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Tuesday, July 22, 2014
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Improving Cell-Mediated Cytotoxicity Assessment through the Use of an Automated Luminescent ADCC Assay
Brad Larson, Sumant Dhawan, Shalini Wadwani, and Peter Banks

Assays that can assess the ability of a biosimilar to act in a manner similar to the original biologic have seen increased interest. This poster describes the use of a non-radioactive luminescent chemistry to simplify the assay process and provide improved data quality.

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Modular Bench Top Automation
Paul Held, Dean Mulyk, Lenore Buehrer and Grace Mangialardi

ELISA is one the most utilized assay formats in biomedical research. Numerous clinical, veterinary, and research assays use the specificity of antibodies to identify a diverse array of analytes from any number of different matrices. This poster details a modular bench top workstation capable of automating the assay process steps of most conventional ELISA assays.

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Optical Microchip Sensors for Multiplexed Detection of Biological Pathogens
D. Bhatta, A. Michel, M. Marti Villalba, G. D. Emmerson, I. J. G Sparrow, M. B. McDonnell, E. A. Perkins , R. W. Ely and G. A. Cartwright

SpectroSens, a multi-channel optical microchip sensor system suitable for rapid, label-free multiplexed detection of a wide range of bio-hazardous agents is presented. Optical chips containing multiple high-precision planar Bragg gratings are exploited as low-cost, robust refractive index sensors.

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A Mix-and-Read Cell-Based Assay for Antibody Screening Against Epidermal Growth Factor Receptor
Wayne Bowen, David Onley, Tristan Cope

The conventional antibody screening assay based on antibody-antigen binding has been enzyme-linked immunosorbent assay (ELISA). While tedious and consuming, ELISA has proved sufficient for the identification of antibodies directed against secreted antigens. However, cell surface antigens (e.g. GPCRs) provide challenges for ELISA due to the shortage of soluble antigens and high variability resulting from loss of cells during wash procedures.

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Gene expression analysis of CD14+ monocytes immunomagnetically separated directly from whole blood: adaptation of protocols towards clinical trial requirements
Gregor Winkels1, Ines Dischinger1, Katharina Bublitz1, Evert Luesink2, Nanguneri R. Nirmala2, Frank Staedtler2, Keith J. Johnson2, Alena Fitz1, Sabrina Schmitz1, Dirk Dietrich1, Sonja Balzer1, Sabine Classen1, Silvia Rüberg1, Uwe Janssen1, and Bernhard Gerstmayer1

Peripheral blood is widely used as starting material for biomarker discovery and validation using molecular biology technologies. The vast majority of currently published transcriptome data is based on RNA derived either from stabilized whole blood or peripheral blood mononuclear cells (PBMCs). Here, gene expression profiling studies and SOPs for fast, easy and specific manual or automated isolation of monocytes directly from whole blood are being described.

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In silico screening of new potential TCR/CollagenII-MHCII inhibitors against rheumatoid arthritis
Davide Pirolli, Francesco Ria, Bruno Giardina and Maria Cristina De Rosa

Residues 261-273 of type II collagen bound to the MHC class II allele HLA-DR4 play a crucial role in rheumatoid arthritis. The protein–protein interactions between TCR and CII-MHCII complex may therefore serve as targets for the development of new drugs against RA. The aim of this study is to develop a pharmacophore virtual screening followed by molecular docking and dynamics calculations leading to the identification of new TCR/CII-MHCII inhibitors.

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New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells
Victor Alexander, PhD.; Anthony Passerini, PhD; Emir Hodzic, PhD.

We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue

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Investigating Viral dynamics
Sukhdeep Singh

Analysis of SIV next generation sequencing data for the estimation of escape rates in different animal groups and tissue compartments.

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Hepatitis B virus (HBV) and Human immunodeficiency virus (HIV) antibodies detected by peptide microarrays
ahmed Abd El Wahed1, Ulrike Beutling2, Ronald Frank2, Gerhard Hunsmann1, and Hans-Joachim Fritz3

HBV and HIVenv chips with overlapping oligopeptides encompassing the full amino acid sequences of HBV and HIV polypeptides were produced. In addition, a chip displaying a library of random 4608 different 15-mers peptides (4608-RPL) was prepared. Both chips were used for analyzing monoclonal antibodies and sera from HIV- and HBV-infected individuals. 4608-RPL could be used for identifying target sequences of antibodies without prior knowledge of the corresponding immunizing antigen.

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Showing Results 11 - 19 of 19
Scientific News
NIH Scientists Identify Gene Linked to Fatal Inflammatory Disease in Children
Repurposed drugs may offer first potential therapy.
Injected Vaccine Could Help Eradicate Polio
Re-introducing a type of polio vaccine that fell out of favour in the 1960s could hasten eradication of the disease.
New Possibilities for Leukaemia Therapy with a Novel Mode of Cancer Cell Recognition
A new class of lipids in human leukaemia cells trigger an immune response to kill the cells.
Cellular Force That Drives Allergy and Asthma Can be Blocked by Interferon
Type I interferons block the development of allergy- and asthma-driving Th2 cells.
A Key Step Toward a Safer Strep Vaccine
Gene discovery identifies molecular pathway to potential preventive treatment.
Gum Disease Bacteria Selectively Disarm Immune System, Penn Study Finds
New study shows that bacteria responsible for many cases of periodontitis cause dysbiosis.
Immune Response Affects Sleep and Memory
Sickness-induced insomnia is common because of link between brain and immune system.
Newborns Exposed to Allergens May Have Lower Allergy and Asthma Risk
Newborns exposed to household germs, pet and rodent dander and roach allergens during their first year of life appear to have lower risk of developing asthma and allergies.
Ubiquitous Protein Controls Copying of Resistant DNA
Researchers have demonstrated how the protein could put antibiotic-resistant bugs in handcuffs.
Humanity's Overall Environmental Footprint is Not Sustainable
Substantial, fundamental changes in the world economy are required to reduce humanity's overall environmental footprint to a sustainable level.
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