Corporate Banner
Satellite Banner
Informatics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Bio-Rad’s New Image Lab™ Software Automates Total Protein Normalization for More Accurate Western Blotting Analysis

Published: Sunday, October 14, 2012
Last Updated: Sunday, October 14, 2012
Bookmark and Share
Software Provides Alternative to Potentially Misleading Housekeeping Proteins

Bio-Rad Laboratories, Inc. has introduced the new Image Lab 4.1 software for use with the ChemiDoc™ MP imaging system. The new software automates multiplex analysis, providing a faster, more accurate alternative for quantifying protein expression levels via western blotting.

Proving that quantitative differences of a target protein are a result of biological differences and not experimental variability (such as unequal protein loading) is critical when conducting western blotting experiments. Typically, scientists comparing gene expression levels use a housekeeping protein (HKP) as a loading control with the assumption that HKP expression does not change based on treatment type or sample origin. However, both of these conditions can alter HKP expression, undermining its value as a normalization control.

Total protein normalization has been shown to be more stable than HKP normalization regardless of conditions or sample type (ref DOI 10.1002/pmic.200400941). Also total protein normalization saves time by eliminating the need for multiple probing steps or optimizing a multiplex blot, both of which are often required to normalize with housekeeping proteins.

Normalizing total proteins via stain-free detection, a capability provided only by Bio-Rad‘s ChemiDoc MP imaging system, offers advantages including speed, increased linearity, and wider dynamic range, over other total protein detection methods such as SYPRO Ruby and Ponceau S.

Image Lab 4.1 automates total protein normalization of multiplex fluorescent western blots by extracting image data directly from the ChemiDoc MP imager. Traditional image analysis methods require researchers to extract data into a spreadsheet program and manually manipulate data to produce a result. With Image Lab 4.1 software, however, researchers can deliver normalized intensities automatically by selecting detection and normalization channels, then clicking the Normalization button. Normalization is available with chemiluminescent blots as well as multiplex fluorescent blots.

Stain-free total protein normalization is part of Bio-Rad’s new V3 Western Workflow, which speeds the western blotting process and provides researchers with the reassurance that their immunoblotting process is working as expected. Using stain-free detection, scientists can visualize protein separation without staining, verify protein transfer to the membrane, and validate the results by total protein normalization.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Royal Canadian Mounted Police to Use Bio-Rad’s KnowItAll® Spectroscopy Software for Analysis of Automobile Crime Scene Samples
Using the KnowItAll software to search multiple automobile paint layers found at a crime scene against the PDQ database, forensic scientists can determine the make, model, year, and manufacturing origin of the samples.
Sunday, March 30, 2014
Scientific News
Protein Nanocages Could Improve Drug Design and Delivery
HHMI scientists have designed and built 10 large protein icosahedra that are similar to viral capsids that carry viral DNA.
Connectome Map More Than Doubles Human Cortex’s Known Regions
Researchers at NIH have developed software that automatically detects the “fingerprint” of each of these areas in an individual’s brain scans.
Virus Inspired Cell Cargo Ships
Virus-inspired container design may lead to cell cargo ships following construction of ten large, two-component, icosahedral protein complexes.
Three-Drug Combinations Counter Antibiotic Resistance
Research shows that combinations of three different antibiotics can treat resistant bacteria, even if they are ineffective independently.
Supercomputing and Drug Discovery
New biotech company uses supercomputer simulations to speed up drug discovery and biotech molecule development.
Identifying Cancer Drug Targets Using 3D-Modelling
Researchers are now able to model genetic mutations manipulation of proteins that can potentially drive cancer.
Human Evolution Driven by Viruses
Study finds surprising percentage of protein adaptions in humans have been driven by viruses.
Earliest Sign of Alzheimer's Development Discovered
Researchers have identified the first physiological signs of late-onset Alzheimer's disease, underlining the importance of computational power in neurology.
Computational Method Offers Significant Boost in Finding New Cancer Targets
MIT and ARIAD Pharmaceuticals team have identified evidence of significant cancer mutations.
Drug Response Predicted by Cancer Cell Lines
Large-scale study could increase success rate of developing personalised cancer treatments.
Scroll Up
Scroll Down
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!