Corporate Banner
Satellite Banner
Immunology
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Syngene's G:BOXChemi Used in Microbial Research at Wageningen UR

Published: Friday, June 07, 2013
Last Updated: Friday, June 07, 2013
Bookmark and Share
To contribute to understanding how bacterial immune systems function.

Syngene has announced that one of its G:BOXChemi imaging systems is being used at the Wageningen UR (University & Research centre) to visualize and analyze bacterial proteins as part of a research programme to understand the molecular mechanisms behind bacterial immune systems.

Scientists in the Laboratory of Microbiology at Wageningen UR are using a G:BOXChemi system to accurately image both chemiluminescent and fluorescently labelled Western blots of multi-functional protein complexes belonging to the CRISPR/Cas proteins.

These proteins degrade foreign DNA, thus protecting bacteria from infection and by studying them, the scientists hope to unravel how bacterial immune systems function.

Determining pathways of bacterial immunity could lead to a number of applications, such as being able to immunize industrially important bacteria used in food production and large-scale fermentations against phage infection.

Raymond Staals, a Researcher at Wageningen UR said: “We undertake RNAi studies and then assess protein expression on Western blots. However, performing chemi blots in our facility used to be such a hassle as we’d have to develop X-ray films and then take photos. We decided it needed to be simpler so tried using a laser-based scanner but this was limited to chemi blots and it was complicated to work with as we weren’t given any instruction on how to use it.”

Staals continued: “We want to work with fluorescent and chemi Westerns and need a system which can analyze a whole range of different dye types. We looked at two CCD-based imagers and decided on the G:BOXChemi because the system is very user-friendly as the GeneSys software guides you through everything. We currently use the G:BOXChemi regularly to image agarose and acrylamide DNA or RNA gels and sometimes to image plate-assays of bacteria expressing fluorescent luciferase. The system is very popular as many other researchers and students come to use the G:BOXChemi to image DNA gels now instead of using our old gel doc as the quality of the images is so much better.”

Laura Sullivan, Syngene’s Divisional Manager concluded: “We are pleased to discover the Laboratory of Microbiology at Wageningen UR has chosen Syngene’s system to be part of their specialist microbial genetics programme. Their work demonstrates that a G:BOX Chemi multi-functional image analyzer is ideally suited for use by students and experienced scientists alike and is versatile enough to be used with confidence to produce great results regardless of the blot or gel type used.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,100+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Getting a Better Look at How HIV Infects and Takes Over its Host Cells
A new approach, developed by a team of researchers led by The Rockefeller University and The Aaron Diamond AIDS Research Center (ADARC), offers an unprecedented view of how a virus infects and appropriates a host cell, step by step.
Untangling Disease-Related Protein Misfolding
Work advances understanding of genetic forms of thrombosis, emphysema, cirrhosis of the liver, neurodegenerative diseases and inflammation, among others.
Developing a More Precise Seasonal Flu Vaccine
During the 2014-15 flu season, the poor match between the virus used to make the world’s vaccine stocks and the circulating seasonal virus yielded a vaccine that was less than 20 percent effective.
Fighting Cancer with Borrowed Immunity
A new step in cancer immunotherapy: researchers from the Netherlands Cancer Institute and University of Oslo/Oslo University Hospital show that even if one's own immune cells cannot recognize and fight their tumors, someone else's immune cells might.
Loss Of Y Chromosome Increases Risk Of Alzheimer’s
Men with blood cells that do not carry the Y chromosome are at greater risk of being diagnosed with Alzheimer’s disease. This is in addition to an increased risk of death from other causes, including many cancers. These new findings by researchers at Uppsala University could lead to a simple test to identify those at risk of developing Alzheimer’s disease.
Immune Cells Remember Their First Meal
Scientists at the University of Bristol have identified the trigger for immune cells' inflammatory response – a discovery that may pave the way for new treatments for many human diseases.
"Sunscreen" Gene May Guard Against Melanoma
USC-led study reveals that melanoma patients with deficient or mutant copies of the gene are less protected from harmful ultraviolet rays.
Myeloid-Derived Suppressor Cells Play Role in Tumor Growth
Researchers at Baylor College of Medicine have reported a new mechanism that helps cancer cells engage myeloid-derived suppressor cells.
Drug Might Help Treat Sepsis
A DNA enzyme called Top1 plays a key role in turning on genes that cause inflammation in mouse and human cells in response to pathogens. A drug blocking this enzyme rescued mice from lethal inflammatory responses, suggesting a potential treatment for sepsis.
Large-scale HIV Vaccine Trial to Launch in South Africa
NIH-funded study will test safety, efficacy of vaccine regimen.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!