Corporate Banner
Satellite Banner
Immunology
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease

Published: Thursday, March 22, 2012
Last Updated: Thursday, March 22, 2012
Bookmark and Share
The main goal of the current work was to investigate kinetically alterations in parasitemia and leukocytes of the peripheral blood, cardiac inflammation and the cytokine profile of T-cell subsets in the spleen during the acute-phase of experimental infection by MT forms.

Abstract
Although metacyclic and blood trypomastigotes are completely functional in relation to parasite-host interaction and/or target cell invasion, they differ in the molecules present on the surface. Thus, aspects related to the variability that the forms of T. cruzi interacts with host cells may lead to fundamental implications on the immune response against this parasite and, consequently, the clinical evolution of Chagas disease. We have shown that BT infected mice presented higher levels of parasitemia during all the acute phase of infection. Moreover, the infection with either MT or BT forms resulted in increased levels of total leukocytes, monocytes and lymphocytes, specifically later for MT and earlier for BT. The infection with BT forms presented earlier production of proinflammatory cytokine TNF-a and later of IFN-c by both T cells subpopulations. This event was accompanied by an early cardiac inflammation with an exacerbation of this process at the end of the acute phase. On the other hand, infection with MT forms result in an early production of IFN-c, with subsequent control in the production of this cytokine by IL-10, which provided to these animals an immunomodulatory profile in the end of the acute phase. These results are in agreement with what was found for cardiac inflammation where animals infected with MT forms showed intense cardiac inflammation later at infection, with a decrease in the same at the end of this phase. In summary, our findings emphasize the importance of taking into account the inoculums source of T. cruzi, since vectorial or transfusional routes of T. cruzi infection may trigger distinct parasite-host interactions during the acute phase that may influence relevant biological aspects of chronic Chagas disease.

