Corporate Banner
Satellite Banner
Immunology
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Sigma Life Science to Distribute Olink Bioscience's Protein-Protein Interaction Assay

Published: Tuesday, February 12, 2013
Last Updated: Tuesday, February 12, 2013
Bookmark and Share
The products complement Sigma's antibody, RNAi and gene editing technologies.

Sigma-Aldrich® Corporation announced that Sigma® Life Science have signed an agreement to distribute worldwide Olink Bioscience's Duolink® In Situ products, which present a new disruptive method for imaging and measuring protein-protein interactions in unmodified cells. The Duolink In Situ products work in tandem with Sigma Life Science's broad antibody portfolio and will allow researchers to target the precise protein of interest and enable robust analyses and imaging. 

The versatile technology, among other applications, can be used in combination with CompoZr® Zinc Finger Nuclease-mediated gene editing to track knocked-out or knocked-in genes and with MISSION® RNA interference technologies to track protein expression following gene knockdown. "The synergy between Olink and Sigma Life Science's global support network will help the scientific community to access and to accelerate the adoption of the powerful Duolink technology," said Josef Zihlmann, Vice President at Sigma Life Science.

The proprietary PLA® technology allows Olink's Duolink In Situ products to quickly image the locations of single protein-protein interactions regardless of strength, measure protein expression levels, and identify relative changes in post-translational modification events. The technique relies upon two target-specific primary antibodies, a "plus" and "minus" pair of species-specific PLA probes conjugated to DNA oligos that—when a target pair of proteins are in close proximity—can hybridize to trigger a rolling circle amplification reaction. Fluorescent detection probes bind the several hundred-fold amplified DNA at high density, allowing visualization of single protein—protein interactions inside an intact cell using a standard fluorescence microscope.

"The ability to visualize these protein-protein interactions in unmodified cells under endogenous expression and at very low levels is a fundamental advance that allows greater insight into basic biology, pathways, spatial phenomena and potential therapeutic targets in those pathways," said Zihlmann.

More than 350 publications in the past six years have relied upon the Duolink method, which is featured in an average of five publications per week by academic and pharmaceutical researchers. The Sigma Life Science Prestige® antibody collection complements the technology with its portfolio of more than 14,000 antibodies that have been validated by immunohistochemistry, a key requirement for Duolink In Situ products to target specific proteins of interest.

"We believe the agreement with Sigma Life Science will build awareness and develop the market for Duolink In Situ through their global sales and support organization serving our growing customer base locally," said Simon Fredriksson, CEO and President of Olink Bioscience. "Sigma Life Science's extensive product range for cell biology is a perfect context for customers interested in Duolink In Situ."


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Sigma-Aldrich Stockholders Approve Merck Acquisition
The transaction, expected to close mid-2015, remains subject to the satisfaction of the closing conditions set forth in the merger agreement, including regulatory approvals.
Monday, December 08, 2014
Scientific News
Iron Nanoparticles Make Immune Cells Attack Cancer
Researchers accidentally discover that nanoparticles invented for anemia treatment can trigger the immune system’s ability to destroy tumor cells.
Culex Mosquitoes Do Not Transmit Zika
A study of the Culex species mosquito appears to show that the species does not transmit Zika virus.
Bacteria Use Ranking Strategy to Fight Off Viruses
Researchers have explained why microbes store virus confrontation information sequentially, with most recent attacks first.
Molecular Switch Aids Immune Therapy
Researchers identify strategy to maximise effectiveness of immune therapy through molecular switch controlling immune suppression.
Reprogramming Lymph Nodes to Fight MS
Bioengineers work to reprogram lymph node function to fight multiple sclerosis.
Antibodies Block Norovirus’ Entrance into Cells
Scientists have uncovered a mechanism in the human body that targets and successfully blocks noroviruses.
Probe Detects Histone Modifications in Cells
Scientists have developed an antibody probe that can be used to monitor the dynamics of histone modification.
Gut Pathogens Thrive on Body's Tissue-Repair Mechanism
Researcher have discovered that harm caused by pathogens in the intestinal tract benefit from immune system response to damaged intestinal lining.
Eisai Establishes AiM Institute
The Andover innovative Medicines (AiM) Institute will develop innovative precision medicines for hard-to-treat conditions.
Antibodies that Target Holes in HIV's Defence Identified
Scientists suggest 'holes' in HIV sugar sheild can be targeted by antibodies.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!