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Multiplexed Cell- and Bead-based Assays in a No-wash Format for Biologics Screening and Characterization
David Onley, Lucy Chammas, Diana Caracino and Paul G. Wylie

Here we present the results generated from multiplexed no-wash protocols using TTP Labtech’s mirrorball, a laser scanning cytometer.

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Development of a Homogenous RSV Neutralization Assay on the Mirrorball Fluorescence Cytometer: A Simple, Safe and Robust Approach for High-throughput Screening
Anne F. Hammerstein and Lucy Chammas

Here we present the development and implementation of a simple RSV-neutralization assay on TTP Labtech’s mirrorball fluorescence cytometer.

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A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
Mira Genser-Nir, Sharon Daniliuc, Marina Tevrovsky, Roni Hazan Brill, Yuliya-Yael Miropolski, David Fiorentini.

An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications.

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Platform Agnostic Data Processing Routine for Targeted and Untargeted Metabolite Identification in Drug Discovery
Richard Lee,1 Vitaly Lashin,2 Andrey Paramonov,2 Alexandr Sakharov,2 Alexey Aminov2

A discussion of a new informatics solution that addresses common challenges in metabolite identification and characterization--from LC/MS data processing and metabolites prediction to reporting and databasing of assembled biotransformation knowledge.

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Molecular Abnormalities in Schizophrenia: Neurotransmission, Oxidative Stress, and Apoptosis
Elizabeth J. Want,1 Hendrik Wesseling, 2 Elaine Holmes, 1 and Sabine Bahn 2

A multi-platform approach was employed to analyse distinct brain regions implicated in schizophrenia.

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Accurate Mass Spectral Database: Harnessing the Power of High Performance Mass Spectrometry at Long Last
Lorne Fell, Viatcheslav Artaev, Kevin McNitt, Steve Robles, Albert Lebedev

Standard mixtures comprising of alkanes, PAHs, semivolatiles, and pesticides were analyzed using a high resolution time-of-flight (HRTOF) mass spectrometer—Pegasus GC-HRT (LECO Corporation, Saint Joseph,MI)—at 10 spectra-per-second (m/z40–300) in high resolution mode (25,000 at FWHH). The resulting chromatographic peaks were automatically found, deconvoluted, and curated into an Accurate Mass Library (AML).

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Super-resolution single molecule localization microscopy of the exocytotic machinery underlying insulin secretion.
Deirdre M. Kavanagh, Alison Dun, Rory R. Duncan, and Colin Rickman.

Single molecule imaging of the tSNARE proteins involved in insulin secretion.

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Automated Sample Preparation of Whole Blood for Therapeutic Drug Monitoring and Diagnostics by LC-MS using a Commercial Autosampler
Christian Berchtold1, Irene Wegner1,Timm Hettich1, Reto Bolliger2, Guenter Boehm2, Goetz Schlotterbeck1

In this poster the parameters necessary to automatically prepare whole blood samples for online LC-MS applications in the field of diagnostics and TDM have been investigated. A strategy and the most important parameters are shown for the optimization of a PAL RTC autosampler
for the preparation of whole blood samples.

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Development and Application of Quantitative Immunoassays for Major Milk Allergens Bos d 5 (β-lactoglobulin) and Bos d 11 (β-casein)
1Ross A. R. Yarham, 1Anna Kuklinska-Pijanka MSc, 1David Gillick, 1Elizabeth Young, 2Karine Adel Patient PhD, 2Hervé Bernard PhD, 1Martin D. Chapman PhD, 1James P. Hindley PhD

In this study, we sought to develop accurate, sensitive and reliable assays that would enable quantification of multiple milk allergens.

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Diagnosing Coral disease with Metabolomic Markers
Michael Ochsenkuehn

Application of untargeted metabolomics for the identification of disease markers in corals

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Scientific News
Flu Vaccine May Reduce Death Risk of Type 2 Diabetes
New research suggests that a new flu vaccine may reduce probability of type 2 diabetes patients being hospitalised with stroke and heart failure.
Gut Bacteria Older than Human Species
Some bacteria have lived in the human gut since before we were human, suggesting evolution could have a larger role inhuman bacterial makeup.
Evidence of Mosquito Transmitting Zika
A direct link between the Yellow fever mosquito and Zika transmission has been found following investigation into selective mosquito control.
Antibody-Based Drug for Multiple Sclerosis
New antibody-based drug paves the way for new strategies for controlling and treating multiple sclerosis.
Three-Drug Combinations Counter Antibiotic Resistance
Research shows that combinations of three different antibiotics can treat resistant bacteria, even if they are ineffective independently.
Mapping Zika’s Routes to Developing Fetus
UC researchers show how Zika virus travels from a pregnant woman to her fetus, and also identified a drug that could stop it.
Treating HIV with Cancer-Fighting Gene Shows Promise
A type of gene immunotherapy that has shown promising results against cancer could also be used against HIV.
Protein Teams Activate T-Cells
Caltech researchers have discovered T-cell genetic switching is controlled by four proteins acting in a multi-tiered fashion.
'Antigen-Presenting Cell' Defends Against Cancer
Through advanced imaging, researchers have identified cells that encourages increases in immune system cancer defences.
Zika Epidemic Likely to End Within Three Years
A team of scientists has predicted that the current Zika epidemic is likely to end within three years because there will be too few people left to infect.
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