Corporate Banner
Satellite Banner
Informatics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Statistical Characterization of MRM-MS Assays for Quantitative Proteomics

Published: Friday, November 30, 2012
Last Updated: Friday, November 30, 2012
Bookmark and Share
MRM-MS assays are increasingly being deployed to measure and quantify peptides and proteins, this article provides a complete toolkit for the analysis and interpretation of MRM-MS experiments.

Abstract
Multiple reaction monitoring mass spectrometry (MRM-MS) with stable isotope dilution (SID) is increasingly becoming a widely accepted assay for the quantification of proteins and peptides. These assays have shown great promise in relatively high throughput verification of candidate biomarkers. While the use of MRM-MS assays is well established in the small molecule realm, their introduction and use in proteomics is relatively recent. As such, statistical and computational methods for the analysis of MRM-MS data from proteins and peptides are still being developed. Based on our extensive experience with analyzing a wide range of SID-MRM-MS data, we set forth a methodology for analysis that encompasses significant aspects ranging from data quality assessment, assay characterization including calibration curves, limits of detection (LOD) and quantification (LOQ), and measurement of intra- and inter-laboratory precision. We draw upon publicly available seminal datasets to illustrate our methods and algorithms.

The article is published online in the journal BMC Bioinformatics and is free to access.  



Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Proteomics for Systems Toxicology
MS-based proteomics is maturing into a robust technology for the measurement of proteome-wide exposure effects.
Monday, November 24, 2014
Identification of Psoriatic Arthritis Mediators in Synovial Fluid
This study represents the most comprehensive proteomic analysis of psoriatic arthritis synovial fluid, to date.
Friday, August 08, 2014
Potentials and Pitfalls of Clinical Peptidomics and Metabolomics
The major challenges in the search for "meta-markers", panels, or models thereof, are sensitivity, specificity and the avoidance of bias.
Monday, July 08, 2013
A Molecular-Modeling Toolbox Aimed at Bridging the Gap Between Medicinal Chemistry and Computational Sciences
Researchers from the University of Basel have designed a versatile single-click tools to assist medicinal chemists in performing two routine modeling tasks.
Monday, April 08, 2013
Identification of Plasma Lipid Biomarkers for Prostate Cancer by Lipidomics and Bioinformatics
This study explores a new approach to seeking biomarkers for prostate cancer and other diseases.
Monday, April 08, 2013
Optimal Precursor Ion Selection for LC-MALDI MS/MS
Researchers present two algorithms for inclusion list creation that formulate precursor ion selection as an optimization problem.
Tuesday, February 26, 2013
A Survey of Error-Correction Methods for Next Generation Sequencing
This article provides a review of error-correction algorithms for NGS data and their comparative evaluation.
Wednesday, February 20, 2013
An Adaptive Alignment Algorithm for Quality-Controlled Label-Free LC-MS
Researchers from Lund University have shown that a combination of an adaptive algorithm and rigorous quality control leads to more reliable and reproducible data analysis by extensive comparison to common label---free analysis approaches.
Wednesday, January 30, 2013
FAF-Drugs2: FREE ADME/TOX FILTERING TOOL to Assist Drug Discovery and Chemical Biology Projects
Researchers have developed FAF-Drugs2 to facilitate compound collection preparation, prior to (or after) experimental screening or virtual screening computations.
Thursday, September 27, 2012
MetiTree: A Web Application to Organize and Process Metabolomics Data
MetiTree combines processing, sharing, visualizing, and querying MSn data to help researches to identify metabolites of interest and decrease the time consuming task of identifying metabolites.
Friday, September 07, 2012
Identifying Mechanism-of-Action Targets for Drugs and Probes
A chemical information approach to drug-target association can guide therapeutic development and reveal applications to probe biology.
Friday, July 06, 2012
Interaction of Virstatin with Human Serum Albumin
Understanding the interaction of HSA with virstatin will be essential for the future designing of virstatin-inspired drugs.
Monday, June 11, 2012
Interfacing Medicinal Chemistry with Structural Bioinformatics
The observed aaRS-specific stability differences between key riboswitch structural elements could potentially be targeted to effect ligand-specificity in future drug discovery efforts.
Friday, May 11, 2012
Disease Model Curation Improvements at Mouse Genome Informatics
This paper evaluates the Comparative Toxicogenomics Database (CTD) and discusses its strengths and weaknesses.
Tuesday, April 10, 2012
Bioinformatics Tools for Mass Spectroscopy-Based Metabolomic Data Processing and Analysis
This paper provides an overview of the data processing tools available, offering a review of the collection of recent reports on the topic.
Tuesday, April 03, 2012
Scientific News
The Mystery of the Instant Noodle Chromosomes
Researchers from the Lomonosov Moscow State University evaluated the benefits of placing the DNA on the principle of spaghetti.
Web App Helps Researchers Explore Cancer Genetics
Brown University computer scientists have developed a new interactive tool to help researchers and clinicians explore the genetic underpinnings of cancer.
An Innovative Algorithm to Decipher How Drugs Work Inside the Body
Researchers at Columbia University Medical Center (CUMC) have developed a computer algorithm that is helping scientists see how drugs produce pharmacological effects inside the body.
How do Networks Shape the Spread of Disease and Gossip?
A team of mathematicians from Oxford University, University of North Carolina at Chapel Hill, and Rutgers University used a set of mathematical rules to encode how a contagion spreads, and then studied the outcomes of these rules.
AncestryDNA and Calico to Research the Genetics of Human Lifespan
Collaboration will analyze family history and genetics to facilitate development of cutting-edge therapeutics.
Informatics Tool Helps Scientists Prioritize Protein Modification Research
Researchers have developed a new informatics technology that analyzes existing data repositories of protein modifications and 3D protein structures to help scientists identify and target research on "hotspots" most likely to be important for biological function.
Software Differences can Skew Medical Scan Results
Differences in software can significantly skew results of medical scans commonly used in clinical care and research.
AMRI, PerkinElmer to Collaborate on Drug Discovery
Albany Molecular Research (AMRI) and PerkinElmer are collaborating to provide drug discovery infrastructure and services in AMRI's newly launched drug discovery center.
Exploring Living cells
JPK reports the exploration of living cells using nanoscale and single molecule techniques through the application of scanning probe microscopy.
Vulnerabilities in Genome's 'Dimmer Switches' Should Shed Light on Hundreds of Complex Diseases
Up to one-fifth of human DNA act as dimmer switches for nearby genes, but scientists have long been unable to identify precisely which mutations in these genetic control regions really matter in causing common diseases.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!