Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

Automating Deep Characterization of Biotherapeutic Proteins

Published: Thursday, June 19, 2014
Last Updated: Tuesday, July 01, 2014
Bookmark and Share
Thermo's new PepFinder software streamlines relative and absolute quantitation, and identification of proteins from biological samples using LC-MS.

As protein-based drugs become more important in treating major diseases, producers face the critical challenge of deeply characterizing these highly-complex molecules for efficacy and safety, as well as to meet quality requirements. Now there’s a new software tool designed to increase the speed, quality and confidence in comprehensive biotherapeutic protein characterization useful for drug development and production quality control.

Thermo Scientific PepFinder software is designed to streamline relative and absolute quantitation, and identification of proteins from biological samples using liquid chromatography-mass spectrometry.

Therapeutic proteins such as monoclonal antibodies, interferons, and insulin must be produced in biologically-active forms that  have proper folding and have specific post-translational modificiations (PTM). Traditional analysis of these complex biological molecules is highly complex and time-consuming. PepFinder software uses multidimensional dynamic search capabilities to automatically integrate complex data into concise reports.

“This is a new paradigm for peptide mapping data analysis,” said Ken Miller, vice president, marketing mass spectrometry for Thermo Fisher Scientific. “Customers can expect to reduce analysis time from weeks to hours in some cases, while generating high-confidence, comprehensive characterization reports, including known and unknown modifications.”

“Using PepFinder, a complex protein was analyzed with great details (including PTMs), and with great ease and speed!” said Shiaw-Lin (Billy) Wu, Head of Analytical Sciences at BioAnalytix and Research Associate Professor at Northeastern University.

PepFinder software, based on Mass Analyzer software licensed from Amgen, is compatible with Thermo Scientific LC-MS instruments, including the industry-leading high resolution, accurate-mass Orbitrap-based systems. This software is designed for fast, high-quality PTM profiles and peptide identifications based on a novel prediction algorithm. The software displays sequence coverage maps, automates relative changes in site specific PTMs for rapid monitoring of modifications in a single sample or across sample comparison. The software is written to provide an automated workflow for glycopeptides ID, disulfide bond mapping and other common PTM’s, including oxidation, deamidation and phosphorylation.  This can eliminate time consuming and error-prone manual PTM mapping.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thermo Fisher Scientific to Acquire Life Technologies
Transaction expected to create an unrivaled leader in serving research, specialty diagnostics and applied markets.
Wednesday, April 17, 2013
Hyperion Succesfully Completes Laboratory Infrastructure Project for Hellenic Petroleum
Hyperion and the Hellenic Petroleum Group have completed a major upgrade and extension project for the LIMS at three Greek petroleum refineries of the Group.
Wednesday, November 21, 2012
Thermo Fisher Scientific Reports Q3 Revenue and EPS Results
Revenue increased 5% to a record $3.09 billion for the third quarter with adjusted operating income rising 5% to $576 million.
Wednesday, October 24, 2012
Thermo Scientific Elects New Director to Board
Appointment of C. Martin Harris to Company’s board of directors.
Monday, March 26, 2012
Thermo Scientific Adds Pilgrim Software to Informatics Global Partner Alliance
Company provides enterprise compliance, risk and quality management software solutions for regulated industries.
Tuesday, April 05, 2011
Thermo Scientific to Launch Major New Innovative Informatics Offerings at PITTCON 2011
The company will showcase its new informatics offerings at PITTCON® 2011, being held from March 13-18 at the Georgia World Congress Center in Atlanta.
Tuesday, February 01, 2011
Thermo Fisher Scientific to Launch Major New Innovative Informatics Offerings at PITTCON 2011
Thermo Scientific showcases its new informatics offerings at PITTCON® 2011, being held from March 13-18 at the Georgia World Congress Center in Atlanta.
Sunday, January 30, 2011
Scientific News
Scientists Create World’s Largest Catalog of Human Genomic Variation
An international team of scientists from the 1000 Genomes Project Consortium has created the world’s largest catalog of genomic differences among humans, providing researchers with powerful clues to help them establish why some people are susceptible to various diseases.
New Autism Genes Are Revealed in Largest-Ever Study
Work draws more detailed picture of genetic risk, sheds light on sex differences in diagnosis.
Influenza A Viruses More Likely To Emerge In East Asia Than North America
Novel strains of influenza A are more likely to emerge in East Asia than in North America, according to a global analysis by the One Health Institute at the UC Davis School of Veterinary Medicine and EcoHealth Alliance.
Bone Risks Linked to Genetic Variants
A large-scale genomic study uncovered novel genetic variants and led researchers to an unexpected gene that affects bone density and fracture risk.
The Final Word on STAP
Researchers fail to replicate STAP study; computational analysis reveals genomic inconsistency.
Study Adds to Evidence That Viruses Are Alive
A new analysis supports the hypothesis that viruses are living entities that share a long evolutionary history with cells, researchers report.
CSI -- On The Metabolite's Trail
Bioinformaticians at the University of Jena make the most efficient search engine for molecular structures available online.
Potential Ovarian Cancer Biomarker Isolated
Researchers from North Carolina State University utilized a highly sensitive mass spectrometry analysis to identify and measure difficult-to-detect N-glycan biomarkers associated with ovarian cancers in stages I – IV.
Smartphone App to Monitor Serious Blood Disorder
A researcher from Florida Atlantic University has come up with a unique way to monitor sickle cell disease -- a serious blood disorder -- using a smart phone.
Network Control: Letting Noise Lead The Way
Research team leverage cells' noisy nature to keep them healthy.
Scroll Up
Scroll Down

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos