|Early Immunogenicity Risk Mitigation|
Chloé Ackaert, Brecht Hoedemaekers, Sofie Pattijn and Philippe Stas
The ability of a drug candidate to induce an unwanted immune response can be a significant hurdle during drug development and a reason for product failures. Unwanted immunogenicity can cause loss of efficacy, altered pharmacodynamics and stability problems. .
|MAB Discovery Technology: A Smart Way to Highly Diverse and Functional Therapeutic Antibodies|
MAB Discovery GmbH developped a highly integrated process which provides diverse antibodies by starting with a high number of B cells and filtering the relevant antibodies by an early-on functional screening.
|PRESEPSIN, A SOLUBLE CD14-SUBTYPE, A POSSIBLE NEW BIOMARKER INCREASES IN SEPTIC PATIENTS’ PLASMA FROM PEDIATRIC DEPARTMENT.|
Hayato YAMAGUCHI1), Satoshi KIMURA1), Seiji FUKUOKA1), Emiko NAKAMA1), Hideyasu OTO2), Makoto INOUE2), Takashi SOGA2), Shigetaka KITAZAWA2), Yoh UMEDA2)
Increased plasma concentration of soluble CD14-subtype (presepsin) was observed in pediatric patients with bacteremia. Presepsin could be a possible biomarker of sepsis in pediatric patients, however, their reference interval in children could be lower than that of adults. More studies with larger number of samples are required to confirm the result.
|A Rapid and Effective Tool for Monitoring Monoclonal Antibody Production|
B. Bhullar, Ph.D., Carlina Hui, B.Sc., M. Hariharan, M.Sc., J. Hewitt, B.Sc , and N. Vats, Ph.D.
Monoclonal antibodies (Mab) are used in a variety of fields from diagnostics to therapeutics. Manufacturers of in vitro diagnostics (IVD) commonly use third-party suppliers to meet their needs for specified monoclonal antibodies.
|Exploring the Therapeutic Potential of a Peptide Derived from a Poxviral Immune Evasion Protein|
•9R-VIPER is an effective at TLR4 and TLR2 signal inhibition than VIPER.
•The structure of 9R-VIPER and the mutant peptide L6AE10A were investigated by NMR.
•Important structural information was uncovered and an explanation was found for the loss
of activity when important residues are changed.
|New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples|
Binggang Sun, Jingjing Yao, Jiaming Hu and Shaoquan Ji
We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.
|Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies|
Hong Zhang, Kelly Lundsten, Kevin Williams, Beibei Ding, Xiao Lin, Xifeng Wang, Jie Zhou, Dzung Nguyen, and John Ransom
Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.
|Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer|
Divekar AA, Lee MJ, Kakuta M, Nitta N, Furuki M, Yang XF and Ransom JT
SP6800, a novel spectral analyzer from Sony Corporation, uses an innovative optics system that collects all emitted light and eliminates the need for band pass filters. Our data show that the SP6800 can perform all the analysis similar to a traditional cytometer with the added feature of separating spectrally overlapping fluorophores.
|Comparison of Three Methods for the Evaluation of Cytokine Storm Risk in Early and Clinical Stage Biopharmaceutical Development|
Gary dos Santos and Emer Clarke
Objective: To identify an assay that can accurately predict the risk of CRS and CS associated with investigational biotherapeutics. A comparison of three methods were used: (a) immobilization of test antibody on plastic, (b) co-culture of PBMC's on HUVEC's and (c) pre-culture of PBMC's at high cell density.
|<< || 1 2 3 4 || >>|
|Showing Results 11 - 20 of 34||