnetically separated directly from whole blood: adaptation of protocols towards clinical trial requirements
1Miltenyi Biotec GmbH
Peripheral blood is widely used as starting material for biomarker discovery and validation using molecular biology technologies. The vast majority of currently published transcriptome data is based on RNA derived either from stabilized whole blood or peripheral blood mononuclear cells (PBMCs). Here, gene expression profiling studies and SOPs for fast, easy and specific manual or automated isolation of monocytes directly from whole blood are being described.
In silico screening of new potential TCR/CollagenII-MHCII inhibitors against rheumatoid arthritis
National Research Council (CNR) and Catholic University of Rome
Residues 261-273 of type II collagen bound to the MHC class II allele HLA-DR4 play a crucial role in rheumatoid arthritis. The protein–protein interactions between TCR and CII-MHCII complex may therefore serve as targets for the development of new drugs against RA. The aim of this study is to develop a pharmacophore virtual screening followed by molecular docking and dynamics calculations leading to the identification of new TCR/CII-MHCII inhibitors.
New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells
Biomedical Engineering Dept. and Dept. of Medicine &Epidemiology of Vet. Med. of UC Davis
We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue
Direct Visualisation, Sizing and Counting of Aggregation in Proteins
NanoSight Ltd
Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
Automated High Throughput Functional Cell-based Bioassays for EGFR Blocking Antibodies
BioTek
This study describes a novel, target-specific, cell-based assay platform that can be used to detect and differentiate potential inhibitors of Epidermal Growth Factor receptors.
Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System
Molecular Devices
Accurate measurement of the concentration of a biological product is important in bioreactor/fermenter monitoring, downstream processing and final product quality control. Currently, electrophoretic, chromatographic and immunochemical methods are the most commonly used. These three methods are currently used in combination to provide the desired sample information.
Optimizing the Labeling of Proteins
Molecular Devices
The Threshold® Immuno-Ligand Assay (ILA) adapts reagents used in ELISA, RIA or radioreceptor assays for the Threshold System by covalently attaching biotin or fluorescein labels to the binding proteins and/or ligands. To simplify labeling and purification of proteins, Molecular Devices has formulated biotin and fluorescein as N-hydroxysuccinimide esters.
The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH
BMG LABTECH
ELISAONE ™ technology has been developed by TGR BioSciences to provide a means of running high performance sandwich immunoassays in a user-friendly 96-well format.
Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules
BMG Labtech
During manufacture of antibody molecules, they are subjected to mechanical stress generated by processes such as pumping and filtration. This may cause denaturation and consequently aggregation due to exposure of the protein to air-liquid interfaces and shear forces, resulting in the ultimate loss in bioactivity.
Quantitative Determination of Total Bile Acids with a Thermo Scientific Varioskan Flash Multimode Reader Using a Kinetic Enzyme Cycling Assay
Thermo Fisher Scientific
Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.
LanthaScreen TR-FRET Assay Performance with Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader
Thermo Fisher Scientific
LanthaScreen technology uses time-resolved fluorescence resonance energy transfer (TR-FRET) to study multiple target classes including protein kinases, nuclear hormone receptors, as well as proteases and ubiquitinated proteins.
Pipetting performance of Thermo Scientific Finnpipette F2 in combination with Thermo Scientific Finntip Flex
Thermo Fisher Scientific
The Finnpipette® F2 product family has been designed by taking into account the demands of today´s laboratory work and the require¬ments of the end users. It offers superior comfort, performance, reliability and repeatability in one of the lightest pipettes available.
Ergonomics and Usability Study of Thermo Scientific Finnpipette F1 and F2 Pipettes
Thermo Fisher Scientific
The objective of the study was to evaluate the ergonomics and usability of six manual, singlechannel pipettes. This was done by measuring the load and strain symptoms exhibited by workers’ muscles and joints, and evaluating the usability features of pipettes in a simulated work situation.
NanoSight Ltd
Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
Automated High Throughput Functional Cell-based Bioassays for EGFR Blocking Antibodies
BioTek
This study describes a novel, target-specific, cell-based assay platform that can be used to detect and differentiate potential inhibitors of Epidermal Growth Factor receptors.
Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System
Molecular Devices
Accurate measurement of the concentration of a biological product is important in bioreactor/fermenter monitoring, downstream processing and final product quality control. Currently, electrophoretic, chromatographic and immunochemical methods are the most commonly used. These three methods are currently used in combination to provide the desired sample information.
Optimizing the Labeling of Proteins
Molecular Devices
The Threshold® Immuno-Ligand Assay (ILA) adapts reagents used in ELISA, RIA or radioreceptor assays for the Threshold System by covalently attaching biotin or fluorescein labels to the binding proteins and/or ligands. To simplify labeling and purification of proteins, Molecular Devices has formulated biotin and fluorescein as N-hydroxysuccinimide esters.
The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH
BMG LABTECH
ELISAONE ™ technology has been developed by TGR BioSciences to provide a means of running high performance sandwich immunoassays in a user-friendly 96-well format.
Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules
BMG Labtech
During manufacture of antibody molecules, they are subjected to mechanical stress generated by processes such as pumping and filtration. This may cause denaturation and consequently aggregation due to exposure of the protein to air-liquid interfaces and shear forces, resulting in the ultimate loss in bioactivity.
Quantitative Determination of Total Bile Acids with a Thermo Scientific Varioskan Flash Multimode Reader Using a Kinetic Enzyme Cycling Assay
Thermo Fisher Scientific
Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.
LanthaScreen TR-FRET Assay Performance with Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader
Thermo Fisher Scientific
LanthaScreen technology uses time-resolved fluorescence resonance energy transfer (TR-FRET) to study multiple target classes including protein kinases, nuclear hormone receptors, as well as proteases and ubiquitinated proteins.
Pipetting performance of Thermo Scientific Finnpipette F2 in combination with Thermo Scientific Finntip Flex
Thermo Fisher Scientific
The Finnpipette® F2 product family has been designed by taking into account the demands of today´s laboratory work and the require¬ments of the end users. It offers superior comfort, performance, reliability and repeatability in one of the lightest pipettes available.
Ergonomics and Usability Study of Thermo Scientific Finnpipette F1 and F2 Pipettes
Thermo Fisher Scientific
The objective of the study was to evaluate the ergonomics and usability of six manual, singlechannel pipettes. This was done by measuring the load and strain symptoms exhibited by workers’ muscles and joints, and evaluating the usability features of pipettes in a simulated work situation.
