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Determining the Appropriate Sample Load for Western Blots
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Bio-Rad Laboratories

Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak signals. 

This application note describes an assay development experiment to determine the appropriate protein load for target and control detection prior to performing the actual western blot experiment.

Further Information

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