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Tuesday, September 02, 2014
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PRESEPSIN, A SOLUBLE CD14-SUBTYPE, A POSSIBLE NEW BIOMARKER INCREASES IN SEPTIC PATIENTS’ PLASMA FROM PEDIATRIC DEPARTMENT.
Hayato YAMAGUCHI1), Satoshi KIMURA1), Seiji FUKUOKA1), Emiko NAKAMA1), Hideyasu OTO2), Makoto INOUE2), Takashi SOGA2), Shigetaka KITAZAWA2), Yoh UMEDA2)

Increased plasma concentration of soluble CD14-subtype (presepsin) was observed in pediatric patients with bacteremia. Presepsin could be a possible biomarker of sepsis in pediatric patients, however, their reference interval in children could be lower than that of adults. More studies with larger number of samples are required to confirm the result.

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A Rapid and Effective Tool for Monitoring Monoclonal Antibody Production
B. Bhullar, Ph.D., Carlina Hui, B.Sc., M. Hariharan, M.Sc., J. Hewitt, B.Sc , and N. Vats, Ph.D.

Monoclonal antibodies (Mab) are used in a variety of fields from diagnostics to therapeutics. Manufacturers of in vitro diagnostics (IVD) commonly use third-party suppliers to meet their needs for specified monoclonal antibodies.

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New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples
Binggang Sun, Jingjing Yao, Jiaming Hu and Shaoquan Ji

We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.

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Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies
Hong Zhang, Kelly Lundsten, Kevin Williams, Beibei Ding, Xiao Lin, Xifeng Wang, Jie Zhou, Dzung Nguyen, and John Ransom

Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.

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Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer
Divekar AA, Lee MJ, Kakuta M, Nitta N, Furuki M, Yang XF and Ransom JT

SP6800, a novel spectral analyzer from Sony Corporation, uses an innovative optics system that collects all emitted light and eliminates the need for band pass filters. Our data show that the SP6800 can perform all the analysis similar to a traditional cytometer with the added feature of separating spectrally overlapping fluorophores.

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Comparison of Three Methods for the Evaluation of Cytokine Storm Risk in Early and Clinical Stage Biopharmaceutical Development
Gary dos Santos and Emer Clarke

Objective: To identify an assay that can accurately predict the risk of CRS and CS associated with investigational biotherapeutics. A comparison of three methods were used: (a) immobilization of test antibody on plastic, (b) co-culture of PBMC's on HUVEC's and (c) pre-culture of PBMC's at high cell density.

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Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies
W. John Martin

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.

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Hypothesis of an Existence of a Reverse Pathway (Rp) which Passes Genetic Information from Polypeptide Antigens to Ig Genes in B-Lymphocytes
Victor J. Alexander

Results of this research will bring new more effective treatment methods of many immune related diseases, including cancer.

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A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope

Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.

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Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation
Wu-Bo Li and Michael Goldblatt

Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.

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Showing Results 1 - 10 of 21
Scientific News
Understanding and Improving the Body's Fight Against Pathogens
A*STAR scientists find new targets for modulating antibody response.
NIH to Launch Human Safety Study of Ebola Vaccine Candidate
Trial is First in Series of Accelerated Safety Studies of Ebola Vaccines.
Gut Bacteria that Protect Against Food Allergies Identified
Common gut bacteria prevent sensitization to allergens in a mouse model for peanut allergy, paving the way for probiotic therapies to treat food allergies.
MU Researchers Discover Protein's Ability To Inhibit HIV Release
TIM-family proteins have the ability to block the release of HIV and other viruses.
Bowel Disease Gene Discovery
Genetic changes that occur in patients with the bowel condition Crohn’s disease could hold clues to fighting the illness.
Test Reliably Detects Inherited Immune Deficiency in Newborns
NIH-supported study suggests that early diagnosis of severe combined immunodeficiency leads to high survival rates.
500 Million Year Reset for the Immune System
A single factor can reset the immune system of mice to a state likely similar to what it was 500 million years ago, when the first vertebrates emerged.
Sequencing Identifies Gene Variant Responsible for Lupus
Research demonstrates it is feasible to identify the individual causes of lupus in patients by using DNA sequencing, allowing doctors to target specific treatments to individual patients.
Test Reliably Detects Inherited Immune Deficiency in Newborns
NIH-supported study suggests that early diagnosis of severe combined immunodeficiency leads to high survival rates.
Immune Cells get Cancer-Fighting Boost From Nanomaterials
Yale researchers used bundled carbon nanotubes to incubate cytotoxic T cells.
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