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Experimental MERS Vaccine Shows Promise in Animal Studies
A two-step regimen of experimental vaccines against Middle East respiratory syndrome (MERS) prompted immune responses in mice and rhesus macaques, report National Institutes of Health scientists who designed the vaccines.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
New Weapon in the Fight Against Blood Cancer
This strategy, which uses patients’ own immune cells, genetically engineered to target tumors, has shown significant success against multiple myeloma, a cancer of the plasma cells that is largely incurable.
Scientists Create CRISPR/Cas9 Knock-In Mutations in Human T Cells
In a project spearheaded by investigators at UC San Francisco, scientists have devised a new strategy to precisely modify human T cells using the genome-editing system known as CRISPR/Cas9.
Researchers Develop Vaccine that Protects Primates Against Ebola
A collaborative team from The University of Texas Medical Branch at Galveston and the National Institutes of Health have developed an inhalable vaccine that protects primates against Ebola.
Universal Flu Vaccine in the Works
A new study has demonstrated a potential strategy for developing a flu vaccine with potent, broad protection.
Immunotherapy Shows Promise for Myeloma
A strategy, which uses patients’ own immune cells, genetically engineered to target tumors, has shown significant success against multiple myeloma, a cancer of the plasma cells that is largely incurable.
Immune System 'On Switch' Breakthrough Could Lead to Targeted Drugs
A crucial 'on switch' that boosts the body's defenses against infections has been successfully identified in new scientific research.
HIV Control Through Treatment Durably Prevents Heterosexual Transmission of Virus
NIH-funded trial proves suppressive antiretroviral therapy for HIV-infected people effective in protecting uninfected partners.
Adaptimmune's Novel Cancer Therapeutics Show Positive Clinical Trial Results
The company has announced that positive data from its Phase I/II study of its affinity enhanced T-cell receptor (TCR) therapeutic targeting the NY-ESO-1 cancer antigen in patients with multiple myeloma has been published.
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Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
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Tecan Group Ltd.

Bioassays designed to measure antibody-dependent cell-mediated cytotoxicity (ADCC) are particularly useful when looking to understand and characterize mechanism of action, and to quantify biologic potency and stability of target compounds during discovery and development.

However, the labor and skill required to isolate primary natural killer cells for ADCC or to maintain alternative cell lines in culture with any level of reproducibility remains problematic, with high costs in consumable reagents and in purchasing and maintaining basic equipment.

Promega has developed reporter bioassays that use activation of gene transcription through NFAT (nuclear factor of activated T-cells) as an earlier measure of ADCC. The bioassays use frozen, thaw-and-use effector and target cells and eliminates the need for cell culture maintenance. The use of frozen cells also decreases assay variability. 

The ADCC Reporter Bioassay requires six-hour incubation with cells and antibody for optimal NFAT activation and luciferase expression to occur.The Tecan Infinite® 200 PRO, equipped with an external module that enables simultaneous adjustment of CO2 and O2, provides an alternative method enabling scientists without access to a cell culture facility to easily perform the bioluminescent reporter bioassay.

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