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Modelling CLL cell and T-cell Migration in a Dynamic Circulating Model of CLL
Elisabeth Walsbyl, Paul Brennan', Guy Pratte, Andrea Buggins3, Tanja N Hartmann'', Chris Fegan1 and Chris Pepper'

We have recently developed a novel circulating model of chronic lymphocytic leukaemia (CLL) that mimics the transient interactions that take place between circulating lymphocytes and vascular endothelium. Here we show that both normal and malignant lymphocytes actively underwent transendothelial migration.

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Quantification of Natural Killer Cell-Mediated Cytotixicity using Celigo Imaging Cytometry
Leo L. Chan, Srinivas S. Somanchi, Kelsey Rosbach, Dean A. Lee

Time-course tracking of % lysis eliminates multiple controls & the effect of non-uniform cell seeding in the final cytotoxicity calculation. The # of cells used is less than the cells needed for Release assays & Flow Cytometry. Flow cytometry & Release assays require a seeding density of 100K target cells increasing the number of effector cells to the millions. The visual observation of ADCC or CDC on the images can be convincing to conclude the functionality of antibodies or complements.

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Non-invasive Liquid Biopsy
Frauke Henjes1, Lise Aagaard1, Darren Ellis1, Hannah Lindstroem1, Morten Oksvold2,3, Axl Neurauter1, Ketil W. Pedersen1.

Paramagnetic Dynabeads® magnetic beads provide a versatile, automation friendly tool for fast sensitive and high throughput isolation that can be tailored to isolate specific circulating biomarkers such as cells, exosomes and nucleic acids.

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Novel culture medium using a small-molecule agonist of thrombopoetin receptor
Hondo M1; Nishino T2; Inamura M1

ReproHSC medium for the culture of hematopoietic stem cells. facilitates the culture and expansion of CD34+/CD38- cells that retain their HSC properties

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Flexible automated platform for blood group genotyping on DNA microarrays
S. Paris1, D. Rigal1, V. Barlet1, M. Verdier1, N. Coudurier2, P. Bailly3, J.-C. Brès1,4

The purpose of this project was to set up and validate a flexible robotic platform using 96-well DNA microarray for multiplex blood group genotyping.

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THE MASTOCYTOSIS SOCIETY SURVEY ON MAST CELL DISORDERS: Part 2-Clinical Experiences, Co-Morbidities, Diet, Families and Opinions
aNancyRussell, DrPH, aSusanJennings, PhD, aBlairJennings, BS, aValerie Slee , RN, BSN, aLisa Sterling, BS, bMariana Castells, MD, PhD, FAAAAI,cPeter Valent, MD, bCem Akin, MD, PhD, FAAAAI

Mast cell diseases such as mastocytosis and mast cell activation syndromes involve abnormal proliferation or activation of these cells leading to many potentially debilitating symptoms. In order to determine the characteristics and experiences of people known or suspected to have a mast cell disorder, The Mastocytosis Society (TMS), a U.S. based patient advocacy, research and education organization, conducted a survey of patients.

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Safety Of A Sublingual Tablet of House Dust Mite Allergen Extracts In An Environmental Exposure Chamber Study
Michel Roux, Agnès Viatte, Robert K. Zeldin

In an environmental exposure chamber (EEC) study, treatment with HDM sublingual tablets in patients with HDM-associated allergic rhinitis was generally well tolerated regardless of dose. While asthma and related symptoms were more common during the peri-EEC challenge periods, rates did not differ between active and placebo treatment.

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Safety Of The 300 IR and 500 IR Doses Of A House Dust Mite Allergen Extracts Sublingual Tablet In Adults with Allergic Rhinitis
Hélène Nguyen, Michel Roux, Josiane Cognet-Sicé, Robert K. Zeldin

In a randomized, DBPC study in patients with HDM-associated allergic rhinitis, treatment with HDM sublingual tablets at doses of 300IR and 500IR was associated with a favorable safety profile. There was no appreciable difference in tolerability between the tested doses.

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Response to ragweed allergen provocation in the Red Maple Trials Allergen Challenge TheatreTM
Suzanne Kelly, PHD;Jacob Karsh, MDCM, FRCPC; Jimmy Yang, MBA; William H. Yang, MD, FRCPC, FAAAAI

Response to ragweed allergen provocation in the Red Maple Trials Allergen Challenge Theatre

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Field Performance of a New Technology with the Potential to Identify Allergy and Asthma Triggers
Julian Gordon, PhD, Prasanthi Gandhi, MBA, MPH1, Jack A.Gilbert, Jarrad T. Hampton-Marcell

This work introduces a very simple plug-and-play device that can sample air at a high volume flow rate with no moving parts. Aerosol particles are captured on electrodes and eluted for testing. We show that performance of the device is substantially equivalent to capture by pumping through a filter for immunological and molecular analysis.

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Scientific News
Freshly Squeezed Vaccines
Microfluidic cell-squeezing device opens new possibilities for cell-based vaccines.
New Target for Treating Rheumatoid Arthritis
Many affected individuals still suffer with progressive joint damage, despite treatment. This study has discovered a new treatment target which could open a door to preventing this ongoing damage.
Shining A New Light On The Immune System
Scientists at the University of St Andrews have developed a revolutionary method of identifying cells of the immune system with “molecular fingerprints” which could pave the way for the rapid detection of conditions such as leukaemia and lymphoma from a small blood sample.
Promising Malaria and Dengue Vaccines Will Not Defeat Diseases
Although vaccination is a key part of the control of disease GlobalData stress the importance of a multi-dimensional approach.
'Redesigned' Antibodies May Neutralize HIV
With the help of a new computer program researchers have made structural alterations to an antibody, increasing its ability to tackle the AIDS virus.
How Early Childhood Vaccination Reduces Leukemia Risk
Chronic infections push ‘pre-leukemia’ cells, common in newborns, into malignancy.
Novel Class Of Antimicrobials Kills Multidrug Resistant-Tuberculosis
Researchers hope that they can end the global TB crisis by utilizing a novel bactericidal mechasnism.
Inflammation Stops The Clock
Researchers at Trinity College Dublin and the University of Pennsylvania have uncovered an important link between our body clock and the immune system that will have relevance to the treatment of inflammatory and infectious diseases.
Designing Better Medical Implants
A team of MIT researchers have discovered a novel method for reducing the typical immune system rejection response when implanting biomedical devices into the body.
Finding Should Enhance Treatments that Stop Immune System Attacks
Research published in the journal Immunity describes how regulatory T cells can cure inflammatory disease.
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