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  Events - October 2014


2 days Hands-on qPCR (Czech language), 2 denní praktický kurz qPCR

20 Oct 2014 - 21 Oct 2014 - Prague, Czech Republic



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2 days Hands-on qPCR (Czech language), 2 denní praktický kurz qPCR Určené pro: Začátečníci a středně pokročilí uživatelé qPCR Doporučené znalosti: Základy molekulární biologie Popis: Základní qPCR kurz s praktickýma cvičeníma. Po absolovování získáte komplexní přehled o možnostech qPCR, jak ho používat a analyzovat výsledky. Den 1 Program kurzu: Základní qPCR teorie Aplikace a možnosti qPCR. Porovnání qPCR s konvenčním PCR Přehled současně dostupných technologií (SYBR green I, hydrolyzační próby, molecular beacons, atd.) Přehled qPCR instrumentů a jejich využití Navrhování primerů Problém s vytvářením primerů-dimerů a jak ho minimalizovat Navrhování prób Experimentální design a optimalizace Základní zpracování dat a jejich analýza Den 2 Program kurzu: Úvod do kvantifikačních principů Kvantifikační strategie, jejich použití a limity Výpočty pomocí různých relativních kvantifikačních metod Normalizační strategie qPCR dat In situ kalibrace pro odhalení inhibice ve vzorcích Absolutní kvantifikace

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