This iarticle is published online in PLoS ONE and is free to access.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Separation of mAbs Molecular Variants by Analytical HIC-HPLC
HIC-HPLC is a less widely used analytical technique compared with standard methods for separating variants based on size, charge or electrophoretic mobility.
Thursday, June 05, 2014
Constrained Evolution Drives Limited Influenza Diversity
Looking at the reasons behind the surprisingly low rate of H3N2 influenza A virus evolution.
Thursday, May 24, 2012
Algae-Produced Surface Protein Elicits Antibodies That Inhibit Malaria Transmission
In this study, scientists tested whether algal chloroplasts can produce malaria transmission blocking vaccine candidates, Plasmodium falciparum surface protein 25 (Pfs25) and 28 (Pfs28).
Thursday, May 24, 2012
Functional Metabolomics Reveals Novel Active Products in the DHA Metabolome
In this review, researchers from Harvard Medical School provide an update and overview of functional metabolomics that identified a new bioactive metabolome of docosahexaenoic acid.
Monday, May 14, 2012
The Immune Response to Melanoma is Limited by Thymic Selection of Self-Antigens
This study emphasizes the importance of investigating thymic expression of self-antigens prior to their inclusion in vaccination and immunotherapy strategies.
Wednesday, April 18, 2012
Quantitative Proteomics Reveals that only a Subset of the Endoplasmic Reticulum Contributes to the Phagosome
Early proteomics studies indicated that the endoplasmic reticulum (ER) might contribute to phagosome genesis. This article provides compelling evidence of ER recruitment to phagosome by biochemical and morphological approaches.
Tuesday, March 27, 2012
M cell Targeting by a Claudin 4 Targeting Peptide Can Enhance Mucosal IgA Responses
This study tests the recently developed Claudin 4 targeting peptide in it's ability to deliver vaccines via the uptake of nanoparticles through nasal M cells.
Thursday, March 22, 2012
Profiling of the BRCA1 Transcriptome Through Microarray and ChIP-chip Analysis
Data from the study suggests a model, whereby BRCA1 is present on defined promoters as part of an inactive complex poised to respond to various genotoxic stimuli.
Wednesday, October 19, 2011
Towards Protein Crystallization as a Process Step in Downstream Processing of Therapeutic Antibodies: Screening and Optimization at Microbatch Scale
Researchers from Biberach University of Applied Sciences demonstrate that crystallization has the potential to be included in downstream processing as a low-cost purification or formulation step.
Monday, October 17, 2011
High-Efficiency Screening of Monoclonal Antibodies for Membrane Protein Crystallography
Researchers from Brandeis University present an optimized process for efficient screening from immunization to final validation of monoclonal antibody for membrane protein crystallography.
Friday, September 23, 2011
NMR, Biophysical and Biochemical Studies Reveal the Minimal Calmodulin-Binding Domain of the HIV-1 Matrix Protein
Research from the University of Alabama may assist in the identification of the functional role of Calmodulin-Gag interactions in the HIV replication cycle.
Thursday, September 01, 2011
Profiling the Humoral Immune Response of Acute and Chronic Q Fever by Protein Microarray
To assess the antibody repertoire of acute and chronic Q fever patients researchers from the University of California have constructed a protein microarray containing the causative agent of Q fever.
Wednesday, August 31, 2011
Effects of Different Centrifugation Conditions on Clinical Chemistry and Immunology Test Results
Centrifugation time has a considerable impact on turn-around-time. This article studies the effect of centrifugation time of heparinized blood samples on clinical chemistry and immunology.
Tuesday, August 09, 2011
Cell Chip Array for Microfluidic Proteomics Enabling Rapid in situ Assessment of Intracellular Protein Phosphorylation
This article, published in Biomicrofluidics, discusses the ability to perform fluorescent immunocytochemistry, following cell fixation, using a microfluidic array of primary, nonadherent, single CD34+ stem cells.
Wednesday, June 22, 2011
Revisiting the Technical Validation of Tumour Biomarker Assays: How to Open a Pandora's Box
This article discusses the challenges for the technical validation of immunohistochemical and gene expression assays to detect tumour biomarkers and provides suggestions of pragmatic solutions to address these challenges.
Tuesday, May 03, 2011
Scientific News
Food Triggers Creation of Regulatory T Cells
IBS researchers document how normal diet establishes immune tolerance conditions in the small intestine.
Therapeutic Approach Gives Hope for Multiple Myeloma
A new therapeutic approach tested by a team from Maisonneuve-Rosemont Hospital (CIUSSS-EST, Montreal) and the University of Montreal gives promising results for the treatment of multiple myeloma, a cancer of the bone marrow currently considered incurable with conventional chemotherapy and for which the average life expectancy is about 6 or 7 years.
Cellular 'Relief Valve'
A team led by scientists at The Scripps Research Institute (TSRI) has solved a long-standing mystery in cell biology by showing essentially how a key “relief-valve” in cells does its job.
Switch Lets Salmonella Fight, Evade Immune System
Researchers at the University of Illinois at Chicago have discovered a molecular regulator that allows salmonella bacteria to switch from actively causing disease to lurking in a chronic but asymptomatic state called a biofilm.
Tricked-Out Immune Cells Could Attack Cancer
New cell-engineering technique may lead to precision immunotherapies.
Neural Networks Adapt to the Presence of a Toxic HIV Protein
HIV-associated neurocognitive disorders (HAND) afflict approximately half of HIV infected patients.
HIV Protein Manipulates Hundreds of Human Genes
Findings search for new or improved treatments for patients with AIDS.
Breaking the Brain’s Garbage Disposal
The children’s ataxia gene problem turned out to be not such a big deal genetically — it was such a slight mutation that it barely changed the way the cells made the protein.
Flesh-Eating Bacteria Work Together
Scientists recently discovered different strains of deadly flesh-eating bacteria working together to spread infection and they now have a better understanding of the role of the toxins they produce. The discovery could change how the illness and other diseases are treated.
Utilizing Antibodies from Ebola Survivors
A collaborative team from The University of Texas Medical Branch at Galveston, Vanderbilt University, The Scripps Research Institute and Integral Molecular Inc. have learned that antibodies in the blood of people who have survived a strain of the Ebola virus can kill various types of Ebola.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!